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protein coding gene - pot1 (SPAC26H5.06) - shelterin complex ssDNA binding subunit Pot1

Gene summary

Standard name
pot1
Systematic ID
SPAC26H5.06
Product
shelterin complex ssDNA binding subunit Pot1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13988
ORFeome ID
45/45H02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4129224..4132350 forward strand

Annotation

Disease association

MONDO:0957264 - cerebroretinal microangiopathy with calcifications and cysts 3

References:

MONDO:0957263 - pulmonary fibrosis and/or bone marrow failure syndrome, telomere-related, 8

References:

MONDO:0014368 - tumor predisposition syndrome 3

References:

GO biological process

GO:0032210 - regulation of telomere maintenance via telomerase

References:

GO:0016233 - telomere capping

References:

GO:0000723 - telomere maintenance

References:

GO cellular component

GO:0140445 - chromosome, telomeric repeat region

References:

GO:0005634 - nucleus

References:

GO:0070187 - shelterin complex

References:

GO:0000782 - telomere cap complex

References:

GO molecular function

GO:0098505 - G-rich strand telomeric DNA binding

References:

GO:0005515 - protein binding

References:

GO:0043047 - single-stranded telomeric DNA binding

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GO:0010521 - telomerase inhibitor activity

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Modification

MOD:01149 - sumoylated lysine

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Multi-locus phenotype

FYPO:0000481 - abnormal mitotic recombination

References:

Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0002702 - circularized chromosome

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

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Genotypes:

FYPO:0004985 - entangled telomeres during vegetative growth

References:

Genotypes:

FYPO:0002573 - increased number of Ssb1 foci

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0003106 - stable shortened telomeres during vegetative growth

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Genotypes:

Protein features

PBO:0111871 - OB fold

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0002702 - circularized chromosome

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0000658 - decreased DNA binding

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

Genotypes:

FYPO:0002262 - inviable after spore germination, multiple cell divisions, elongated cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002887 - normal protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0005553 - normal single-stranded telomeric DNA binding

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF02765POT1Telomer_end-bd_POT1/Cdc13Pfam
PF16686POT1PCPOT1PCPfam
cd04497hPOT1_OB1_likeCDD
SM00976Telo_bindTelomer_end-bd_POT1/Cdc13SMART
G3DSA:2.40.50.140:FF:000303CATH-FunFam
G3DSA:2.40.50.140:FF:000426CATH-FunFam
G3DSA:2.40.50.140NA-bd_OB-foldCATH-Gene3D
SSF50249NA-bd_OB-foldSUPERFAMILY
PTHR14513POT1PANTHER

