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protein coding gene - cce1 (SPAC25G10.02) - cruciform-cutting endonuclease Cce1

Gene summary

Standard name
cce1
Systematic ID
SPAC25G10.02
Product
cruciform-cutting endonuclease Cce1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ydc2
UniProt ID
Q10423
ORFeome ID
09/09F08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4296936..4297905 reverse strand

Annotation

Complementation

PBO:0017769 - functionally complements E. coli rusA and ruvC

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GO biological process

GO:0032042 - mitochondrial DNA metabolic process

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005739 - mitochondrion

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GO molecular function

GO:0000402 - crossed form four-way junction DNA binding

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GO:0008821 - crossover junction DNA endonuclease activity

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GO:0070336 - flap-structured DNA binding

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GO:0000400 - four-way junction DNA binding

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GO:0005515 - protein binding

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GO:0000403 - Y-form DNA binding

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Protein features

PBO:0111804 - SAP domain

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000490 - abnormal mitochondrial genome maintenance

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Genotypes:

FYPO:0003667 - abolished crossover junction endodeoxyribonuclease activity

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Genotypes:

FYPO:0000659 - abolished DNA binding

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Genotypes:

FYPO:0003766 - aggregated mtDNA

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Genotypes:

FYPO:0003769 - decreased cellular mtDNA level

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Genotypes:

FYPO:0003767 - decreased crossover junction endodeoxyribonuclease activity

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Genotypes:

FYPO:0000658 - decreased DNA binding

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Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0000655 - normal DNA binding

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

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Genotypes:

FYPO:0000503 - normal mitotic recombination

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Genotypes:

FYPO:0003768 - normal protein localization to mitochondrion

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

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Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF09159Ydc2-catalytYdc2_catPfam
cd16963CCE1CDD
PS50800SAPSAP_domPROSITE profiles
SM00513SAPSAP_domSMART
G3DSA:3.30.420.10:FF:000160CATH-FunFam
G3DSA:3.30.420.10RNaseH_sfCATH-Gene3D
SSF53098RNaseH-like_sfSUPERFAMILY
SSF68906SAP_dom_sfSUPERFAMILY
PTHR28072Mrs1/Cce1PANTHER

Orthologs

References / Literature

PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.
Liu NN et al. Cell Res 2010 Aug;20(8):963-5
PMID:12823554 - Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance.
Sigala B et al. Eur J Biochem 2003 Jul;270(13):2837-47
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:9325108 - A new Holliday junction resolving enzyme from Schizosaccharomyces pombe that is homologous to CCE1 from Saccharomyces cerevisiae.
Whitby MC et al. J Mol Biol 1997 Oct 03;272(4):509-22
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:9837990 - Interaction of the resolving enzyme YDC2 with the four-way DNA junction.
White MF et al. Nucleic Acids Res 1998 Dec 15;26(24):5609-16
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:9421521 - Holliday junction resolvase in Schizosaccharomyces pombe has identical endonuclease activity to the CCE1 homologue YDC2.
Oram M et al. Nucleic Acids Res 1998 Jan 15;26(2):594-601
PMID:11726496 - Crystal structure of the fission yeast mitochondrial Holliday junction resolvase Ydc2.
Ceschini S et al. EMBO J 2001 Dec 03;20(23):6601-11
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:9450073 - A holliday junction endonuclease from fission yeast.
White MF et al. Biochem Soc Trans 1997 Nov;25(4):S645
PMID:9857040 - Substrate specificity of the SpCCE1 holliday junction resolvase of Schizosaccharomyces pombe.
Whitby MC et al. J Biol Chem 1998 Dec 25;273(52):35063-73
PMID:9343409 - Characterization of a Holliday junction-resolving enzyme from Schizosaccharomyces pombe.
White MF et al. Mol Cell Biol 1997 Nov;17(11):6465-71
PMID:10954073 - The Holliday junction resolvase SpCCE1 prevents mitochondrial DNA aggregation in Schizosaccharomyces pombe.
Doe CL et al. Mol Gen Genet 2000 Jul;263(6):889-97
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053