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protein coding gene - loz1 (SPAC25B8.19c) - DNA-binding transcription factor zf-C2H2 type, Loz1

Gene summary

Standard name
loz1
Systematic ID
SPAC25B8.19c
Product
DNA-binding transcription factor zf-C2H2 type, Loz1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC683.01c
UniProt ID
Q9UTA1
ORFeome ID
33/33B12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4192070..4195213 reverse strand

Annotation

GO biological process

GO:0006882 - intracellular zinc ion homeostasis

References:

GO:0000122 - negative regulation of transcription by RNA polymerase II

References:

GO cellular component

GO:0005634 - nucleus

References:

GO molecular function

GO:0001227 - DNA-binding transcription repressor activity, RNA polymerase II-specific

References:

GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding

References:

GO:0043565 - sequence-specific DNA binding

References:

GO:0008270 - zinc ion binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0006144 - decreased cellular gluconate level

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0006131 - increased cellular gluconate level

References:

Genotypes:

FYPO:0001535 - normal cellular zinc level

References:

Genotypes:

Protein features

PBO:0111745 - zf-C2H2 type

PBO:0111743 - zinc finger protein

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0002003 - abolished RNA polymerase II proximal promoter sequence-specific DNA binding

References:

Genotypes:

FYPO:0000046 - decreased cell population growth

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0009075 - decreased cell population growth on fructose carbon source

References:

Genotypes:

FYPO:0009100 - decreased cell population growth on glycerol and galactose carbon source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

References:

Genotypes:

FYPO:0000658 - decreased DNA binding

References:

Genotypes:

FYPO:0005018 - decreased double-stranded DNA binding

References:

Genotypes:

FYPO:0003008 - decreased repression of transcription during cellular response to zinc

References:

Genotypes:

FYPO:0007049 - decreased RNA level during stress response to zinc ion

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0006140 - increased cellular 1-eicosenoylglycerophosphocholine (20:1n9) level

References:

Genotypes:

FYPO:0006137 - increased cellular 1-stearoylglycerophosphocholine (18:0) level

References:

Genotypes:

FYPO:0006136 - increased cellular 2,3-butanediol level

References:

Genotypes:

FYPO:0006139 - increased cellular 2-myristoylglycerophosphocholine level

References:

Genotypes:

FYPO:0006134 - increased cellular 2-palmitoleoylglycerophosphoinositol level

References:

Genotypes:

FYPO:0006138 - increased cellular cis-aconitate level

References:

Genotypes:

FYPO:0006135 - increased cellular citrate level

References:

Genotypes:

FYPO:0006131 - increased cellular gluconate level

References:

Genotypes:

FYPO:0003516 - increased cellular L-ergothioneine level during vegetative growth

References:

Genotypes:

FYPO:0006132 - increased cellular mevalonate level

References:

Genotypes:

FYPO:0006133 - increased cellular phytosphingosine level

References:

Genotypes:

FYPO:0001552 - increased cellular zinc level

References:

Genotypes:

FYPO:0006828 - increased cytosolic zinc level

References:

Genotypes:

FYPO:0007092 - increased DNA binding during cellular response to zinc ion

References:

Genotypes:

FYPO:0007091 - increased negative regulation of transcription by zinc

References:

Genotypes:

FYPO:0007052 - increased protein level during stress response to zinc ion

References:

Genotypes:

FYPO:0005291 - increased RNA level during cellular response to zinc ion

References:

Genotypes:

FYPO:0001547 - increased RNA level during cellular response to zinc ion starvation

References:

Genotypes:

FYPO:0007050 - increased RNA level during stress response to zinc ion

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0002620 - normal growth on trichostatin A

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0006830 - normal RNA level during cellular response to replete zinc

References:

Genotypes:

FYPO:0006829 - normal RNA level during cellular response to zinc ion starvation

References:

Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

References:

Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0005968 - resistance to sodium chloride

References:

Genotypes:

FYPO:0005266 - resistance to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009040 - resistance to tea tree oil

References:

Genotypes:

FYPO:0002767 - resistance to terbinafine

References:

Genotypes:

FYPO:0000327 - resistance to trichostatin A

References:

Genotypes:

FYPO:0009067 - sensitive to amorolfine

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002617 - sensitive to sodium butyrate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0000648 - viable small vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0000110 - orthologs cannot be distinguished

Protein features

IDNameInterPro nameDB name
PF00096zf-C2H2Znf_C2H2_typePFAM
PS00028ZINC_FINGER_C2H2_1Znf_C2H2_typePROSITE_PATTERNS
PS50157ZINC_FINGER_C2H2_2Znf_C2H2_typePROSITE_PROFILES
SM00355c2h2final6Znf_C2H2_typeSMART
G3DSA:3.30.160.60:FF:000125FUNFAM
SSF57667beta-beta-alpha zinc fingersZnf_C2H2_sfSUPERFAMILY
G3DSA:3.30.160.60Classic Zinc FingerGENE3D
PTHR14003TRANSCRIPTIONAL REPRESSOR PROTEIN YYPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:28667014 - The gluconate shunt is an alternative route for directing glucose into the pentose phosphate pathway in fission yeast.
Corkins ME et al. J Biol Chem 2017 Aug 18;292(33):13823-13832
PMID:31515876 - The Loz1 transcription factor from Schizosaccharomyces pombe binds to Loz1 response elements and represses gene expression when zinc is in excess.
Wilson S et al. Mol Microbiol 2019 Dec;112(6):1701-1717
PMID:29529046 - Zinc transporters belonging to the Cation Diffusion Facilitator (CDF) family have complementary roles in transporting zinc out of the cytosol.
Choi S et al. PLoS Genet 2018 Mar;14(3):e1007262
PMID:23695302 - Functional characterization of fission yeast transcription factors by overexpression analysis.
Vachon L et al. Genetics 2013 Aug;194(4):873-84
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:26940262 - Zinc sensing and regulation in yeast model systems.
Wilson S et al. Arch Biochem Biophys 2016 Dec 01;611:30-36
PMID:23861937 - Genome-wide screening for genes associated with valproic acid sensitivity in fission yeast.
Zhang L et al. PLoS One 2013;8(7):e68738
PMID:24003116 - Zinc finger protein Loz1 is required for zinc-responsive regulation of gene expression in fission yeast.
Corkins ME et al. Proc Natl Acad Sci U S A 2013 Sep 17;110(38):15371-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:35924983 - Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.
Chatfield-Reed K et al. G3 (Bethesda) 2022 Aug 25;12(9)
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:36943461 - Zinc homeostasis in Schizosaccharomyces pombe.
Yao R et al. Arch Microbiol 2023 Mar 21;205(4):126
PMID:31239353 - Zinc-dependent activation of the Pho8 alkaline phosphatase in Schizosaccharomyces pombe .
Hu YM et al. J Biol Chem 2019 Aug 16;294(33):12392-12404
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39761853 - Regulation of sod1 mRNA and protein abundance by zinc in fission yeast is dependent on the CCR4-NOT complex.
Weeks AT et al. J Biol Chem 2025 Jan 04;:108156
PMID:39865413 - Conformational dynamics in specialized C 2 H 2 zinc finger domains enable zinc-responsive gene repression in S. pombe.
Wadhwa V et al. Protein Sci 2025 Feb;34(2):e70044
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24831008 - The double zinc finger domain and adjacent accessory domain from the transcription factor loss of zinc sensing 1 (loz1) are necessary for DNA binding and zinc sensing.
Ehrensberger KM et al. J Biol Chem 2014 Jun 27;289(26):18087-96
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;