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protein coding gene - atg14 (SPAC25A8.02) - autophagy associated protein Atg14

Gene summary

Standard name
atg14
Systematic ID
SPAC25A8.02
Product
autophagy associated protein Atg14
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O42862
ORFeome ID
18/18E11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2102329..2104103 forward strand

Annotation

GO biological process

GO:0000045 - autophagosome assembly

References:

GO:0016240 - autophagosome membrane docking

References:

GO:0009267 - cellular response to starvation

References:

GO:0016236 - macroautophagy

References:

GO:0000423 - mitophagy

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GO cellular component

GO:0005776 - autophagosome

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GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0097629 - extrinsic component of omegasome membrane

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GO:0097632 - extrinsic component of phagophore assembly site membrane

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GO:0000329 - fungal-type vacuole membrane

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GO:0000407 - phagophore assembly site

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GO:0034271 - phosphatidylinositol 3-kinase complex, class III, type I

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GO molecular function

GO:0035014 - phosphatidylinositol 3-kinase regulator activity

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GO:0005515 - protein binding

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GO:0043495 - protein-membrane adaptor activity

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Multi-locus phenotype

FYPO:0002759 - abolished protein localization to pre-autophagosomal structure

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

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Genotypes:

FYPO:0001178 - loss of viability upon nitrogen starvation

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Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004159 - abnormal homologous chromosome segregation

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Genotypes:

FYPO:0002890 - abnormal horsetail nucleus morphology

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Genotypes:

FYPO:0004670 - abnormal macroautophagy during nitrogen starvation

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Genotypes:

FYPO:0002224 - abnormally arrested meiotic cell cycle

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Genotypes:

FYPO:0000380 - abolished macroautophagy

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Genotypes:

FYPO:0006295 - abolished macroautophagy during nitrogen starvation

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0002615 - decreased protein localization to pre-autophagosomal structure

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0008427 - increased protein level during cellular response to sulfur starvation

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Genotypes:

FYPO:0000238 - inviable cell upon G0 to G1 transition

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0006660 - loss of viability upon G0 to G1 transition

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Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

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Genotypes:

FYPO:0000478 - normal meiosis

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Genotypes:

FYPO:0006293 - normal protein localization via NVT pathway

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Genotypes:

FYPO:0006266 - normal vacuole size during vegetative growth

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0013765 - vps38 was previously annotated as atg14

Protein features

IDNameInterPro nameDB name
PF10186ATG14UV_resistance/autophagy_Atg14PFAM
PTHR13664BECLIN 1-ASSOCIATED AUTOPHAGY-RELATED KEY REGULATORPANTHER
CoilCoilCOILS

Orthologs

References / Literature

PMID:27737912 - Atg20- and Atg24-family proteins promote organelle autophagy in fission yeast.
Zhao D et al. J Cell Sci 2016 Nov 15;129(22):4289-4304
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:30116786 - Genetic regulation of mitotic competence in G 0 quiescent cells.
Sajiki K et al. Sci Adv 2018 Aug;4(8):eaat5685
PMID:31941401 - Atg38-Atg8 interaction in fission yeast establishes a positive feedback loop to promote autophagy.
Yu ZQ et al. Autophagy 2020 Nov;16(11):2036-2051
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.
Materne P et al. Elife 2015 Jun 22;4:e09008
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:32435206 - Posttranslational Arginylation Enzyme Arginyltransferase1 Shows Genetic Interactions With Specific Cellular Pathways in vivo .
Wiley DJ et al. Front Physiol 2020;11:427
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:26365378 - ESCRTs Cooperate with a Selective Autophagy Receptor to Mediate Vacuolar Targeting of Soluble Cargos.
Liu XM et al. Mol Cell 2015 Sep 17;59(6):1035-42
PMID:34499173 - Visual detection of binary, ternary and quaternary protein interactions in fission yeast using a Pil1 co-tethering assay.
Yu ZQ et al. J Cell Sci 2021 Oct 01;134(19)
PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.
Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69
PMID:26696398 - Autophagy is required for efficient meiosis progression and proper meiotic chromosome segregation in fission yeast.
Matsuhara H et al. Genes Cells 2016 Jan;21(1):65-87
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16169489 - Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.
Gregan J et al. Curr Biol 2005 Sep 20;15(18):1663-9
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:40395999 - Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast.
Ohtsuka H et al. Autophagy Rep 2022;1(1):51-64
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22146723 - Systematic localization study on novel proteins encoded by meiotically up-regulated ORFs in fission yeast.
Ikebe C et al. Biosci Biotechnol Biochem 2011;75(12):2364-70
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623