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protein coding gene - rga2 (SPAC26A3.09c) - RhoGAP Rga2

Gene summary

Standard name
rga2
Systematic ID
SPAC26A3.09c
Product
RhoGAP Rga2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC26A3.09
UniProt ID
Q10164
ORFeome ID
39/39C11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3348103..3352258 reverse strand

Annotation

Disease association

MONDO:0011444 - Duane retraction syndrome 2

References:

GO biological process

GO:0070610 - regulation of fungal-type cell wall (1->3)-alpha-glucan biosynthetic process

References:

GO:0007165 - signal transduction

References:

GO cellular component

GO:0032153 - cell division site

References:

GO:0051286 - cell tip

References:

GO:0005737 - cytoplasm

References:

GO:0035838 - growing cell tip

References:

GO:0110085 - mitotic actomyosin contractile ring

References:

GO:0120105 - mitotic actomyosin contractile ring, intermediate layer

References:

GO:0005886 - plasma membrane

References:

GO molecular function

GO:0005096 - GTPase activator activity

References:

GO:0008289 - lipid binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

Multi-locus phenotype

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0008309 - increased cell surface area at division

References:

Genotypes:

FYPO:0008308 - increased cell volume at division

References:

Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001884 - resistance to Calcofluor White

References:

Genotypes:

FYPO:0001882 - resistance to cell wall-degrading enzymes

References:

Genotypes:

FYPO:0001379 - resistance to tacrolimus

References:

Genotypes:

FYPO:0001473 - resistance to tacrolimus during salt stress

References:

Genotypes:

FYPO:0000079 - sensitive to caspofungin

References:

Genotypes:

FYPO:0003481 - viable elongated vegetative cell, elongated upon mitotic entry

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111759 - pleckstrin homology domain

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000190 - abnormal actin cortical patch localization during vegetative growth

References:

Genotypes:

FYPO:0000182 - abnormal cell wall organization during vegetative growth

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0001880 - abolished protein localization to cell division site

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Genotypes:

FYPO:0001585 - abolished protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0001081 - decreased cell wall alpha-glucan level

References:

Genotypes:

FYPO:0001082 - decreased cell wall beta-glucan level

References:

Genotypes:

FYPO:0001195 - decreased galactomannan level

References:

Genotypes:

FYPO:0001529 - decreased GTP binding

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0001885 - decreased protein phosphorylation during salt stress

References:

Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000587 - delayed onset of sporulation

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Genotypes:

FYPO:0001881 - elongated actin filaments

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0001084 - increased cell wall alpha-glucan level

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Genotypes:

FYPO:0001977 - increased cell wall galactomannan level

References:

Genotypes:

FYPO:0001035 - increased cell wall thickness during vegetative growth

References:

Genotypes:

FYPO:0001724 - increased GTP binding

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Genotypes:

FYPO:0001571 - increased protein-protein interaction

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Genotypes:

FYPO:0001406 - increased septum thickness

References:

Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0002482 - inviable spheroid vegetative cell

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0001294 - normal actin cortical patch localization

References:

Genotypes:

FYPO:0001883 - normal growth on caspofungin

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Genotypes:

FYPO:0001470 - normal growth on tacrolimus

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Genotypes:

FYPO:0001527 - normal GTP binding

References:

Genotypes:

FYPO:0001587 - normal protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0001266 - normal protein phosphorylation during cellular response to salt stress

References:

Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0001882 - resistance to cell wall-degrading enzymes

References:

Genotypes:

FYPO:0001450 - resistance to cold

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0005193 - resistance to torin1

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0000271 - sensitive to salt stress

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000086 - sensitive to tacrolimus

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0000022 - shmoo with long tip

References:

Genotypes:

FYPO:0000024 - stubby vegetative cell

References:

Genotypes:

FYPO:0001879 - thin cell

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0003481 - viable elongated vegetative cell, elongated upon mitotic entry

References:

Genotypes:

FYPO:0001877 - viable thin vegetative cell

References:

Genotypes:

FYPO:0001878 - viable thin, elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002197 - viable vegetative cell with abnormal cell shape

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF00169PHPH_domainPfam
PF00620RhoGAPRhoGAP_domPfam
cd04400RhoGAP_fBEM3CDD
PS50003PH_DOMAINPH_domainPROSITE profiles
PS50238RHOGAPRhoGAP_domPROSITE profiles
SM00233PHPH_domainSMART
SM00324RhoGAPRhoGAP_domSMART
G3DSA:1.10.555.10Rho_GTPase_activation_protCATH-Gene3D
G3DSA:2.30.29.30PH-like_dom_sfCATH-Gene3D
SSF48350Rho_GTPase_activation_protSUPERFAMILY
SSF50729SUPERFAMILY
PTHR23176Rho-GAPPANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMobiDB-Negative-Polyelectrolyte
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:30639107 - Reprogramming Cdr2-Dependent Geometry-Based Cell Size Control in Fission Yeast.
Facchetti G et al. Curr Biol 2019 Jan 21;29(2):350-358.e4
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28914606 - Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
McDonald NA et al. Elife 2017 Sep 15;6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:18793338 - Rga2 is a Rho2 GAP that regulates morphogenesis and cell integrity in S. pombe.
Villar-Tajadura MA et al. Mol Microbiol 2008 Nov;70(4):867-81
PMID:20164182 - Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein.
Soto T et al. J Biol Chem 2010 Apr 09;285(15):11516-25
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
GO_REF:0000002 - Comments
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:11737264 - Characterization of GTPase-activating proteins for the function of the Rho-family small GTPases in the fission yeast Schizosaccharomyces pombe.
Nakano K et al. Genes Cells 2001 Dec;6(12):1031-42