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protein coding gene - mga2 (SPAC26H5.05) - membrane sensing transcription coactivator, ankyrin repeat containing Mga2

Gene summary

Standard name
mga2
Systematic ID
SPAC26H5.05
Product
membrane sensing transcription coactivator, ankyrin repeat containing Mga2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13987
ORFeome ID
48/48D01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4125083..4128805 forward strand

Annotation

Disease association

MONDO:0013886 - cerebellar dysfunction with variable cognitive and behavioral abnormalities

References:

GO biological process

GO:0045834 - positive regulation of lipid metabolic process

References:

GO:0045944 - positive regulation of transcription by RNA polymerase II

References:

GO cellular component

GO:0098554 - cytoplasmic side of endoplasmic reticulum membrane

References:

GO:0000324 - fungal-type vacuole

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0003690 - double-stranded DNA binding

References:

GO:0003713 - transcription coactivator activity

References:

Miscellaneous functional group

PBO:0002382 - membrane-tethered transcription factor

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0000164 - abnormal cell separation after cytokinesis

References:

Genotypes:

FYPO:0000229 - cut

References:

Genotypes:

FYPO:0004880 - decreased level of lipid metabolism gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0005187 - decreased level of transport gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0001274 - decreased protein level during cellular response to hypoxia

References:

Genotypes:

FYPO:0001422 - decreased protein processing during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002989 - increased level of transmembrane transport gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0005995 - increased lncRNA level

References:

Genotypes:

FYPO:0002552 - lipid droplets present in decreased numbers

References:

Genotypes:

FYPO:0006791 - lipid droplets present in normal numbers

References:

Genotypes:

FYPO:0003751 - normal nuclear envelope morphology

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0001245 - sensitive to cobalt

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0007474 - variable cell size at division

References:

Genotypes:

FYPO:0008362 - variable cellular lipid droplet content

References:

Genotypes:

Protein features

PBO:0111781 - ankyrin repeat protein

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0002398 - abnormal actin cytoskeleton during vegetative growth

References:

Genotypes:

FYPO:0000164 - abnormal cell separation after cytokinesis

References:

Genotypes:

FYPO:0002399 - abnormal microtubule cytoskeleton

References:

Genotypes:

FYPO:0000443 - abnormal protein localization during vegetative growth

References:

Genotypes:

FYPO:0000229 - cut

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0005584 - decreased cellular diglyceride level

References:

Genotypes:

FYPO:0002324 - decreased cellular lanosterol level

References:

Genotypes:

FYPO:0005591 - decreased cellular lysophosphatidylcholine level

References:

Genotypes:

FYPO:0005592 - decreased cellular lysophosphatidylethanolamine level

References:

Genotypes:

FYPO:0005588 - decreased cellular phosphoinositide level

References:

Genotypes:

FYPO:0005585 - decreased cellular triglyceride level during vegetative growth

References:

Genotypes:

FYPO:0005580 - decreased level of lipid metabolism gene mRNA during cellular response to hypoxia

References:

Genotypes:

FYPO:0004880 - decreased level of lipid metabolism gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0005187 - decreased level of transport gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0001911 - decreased protein glycosylation during vegetative growth

References:

Genotypes:

FYPO:0008249 - decreased protein localization to chromatin at protein coding gene

References:

Genotypes:

FYPO:0003935 - decreased protein localization to Golgi apparatus, with protein mislocalized to endoplasmic reticulum

References:

Genotypes:

FYPO:0001422 - decreased protein processing during vegetative growth

References:

Genotypes:

FYPO:0001259 - decreased RNA level during cellular response to hypoxia

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000133 - elongated multinucleate vegetative cell

References:

Genotypes:

FYPO:0009095 - increased cell population growth on fructose carbon source

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0002318 - increased cellular ergosterol level

References:

Genotypes:

FYPO:0005593 - increased cellular inositol phosphorylceramide level

References:

Genotypes:

FYPO:0005587 - increased cellular phosphatidic acid level

References:

Genotypes:

FYPO:0005586 - increased cellular phosphatidylethanolamine level

References:

Genotypes:

FYPO:0005583 - increased fatty acid saturation

References:

Genotypes:

FYPO:0002989 - increased level of transmembrane transport gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0005995 - increased lncRNA level

References:

Genotypes:

FYPO:0000847 - increased protein degradation during vegetative growth

References:

Genotypes:

