protein coding gene - rcf1 (SPAC25B8.07c) - cytochrome c oxidase assembly protein Rcf1

Gene summary

Standard name: rcf1
Systematic ID: SPAC25B8.07c
Product: cytochrome c oxidase assembly protein Rcf1
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: Q9UTB1
ORFeome ID: 02/02A10
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome I: 4166006..4167713 reverse strand

Annotation

GO biological process

GO:0097250 - mitochondrial respirasome assembly

References:

GO_REF:0000033

GO:0033617 - mitochondrial respiratory chain complex IV assembly

References:

GO_REF:0000024

GO cellular component

GO:0005743 - mitochondrial inner membrane

References:

GO_REF:0000024

GO:0005739 - mitochondrion

References:

PMID:16823372

Modification

MOD:01148 - ubiquitinylated lysine

References:

PMID:37970674

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

PMID:11152613

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

PMID:27984744

Quantitative gene expression

PBO:0006310 - protein level

References:

PMID:23101633

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0009031 - resistance to bleomycin

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0000764 - resistance to cycloheximide

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0009038 - resistance to egtazic acid

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0001453 - resistance to ethanol

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0001583 - resistance to lithium

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0000830 - resistance to vanadate

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0001501 - sensitive to brefeldin A

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0000087 - sensitive to hydrogen peroxide

References:

PMID:37787768

Genotypes:

rcf1delta

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

rcf1delta

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

PMID:23697806

Genotypes:

rcf1delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

ID	Name	InterPro name	DB name
PF04588	HIG_1_N	Hypoxia_induced_domain	Pfam
PS51503	HIG1	Hypoxia_induced_domain	PROSITE profiles
G3DSA:6.10.140.1320			CATH-Gene3D
PTHR12297		RCF1	PANTHER
Transmembrane alpha helix			DeepTMHMM
mobidb-lite-Disorder	disorder_prediction		MobiDB-Disorder

Orthologs

HIGD1B (HGNC:24318) Homo sapiens
HIGD2B (HGNC:26984) Homo sapiens
HIGD1C (HGNC:28044) Homo sapiens
HIGD2A (HGNC:28311) Homo sapiens
HIGD1A (HGNC:29527) Homo sapiens
rcf1 (SJAG_02975) Schizosaccharomyces japonicus
RCF1 (YML030W) Saccharomyces cerevisiae

References / Literature

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.: Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.: Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.: Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.: Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;