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protein coding gene - pcf3 (SPAC25H1.06) - histone H3-H4 chaperone, CAF assembly factor (CAF-1) complex WD repeat subunit C, Pcf3

Gene summary

Standard name
pcf3
Systematic ID
SPAC25H1.06
Product
histone H3-H4 chaperone, CAF assembly factor (CAF-1) complex WD repeat subunit C, Pcf3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9Y825
ORFeome ID
22/22B11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2531284..2532877 forward strand

Annotation

Comment

PBO:0001596 - destabilized in the absence of Pcf1

References:

Disease association

MONDO:0976123 - spermatogenic failure, X-linked, 9

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GO biological process

GO:0140861 - DNA repair-dependent chromatin remodeling

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GO:0006335 - DNA replication-dependent chromatin assembly

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GO:1990426 - mitotic recombination-dependent replication fork processing

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GO cellular component

GO:0033186 - CAF-1 complex

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GO:0000785 - chromatin

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GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0043596 - nuclear replication fork

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GO:0005634 - nucleus

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GO molecular function

GO:0000510 - H3-H4 histone complex chaperone activity

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GO:0042393 - histone binding

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Multi-locus phenotype

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

Protein features

PBO:0111751 - WD repeat protein

Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002346 - abnormal chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

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Genotypes:

FYPO:0006920 - decreased DNA recombination at mitotic DNA replication fork barriers

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Genotypes:

FYPO:0007005 - decreased DNA recombination downstream of mitotic DNA replication fork barriers

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0004026 - loss of template switch-mediated chromosomal rearrangement during replication fork processing

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0007479 - normal epigenetic heterochromatin inheritance

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007808 - resistance to valproic acid

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF12265CAF1C_H4-bdHistone-bd_RBBP4-like_NPFAM
PF00400WD40WD40_rptPFAM
PS50294WD_REPEATS_REGIONPROSITE_PROFILES
PS50082WD_REPEATS_2WD40_rptPROSITE_PROFILES
SM00320WD40_4WD40_rptSMART
SSF50978WD40 repeat-likeWD40_repeat_dom_sfSUPERFAMILY
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfGENE3D
PTHR22850WD40 REPEAT FAMILYWD_repeat_RBAP46/RBAP48/MSI1PANTHER

Orthologs

References / Literature

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18761674 - Fission yeast chromatin assembly factor 1 assists in the replication-coupled maintenance of heterochromatin.
Dohke K et al. Genes Cells 2008 Oct;13(10):1027-43
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:21211723 - Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci.
Yamane K et al. Mol Cell 2011 Jan 07;41(1):56-66
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:29899117 - Ccp1 modulates epigenetic stability at centromeres and affects heterochromatin distribution in Schizosaccharomyces pombe .
Lu M et al. J Biol Chem 2018 Aug 03;293(31):12068-12080
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:25313826 - The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1.
Pietrobon V et al. PLoS Biol 2014 Oct;12(10):e1001968
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:30667359 - Factors affecting template switch recombination associated with restarted DNA replication.
Jalan M et al. Elife 2019 Jan 22;8
PMID:38376141 - Disordered regions and folded modules in CAF-1 promote histone deposition in Schizosaccharomyces pombe .
Ouasti F et al. Elife 2024 Feb 20;12
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7