Orthologs

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:21098121 - Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation.
Takahashi K et al. Mol Cell Biol 2011 Feb;31(3):495-506
PMID:14614509 - DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA.
Lei M et al. Nature 2003 Nov 13;426(6963):198-203
PMID:25398909 - Fission yeast Cactin restricts telomere transcription and elongation by controlling Rap1 levels.
Lorenzi LE et al. EMBO J 2015 Jan 02;34(1):115-29
PMID:26063574 - Minishelterins separate telomere length regulation and end protection in fission yeast.
Pan L et al. Genes Dev 2015 Jun 01;29(11):1164-74
PMID:29298360 - Chromosome passenger complex is required for the survival of cells with ring chromosomes in fission yeast.
Habib AGK et al. PLoS One 2018;13(1):e0190523
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:27245259 - Telomerase RNA is more than a DNA template.
Webb CJ et al. RNA Biol 2016 Aug 02;13(8):683-9
PMID:34255844 - The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.
Liu J et al. Nucleic Acids Res 2021 Aug 20;49(14):8110-8119
PMID:24925530 - Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association.
Garg M et al. EMBO Rep 2014 Aug;15(8):871-7
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:22313747 - A double mutant between fission yeast telomerase and RecQ helicase is sensitive to thiabendazole, an anti-microtubule drug.
Ukimori S et al. Biosci Biotechnol Biochem 2012;76(2):264-9
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22978652 - A small molecule inhibitor of Pot1 binding to telomeric DNA.
Altschuler SE et al. Biochemistry 2012 Oct 09;51(40):7833-45
PMID:17429064 - Fission yeast Taz1 and RPA are synergistically required to prevent rapid telomere loss.
Kibe T et al. Mol Biol Cell 2007 Jun;18(6):2378-87
PMID:23297345 - Fission yeast RecQ helicase Rqh1 is required for the maintenance of circular chromosomes.
Nanbu T et al. Mol Cell Biol 2013 Mar;33(6):1175-87
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:19714219 - Fission yeast Tel1(ATM) and Rad3(ATR) promote telomere protection and telomerase recruitment.
Moser BA et al. PLoS Genet 2009 Aug;5(8):e1000622
PMID:29596531 - Insights into DNA substrate selection by APOBEC3G from structural, biochemical, and functional studies.
Ziegler SJ et al. PLoS One 2018;13(3):e0195048
PMID:24240238 - Elimination of shelterin components bypasses RNAi for pericentric heterochromatin assembly.
Tadeo X et al. Genes Dev 2013 Nov 15;27(22):2489-99
PMID:24469396 - Rad51-dependent aberrant chromosome structures at telomeres and ribosomal DNA activate the spindle assembly checkpoint.
Nakano A et al. Mol Cell Biol 2014 Apr;34(8):1389-97
PMID:24013504 - Tpz1 controls a telomerase-nonextendible telomeric state and coordinates switching to an extendible state via Ccq1.
Jun HI et al. Genes Dev 2013 Sep 01;27(17):1917-31
PMID:29681468 - Discrimination against RNA Backbones by a ssDNA Binding Protein.
Lloyd NR et al. Structure 2018 May 01;26(5):722-733.e2
PMID:17209013 - Role of SUMO in the dynamics of telomere maintenance in fission yeast.
Xhemalce B et al. Proc Natl Acad Sci U S A 2007 Jan 16;104(3):893-8
PMID:12463756 - Cooperative binding of single-stranded telomeric DNA by the Pot1 protein of Schizosaccharomyces pombe.
Lei M et al. Biochemistry 2002 Dec 10;41(49):14560-8
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:17715303 - Protection of telomeres by a conserved Stn1-Ten1 complex.
Martín V et al. Proc Natl Acad Sci U S A 2007 Aug 28;104(35):14038-43
PMID:18535244 - Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length.
Miyoshi T et al. Science 2008 Jun 06;320(5881):1341-4
PMID:23028377 - Factors that promote H3 chromatin integrity during transcription prevent promiscuous deposition of CENP-A(Cnp1) in fission yeast.
Choi ES et al. PLoS Genet 2012 Sep;8(9):e1002985
PMID:21911358 - DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe.
Nandakumar J et al. Nucleic Acids Res 2012 Jan;40(1):235-44
PMID:26365187 - Dissecting Fission Yeast Shelterin Interactions via MICro-MS Links Disruption of Shelterin Bridge to Tumorigenesis.
Liu J et al. Cell Rep 2015 Sep 29;12(12):2169-80
PMID:26041456 - RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends.
Audry J et al. EMBO J 2015 Jul 14;34(14):1942-58
PMID:19214192 - Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.
Moser BA et al. EMBO J 2009 Apr 08;28(7):810-20
PMID:24711392 - SUMOylation regulates telomere length by targeting the shelterin subunit Tpz1(Tpp1) to modulate shelterin-Stn1 interaction in fission yeast.
Miyagawa K et al. Proc Natl Acad Sci U S A 2014 Apr 22;111(16):5950-5
PMID:26465752 - Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres.
Nanbu T et al. PLoS One 2015;10(10):e0140456
PMID:26305931 - Telomerase RNA stem terminus element affects template boundary element function, telomere sequence, and shelterin binding.
Webb CJ et al. Proc Natl Acad Sci U S A 2015 Sep 08;112(36):11312-7
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:26988418 - The proper connection between shelterin components is required for telomeric heterochromatin assembly.
Wang J et al. Genes Dev 2016 Apr 01;30(7):827-39
PMID:25330395 - Tpz1-Ccq1 and Tpz1-Poz1 interactions within fission yeast shelterin modulate Ccq1 Thr93 phosphorylation and telomerase recruitment.
Harland JL et al. PLoS Genet 2014 Oct;10(10):e1004708
PMID:29121084 - Fission yeast strains with circular chromosomes require the 9-1-1 checkpoint complex for the viability in response to the anti-cancer drug 5-fluorodeoxyuridine.
Shamim HM et al. PLoS One 2017;12(11):e0187775
PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.
Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146
PMID:25074378 - The telomeric protein Pot1 from Schizosaccharomyces pombe binds ssDNA in two modes with differing 3' end availability.
Dickey TH et al. Nucleic Acids Res 2014 Sep;42(15):9656-65
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:18722173 - Chromosome fusions following telomere loss are mediated by single-strand annealing.
Wang X et al. Mol Cell 2008 Aug 22;31(4):463-473
PMID:23201273 - Nonspecific recognition is achieved in Pot1pC through the use of multiple binding modes.
Dickey TH et al. Structure 2013 Jan 08;21(1):121-132
PMID:26093291 - Long G2 accumulates recombination intermediates and disturbs chromosome segregation at dysfunction telomere in Schizosaccharomyces pombe.
Habib AG et al. Biochem Biophys Res Commun 2015 Aug 14;464(1):140-6
PMID:35849625 - Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex.
Sun H et al. PLoS Genet 2022 Jul;18(7):e1010308
PMID:41201383 - Combinatorial Analyses of Pre-mRNA Splicing-Defective Mutants Reveal Differential Quantitative Requirements for Shelterin in Distinct Telomere Functions.
Takeuchi M et al. Genes Cells 2025 Nov;30(6):e70064
PMID:15637058 - Extended DNA binding site in Pot1 broadens sequence specificity to allow recognition of heterogeneous fission yeast telomeres.
Trujillo KM et al. J Biol Chem 2005 Mar 11;280(10):9119-28
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:25131669 - Telomerase activation after recruitment in fission yeast.
Armstrong CA et al. Curr Biol 2014 Sep 08;24(17):2006-11
PMID:29410177 - Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1.
Sugihara A et al. Biochem Biophys Res Commun 2018 Feb 19;496(4):1284-1290
PMID:31131414 - Casein kinase 2 regulates telomere protein complex formation through Rap1 phosphorylation.
Inoue H et al. Nucleic Acids Res 2019 Jul 26;47(13):6871-6884
PMID:11349150 - Pot1, the putative telomere end-binding protein in fission yeast and humans.
Baumann P et al. Science 2001 May 11;292(5519):1171-5