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protein coding gene - myh1 (SPAC26A3.02) - DNA adenine glycosylase Myh1

Gene summary

Standard name
myh1
Systematic ID
SPAC26A3.02
Product
DNA adenine glycosylase Myh1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
myh
UniProt ID
Q10159
ORFeome ID
24/24G10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3334882..3337714 forward strand

Annotation

Comment

PBO:0031881 - binding between Myh1 and Hus1 increases in response to oxidative stress

References:

PBO:0013448 - soluble Myh1 level decreases in presence of cisplatin or hydrogen peroxide

References:

Complementation

PBO:0091469 - functionally complements E. coli mutY

References:

PBO:0031882 - is not functionally complemented by human MUTYH

References:

Disease association

MONDO:0005575 - colorectal cancer

References:

MONDO:0012041 - familial adenomatous polyposis 2

References:

MONDO:0001056 - gastric cancer

References:

GO biological process

GO:0006284 - base-excision repair

References:

GO:0006285 - base-excision repair, AP site formation

References:

GO:0045007 - depurination

References:

GO:0006974 - DNA damage response

References:

GO:0006298 - mismatch repair

References:

GO cellular component

GO:0005634 - nucleus

References:

GO molecular function

GO:0051539 - 4 iron, 4 sulfur cluster binding

References:

GO:0034039 - 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity

References:

GO:0035485 - adenine/guanine mispair binding

References:

GO:0032357 - oxidized purine DNA binding

References:

GO:0005515 - protein binding

References:

GO:0000701 - purine-specific mismatch base pair DNA N-glycosylase activity

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Multi-locus phenotype

FYPO:0004254 - abnormal mitotic cell cycle regulation during cellular response to UV

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000102 - sensitive to cisplatin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0002345 - sensitive to oxaliplatin

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Genotypes:

FYPO:0002344 - sensitive to phleomycin

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

FYPO:0000648 - viable small vegetative cell

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Genotypes:

FYPO:0002377 - viable swollen vegetative cell

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004254 - abnormal mitotic cell cycle regulation during cellular response to UV

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0004677 - abolished purine-specific mismatch base pair DNA N-glycosylase activity

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0003240 - decreased phosphodiesterase I activity

References:

Genotypes:

FYPO:0002446 - decreased protein phosphorylation during cellular response to hydrogen peroxide

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0000256 - mutator

References:

Genotypes:

FYPO:0005297 - normal 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0005298 - normal oxidized DNA binding

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0007808 - resistance to valproic acid

References:

Genotypes:

FYPO:0007921 - sensitive to benzamidine

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00633HHHHhH_motifPfam
PF00730HhH-GPDHhH-GPD_domainPfam
PF10576EndIII_4Fe-2SEndonuclease3_FeS-loop_motifPfam
PF14815NUDIX_4MutY_CPfam
cd00056ENDO3cHhH-GPD_domainCDD
cd03431NUDIX_DNA_Glycosylase_C-MutYMutY_CCDD
PS00764ENDONUCLEASE_III_1Endouclease-III_FeS-bd_BSPROSITE patterns
PS01155ENDONUCLEASE_III_2Endonuclease-III-like_CS2PROSITE patterns
SM00478ENDO3cHhH-GPD_domainSMART
SM00525FESEndonuclease3_FeS-loop_motifSMART
G3DSA:1.10.1670.10:FF:000002CATH-FunFam
G3DSA:1.10.1670.10HhH_base_excis_CCATH-Gene3D
G3DSA:1.10.340.30CATH-Gene3D
G3DSA:3.90.79.10NUDIX_hydrolase-like_dom_sfCATH-Gene3D
SSF48150DNA_glycosylaseSUPERFAMILY
SSF55811NUDIX_hydrolase-like_dom_sfSUPERFAMILY
PTHR42944MIG/MutYPANTHER

Orthologs

References / Literature

PMID:18675827 - The tumor suppressor homolog in fission yeast, myh1(+), displays a strong interaction with the checkpoint gene rad1(+).
Jansson K et al. Mutat Res 2008 Sep 26;644(1-2):48-55
PMID:20816984 - A structural hinge in eukaryotic MutY homologues mediates catalytic activity and Rad9-Rad1-Hus1 checkpoint complex interactions.
Luncsford PJ et al. J Mol Biol 2010 Oct 29;403(3):351-70
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:11805113 - Functional interaction of MutY homolog with proliferating cell nuclear antigen in fission yeast, Schizosaccharomyces pombe.
Chang DY et al. J Biol Chem 2002 Apr 05;277(14):11853-8
PMID:23677513 - A role for Myh1 in DNA repair after treatment with strand-breaking and crosslinking chemotherapeutic agents.
Jansson K et al. Environ Mol Mutagen 2013 Jun;54(5):327-37
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24559510 - Interaction of apurinic/apyrimidinic endonuclease 2 (Apn2) with Myh1 DNA glycosylase in fission yeast.
Jin J et al. DNA Repair (Amst) 2014 Mar;15:1-10
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:21110984 - The role of MutY homolog (Myh1) in controlling the histone deacetylase Hst4 in the fission yeast Schizosaccharomyces pombe.
Chang DY et al. J Mol Biol 2011 Jan 21;405(3):653-65
PMID:36793083 - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes.
Mahrik L et al. Epigenetics Chromatin 2023 Feb 16;16(1):6
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PB_REF:0000003 - Disease Association Curation
GO_REF:0000002 - Comments
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:11683277 - Fission yeast (Schizosaccharomyces pombe) cells defective in the MutY-homologous glycosylase activity have a mutator phenotype and are sensitive to hydrogen peroxide.
Chang DY et al. Mol Genet Genomics 2001 Oct;266(2):336-42
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:9737967 - Characterization of the recombinant MutY homolog, an adenine DNA glycosylase, from yeast Schizosaccharomyces pombe.
Lu AL et al. J Biol Chem 1998 Sep 25;273(39):25098-105
PMID:15533944 - Interaction of checkpoint proteins Hus1/Rad1/Rad9 with DNA base excision repair enzyme MutY homolog in fission yeast, Schizosaccharomyces pombe.
Chang DY et al. J Biol Chem 2005 Jan 07;280(1):408-17
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:15988139 - The Shizosaccharomyces pombe homolog (SpMYH) of the Escherichia coli MutY is required for removal of guanine from 8-oxoguanine/guanine mispairs to prevent G:C to C:G transversions.
Doi T et al. J Radiat Res 2005 Jun;46(2):205-14