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protein coding gene - ssm4 (SPAC27D7.13c) - dynactin microtubule-binding subunit, p150-Glued Ssm4

Gene summary

Standard name
ssm4
Systematic ID
SPAC27D7.13c
Product
dynactin microtubule-binding subunit, p150-Glued Ssm4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC637.01c
UniProt ID
O42667
ORFeome ID
27/27E02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4534674..4536736 reverse strand

Annotation

Disease association

MONDO:0019502 - autosomal recessive non-syndromic intellectual disability

References:

MONDO:0008678 - Williams syndrome

References:

GO biological process

GO:0030989 - dynein-driven meiotic oscillatory nuclear movement

References:

GO:0007127 - meiosis I

References:

GO:0000743 - nuclear migration involved in conjugation with cellular fusion

References:

GO cellular component

GO:0051285 - cell cortex of cell tip

References:

GO:1903754 - cortical microtubule plus-end

References:

GO:0005881 - cytoplasmic microtubule

References:

GO:0005869 - dynactin complex

References:

GO:0035974 - meiotic spindle pole body

References:

GO:0015630 - microtubule cytoskeleton

References:

GO:0072686 - mitotic spindle

References:

GO:0110092 - nucleus leading edge

References:

GO molecular function

GO:0005515 - protein binding

References:

Modification

MOD:00595 - mannosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0003066 - abnormal sporulation resulting in formation of ascus with fewer than four spores

References:

Genotypes:

FYPO:0006130 - abolished protein localization to microtubule during meiosis

References:

Genotypes:

FYPO:0007147 - normal Mei2 nuclear dot formation

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Genotypes:

Qualitative gene expression

PomGeneEx:0000015 - RNA absent

References:

PomGeneEx:0000011 - RNA level increased

References:

PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003066 - abnormal sporulation resulting in formation of ascus with fewer than four spores

References:

Genotypes:

FYPO:0001894 - abnormal sporulation resulting in formation of ascus with more or fewer than four spores

References:

Genotypes:

FYPO:0000681 - abnormal sporulation resulting in formation of two-spore ascus

References:

Genotypes:

FYPO:0007530 - abolished histone H3-K9 dimethylation at heterochromatin island at meiotic gene during vegetative growth

References:

Genotypes:

FYPO:0000927 - abolished horsetail movement

References:

Genotypes:

FYPO:0005988 - abolished microtubule anchoring at cell cortex of cell tip during meiotic cell cycle

References:

Genotypes:

FYPO:0003269 - abolished protein localization to microtubule during vegetative growth

References:

Genotypes:

FYPO:0005814 - abolished protein localization to microtubule plus-end

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0007531 - decreased histone H3-K9 dimethylation at heterochromatin island at meiotic gene during vegetative growth

References:

Genotypes:

FYPO:0003025 - decreased homologous chromosome pairing

References:

Genotypes:

FYPO:0003179 - decreased intragenic meiotic recombination

References:

Genotypes:

FYPO:0000929 - decreased protein localization to cell cortex during vegetative growth

References:

Genotypes:

FYPO:0000937 - decreased protein localization to cortical microtubule cytoskeleton during vegetative growth

References:

Genotypes:

FYPO:0000933 - decreased protein localization to microtubule cytoskeleton during vegetative growth

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0000636 - increased cell population growth rate

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0005383 - normal duration of meiosis I

References:

Genotypes:

FYPO:0006128 - normal duration of meiosis II

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0000964 - normal growth on thiabendazole

References:

Genotypes:

FYPO:0000760 - normal mating

References:

Genotypes:

FYPO:0004093 - normal meiotic telomere clustering

References:

Genotypes:

FYPO:0005991 - normal protein localization to cell cortex of cell tip during karyogamy

References:

Genotypes:

FYPO:0003541 - normal protein localization to meiotic spindle pole body

References:

Genotypes:

FYPO:0004764 - normal protein localization to meiotic spindle pole body during meiosis I

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009035 - resistance to formamide

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000022 - shmoo with long tip

References:

Genotypes:

FYPO:0000678 - unequal homologous chromosome segregation

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF01302CAP_GLYCAP-Gly_domainPFAM
PS00845CAP_GLY_1CAP-Gly_domainPROSITE_PATTERNS
PS50245CAP_GLY_2CAP-Gly_domainPROSITE_PROFILES
SM01052CAP_GLY_2CAP-Gly_domainSMART
G3DSA:2.30.30.190:FF:000014FUNFAM
SSF74924Cap-Gly domainCAP-Gly_dom_sfSUPERFAMILY
G3DSA:2.30.30.190CAP-Gly_dom_sfGENE3D
PTHR18916DYNACTIN 1-RELATED MICROTUBULE-BINDINGPANTHER
CoilCoilCOILS

