protein coding gene - SPAC25B8.11 - DNA-binding transcription factor

Gene summary

Systematic ID: SPAC25B8.11
Product: DNA-binding transcription factor
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: Q9UTA7
ORFeome ID: 27/27D11
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome I: 4173675..4176378 forward strand

Annotation

GO biological process

GO:0006357 - regulation of transcription by RNA polymerase II

References:

GO_REF:0000050

GO cellular component

GO:0005829 - cytosol

References:

PMID:16823372

GO:0005634 - nucleus

References:

PMID:16823372

GO molecular function

GO:0000981 - DNA-binding transcription factor activity, RNA polymerase II-specific

References:

GO_REF:0000002

GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding

References:

GO_REF:0000050

GO:0008270 - zinc ion binding

References:

GO_REF:0000002

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

PMID:25720772

MOD:01148 - ubiquitinylated lysine

References:

PMID:37970674

Protein features

PBO:0111744 - zf-fungal Zn(2)-Cys(6) binuclear cluster domain

PBO:0111743 - zinc finger protein

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

PMID:27984744

Quantitative gene expression

PBO:0006310 - protein level

References:

PMID:23101633

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000046 - decreased cell population growth

References:

PMID:23695302

Genotypes:

SPAC25B8.11+(wild type)[Overexpression]

FYPO:0006012 - elongated cell with abolished shmoo formation

References:

PMID:28410370

Genotypes:

SPAC25B8.11delta

FYPO:0006518 - loss of viability in G0

References:

PMID:33260998

Genotypes:

SPAC25B8.11delta

FYPO:0007553 - normal G1 to G0 transition

References:

PMID:33260998

Genotypes:

SPAC25B8.11delta

FYPO:0000764 - resistance to cycloheximide

References:

PMID:37787768

Genotypes:

SPAC25B8.11delta

FYPO:0002693 - resistance to diamide

References:

PMID:37787768

Genotypes:

SPAC25B8.11delta

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

PMID:37787768

Genotypes:

SPAC25B8.11delta

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

SPAC25B8.11delta

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

PMID:37787768

Genotypes:

SPAC25B8.11delta

FYPO:0001034 - resistance to tunicamycin

References:

PMID:28945192

Genotypes:

SPAC25B8.11delta

FYPO:0001234 - slow vegetative cell population growth

References:

PMID:25452419

Genotypes:

SPAC25B8.11delta

FYPO:0001492 - viable elongated vegetative cell

References:

PMID:23695302

Genotypes:

SPAC25B8.11+(wild type)[Overexpression]

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

SPAC25B8.11delta

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

PMID:23697806

Genotypes:

SPAC25B8.11delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

Protein features

ID	Name	InterPro name	DB name
PF00172	Zn_clus	Zn2Cys6_DnaBD	Pfam
PF04082	Fungal_trans	XnlR_reg_dom	Pfam
cd00067	GAL4	Zn2Cys6_DnaBD	CDD
cd12148	fungal_TF_MHR		CDD
PS00463	ZN2_CY6_FUNGAL_1	Zn2Cys6_DnaBD	PROSITE patterns
PS50048	ZN2_CY6_FUNGAL_2	Zn2Cys6_DnaBD	PROSITE profiles
SM00066	GAL4	Zn2Cys6_DnaBD	SMART
SM00906	Fungal_trans	XnlR_reg_dom	SMART
G3DSA:4.10.240.10		Zn2-C6_fun-type_DNA-bd_sf	CATH-Gene3D
SSF57701		Zn2-C6_fun-type_DNA-bd_sf	SUPERFAMILY
PTHR31668		Carb_Metab_Trans_Reg	PANTHER
mobidb-lite-Disorder	disorder_prediction		MobiDB-Disorder
mobidb-lite-Polar	disorder_prediction		MobiDB-Polar

Orthologs

DAL81 (YIR023W) Saccharomyces cerevisiae

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.: Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.: Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:22540037 - Predicting the fission yeast protein interaction network.: Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.: Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23695302 - Functional characterization of fission yeast transcription factors by overexpression analysis.: Vachon L et al. Genetics 2013 Aug;194(4):873-84
GO_REF:0000002 - Comments
PMID:41526646 - Development of a global screening system for detecting protein-protein interactions by luminescence complementation in fission yeast.: Azadeh F et al. Sci Rep 2026 Jan 12;
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.: Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.: Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.: Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.: Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .: Guydosh NR et al. Elife 2017 Sep 25;6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.: Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.: Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.: Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.: Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;