FYPO:0006068 - increased protein localization to endoplasmic reticulum

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0000650 - increased septation index

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

References:

Genotypes:

FYPO:0002552 - lipid droplets present in decreased numbers

References:

Genotypes:

FYPO:0006791 - lipid droplets present in normal numbers

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0005590 - normal cellular cardiolipin level

References:

Genotypes:

FYPO:0005595 - normal cellular ceramide level

References:

Genotypes:

FYPO:0001506 - normal cellular phosphatidylcholine level

References:

Genotypes:

FYPO:0005589 - normal cellular phosphatidylserine level

References:

Genotypes:

FYPO:0002448 - normal Dsc complex assembly

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0005582 - normal monoacylglycerol phosphate chain length

References:

Genotypes:

FYPO:0001215 - normal protein complex assembly during vegetative growth

References:

Genotypes:

FYPO:0002800 - normal protein degradation during vegetative growth

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0004422 - normal protein phosphorylation

References:

Genotypes:

FYPO:0001668 - normal protein processing during vegetative growth

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009039 - resistance to potassium chloride

References:

Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0005968 - resistance to sodium chloride

References:

Genotypes:

FYPO:0009089 - resistance to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0005266 - resistance to sodium dodecyl sulfate

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0001245 - sensitive to cobalt

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0007358 - sensitive to heavy water

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000086 - sensitive to tacrolimus

References:

Genotypes:

FYPO:0007938 - sensitive to tea tree oil

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0002546 - sensitive to trichostatin A

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0005581 - short glycerophospholipids

References:

Genotypes:

FYPO:0002400 - single microtubule bundle during mitotic interphase

References:

Genotypes:

FYPO:0007474 - variable cell size at division

References:

Genotypes:

FYPO:0007660 - variable cell size at division with subsequent division at abnormal size

References:

Genotypes:

FYPO:0008362 - variable cellular lipid droplet content

References:

Genotypes:

FYPO:0002106 - viable stubby vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002197 - viable vegetative cell with abnormal cell shape

References:

Genotypes:

FYPO:0001510 - viable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF01833TIGIPT_domPfam
PF12796Ank_2Ankyrin_rptPfam
PF25603SPT23_MGA2_DBDSPT23_MGA2_DBDPfam
cd00102IPTCDD
PS50088ANK_REPEATAnkyrin_rptPROSITE profiles
PS50297ANK_REP_REGIONPROSITE profiles
SM00248ANKAnkyrin_rptSMART
SM00429IPTIPT_domSMART
G3DSA:1.25.40.20Ankyrin_rpt-contain_sfCATH-Gene3D
G3DSA:2.60.40.10Ig-like_foldCATH-Gene3D
SSF48403Ankyrin_rpt-contain_sfSUPERFAMILY
SSF81296Ig_E-setSUPERFAMILY
PTHR23335PANTHER
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:12615979 - Polo boxes form a single functional domain that mediates interactions with multiple proteins in fission yeast polo kinase.
Reynolds N et al. J Cell Sci 2003 Apr 01;116(Pt 7):1377-87
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:22806344 - Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast.
Ryuko S et al. Mol Genet Genomics 2012 Aug;287(8):651-62
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
GO_REF:0000036 - Manual annotations that require more than one source of functional data to support the assignment of the associated GO term
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:27053105 - Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.
Burr R et al. J Biol Chem 2016 Jun 03;291(23):12171-83
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:28202541 - Coordinate Regulation of Yeast Sterol Regulatory Element-binding Protein (SREBP) and Mga2 Transcription Factors.
Burr R et al. J Biol Chem 2017 Mar 31;292(13):5311-5324
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:38780300 - Nitrogen availability is important for preventing catastrophic mitosis in fission yeast.
Zemlianski V et al. J Cell Sci 2024 May 23;
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:21850271 - Genome-wide screening for genes associated with FK506 sensitivity in fission yeast.
Ma Y et al. PLoS One 2011;6(8):e23422
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:32142608 - Mutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water.
Kampmeyer C et al. ACS Synth Biol 2020 Apr 17;9(4):733-748
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:39652606 - Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast.
Marešová A et al. PLoS Genet 2024 Dec 09;20(12):e1011509
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:33419777 - Identification of mutants with increased variation in cell size at onset of mitosis in fission yeast.
Scotchman E et al. J Cell Sci 2021 Feb 11;134(3)
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39