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:33574613 - TOR targets an RNA processing network to regulate facultative heterochromatin, developmental gene expression and cell proliferation.
Wei Y et al. Nat Cell Biol 2021 Mar;23(3):243-256
PMID:31974447 - Formation of S. pombe Erh1 homodimer mediates gametogenic gene silencing and meiosis progression.
Hazra D et al. Sci Rep 2020 Jan 23;10(1):1034
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20298435 - Contribution of dynein light intermediate and intermediate chains to subcellular localization of the dynein-dynactin motor complex in Schizosaccharomyces pombe.
Fujita I et al. Genes Cells 2010 Apr 01;15(4):359-72
PMID:23755176 - Proper microtubule structure is vital for timely progression through meiosis in fission yeast.
Yamashita A et al. PLoS One 2013;8(6):e65082
PMID:40185772 - A bifunctional snoRNA with separable activities in guiding rRNA 2'-O-methylation and scaffolding gametogenesis effectors.
Leroy E et al. Nat Commun 2025 Apr 05;16(1):3250
PMID:16762840 - Swi6/HP1 recruits a JmjC domain protein to facilitate transcription of heterochromatic repeats.
Zofall M et al. Mol Cell 2006 Jun 09;22(5):681-92
PB_REF:0000003 - Disease Association Curation
PMID:21317872 - Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast.
Sugiyama T et al. EMBO J 2011 Mar 16;30(6):1027-39
PMID:25869666 - Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways.
Scheffler K et al. J Cell Biol 2015 Apr 13;209(1):47-58
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23658229 - Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast.
Sugiyama T et al. Nucleic Acids Res 2013 Jul;41(13):6674-86
PMID:16624923 - Fission yeast Num1p is a cortical factor anchoring dynein and is essential for the horse-tail nuclear movement during meiotic prophase.
Yamashita A et al. Genetics 2006 Jul;173(3):1187-96
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:26518661 - The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast.
Sadeghi L et al. EMBO Rep 2015 Dec;16(12):1673-87
PMID:28765164 - Selective termination of lncRNA transcription promotes heterochromatin silencing and cell differentiation.
Touat-Todeschini L et al. EMBO J 2017 Sep 01;36(17):2626-2641
PMID:29424342 - YTH-RNA-binding protein prevents deleterious expression of meiotic proteins by tethering their mRNAs to nuclear foci.
Shichino Y et al. Elife 2018 Feb 09;7
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:24210919 - Mtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance.
Lee NN et al. Cell 2013 Nov 21;155(5):1061-74
PMID:22912768 - Rhn1, a nuclear protein, is required for suppression of meiotic mRNAs in mitotically dividing fission yeast.
Sugiyama T et al. PLoS One 2012;7(8):e42962
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:24920274 - Meiotic long non-coding meiRNA accumulates as a dot at its genetic locus facilitated by Mmi1 and plays as a decoy to lure Mmi1.
Shichino Y et al. Open Biol 2014 Jun;4(6):140022
PMID:28841135 - Ubiquitination-dependent control of sexual differentiation in fission yeast.
Simonetti F et al. Elife 2017 Aug 25;6
PMID:23980030 - A novel factor Iss10 regulates Mmi1-mediated selective elimination of meiotic transcripts.
Yamashita A et al. Nucleic Acids Res 2013 Nov;41(21):9680-7
PMID:25736293 - Dynactin and Num1 cooperate to establish the cortical anchoring of cytoplasmic dynein in S. pombe.
Fujita I et al. J Cell Sci 2015 Apr 15;128(8):1555-67
PMID:16823445 - Selective elimination of messenger RNA prevents an incidence of untimely meiosis.
Harigaya Y et al. Nature 2006 Jul 06;442(7098):45-50
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:22144463 - RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation.
Zofall M et al. Science 2012 Jan 06;335(6064):96-100
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20622014 - Negative regulation of meiotic gene expression by the nuclear poly(a)-binding protein in fission yeast.
St-André O et al. J Biol Chem 2010 Sep 03;285(36):27859-68
PMID:26644575 - Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast.
Halim A et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15648-53
PMID:15509865 - The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast.
Niccoli T et al. J Cell Sci 2004 Nov 01;117(Pt 23):5543-56
PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.
Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515
PMID:27365210 - The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis.
Marayati BF et al. RNA 2016 Sep;22(9):1349-59
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:32012158 - Meiotic gene silencing complex MTREC/NURS recruits the nuclear exosome to YTH-RNA-binding protein Mmi1.
Shichino Y et al. PLoS Genet 2020 Feb;16(2):e1008598
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:33536434 - A scaffold lncRNA shapes the mitosis to meiosis switch.
Andric V et al. Nat Commun 2021 Feb 03;12(1):770
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:9167972 - Microtubule-associated coiled-coil protein Ssm4 is involved in the meiotic development in fission yeast.
Yamashita A et al. Genes Cells 1997 Feb;2(2):155-66
PMID:26942678 - Enhancer of Rudimentary Cooperates with Conserved RNA-Processing Factors to Promote Meiotic mRNA Decay and Facultative Heterochromatin Assembly.
Sugiyama T et al. Mol Cell 2016 Mar 03;61(5):747-759
PMID:9778252 - RNA-assisted nuclear transport of the meiotic regulator Mei2p in fission yeast.
Yamashita A et al. Cell 1998 Oct 02;95(1):115-23