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protein coding gene - mak2 (SPAC27E2.09) - phosphorelay sensor kinase Mak2

Gene summary

Standard name
mak2
Systematic ID
SPAC27E2.09
Product
phosphorelay sensor kinase Mak2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
phk1
UniProt ID
O14002
ORFeome ID
34/34C08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4027019..4034337 forward strand

Annotation

Complementation

PBO:0091520 - mak1/mak2/mak3 triple deletion functionally complemented by Candida albicans CHK1

References:

GO biological process

GO:0000160 - phosphorelay signal transduction system

References:

GO:1900745 - positive regulation of p38MAPK cascade

References:

GO:0000302 - response to reactive oxygen species

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GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

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GO:0005886 - plasma membrane

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GO:0009365 - protein histidine kinase complex

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GO:0005634 - nucleus

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GO molecular function

GO:0140442 - peroxide sensor activity

References:

GO:0000155 - phosphorelay sensor kinase activity

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GO:0005515 - protein binding

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GO:0020037 - heme binding

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Modification

MOD:00042 - L-aspartic 4-phosphoric anhydride

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MOD:00046 - O-phospho-L-serine

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MOD:00890 - phosphorylated L-histidine

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Multi-locus phenotype

FYPO:0006184 - altered level of osmotic stress responsive gene mRNA during vegetative growth

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Genotypes:

FYPO:0002446 - decreased protein phosphorylation during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0002172 - increased level of nitrogen starvation gene mRNA during vegetative growth

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Genotypes:

FYPO:0001043 - increased mating efficiency

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Genotypes:

FYPO:0003031 - mating without nitrogen starvation

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Genotypes:

FYPO:0006107 - mating, meiosis, and sporulation without starvation

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Genotypes:

FYPO:0001091 - normal cellular response to heat shock

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Genotypes:

FYPO:0001485 - normal cellular response to oxidative stress

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Genotypes:

FYPO:0000968 - normal cellular response to stress during vegetative growth

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Genotypes:

FYPO:0001281 - normal protein phosphorylation during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0002052 - normal sporulation frequency

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Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

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Genotypes:

FYPO:0000852 - resistance to salt stress

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0001408 - sensitive to heat shock

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Genotypes:

FYPO:0000081 - sensitive to high osmolarity

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0002865 - sensitive to polypeptone

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Genotypes:

FYPO:0000271 - sensitive to salt stress

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Genotypes:

FYPO:0000112 - sensitive to sorbitol

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Genotypes:

FYPO:0001492 - viable elongated vegetative cell

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Genotypes:

FYPO:0000648 - viable small vegetative cell

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Genotypes:

FYPO:0002106 - viable stubby vegetative cell

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Protein features

PBO:0111893 - PAC domain protein

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0002446 - decreased protein phosphorylation during cellular response to hydrogen peroxide

References:

Genotypes:

FYPO:0001116 - decreased RNA level during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

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Genotypes:

FYPO:0001043 - increased mating efficiency

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0001485 - normal cellular response to oxidative stress

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Genotypes:

FYPO:0001788 - normal protein localization to cytoplasm

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Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

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Genotypes:

FYPO:0001266 - normal protein phosphorylation during cellular response to salt stress

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Genotypes:

FYPO:0001246 - normal RNA level during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0009036 - resistance to benzamidine

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000271 - sensitive to salt stress

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00069PkinaseProt_kinase_domPfam
PF00072Response_regSig_transdc_resp-reg_receiverPfam
PF00512HisKAHisK_dim/P_domPfam
PF01590GAFGAFPfam
PF02518HATPase_cHATPase_domPfam
PF13191AAA_16AAA_16Pfam
cd00082HisKAHisK_dim/P_domCDD
cd00130PASPASCDD
cd16922HATPase_EvgS-ArcB-TorS-likeCDD
cd17546REC_hyHK_CKI1_RcsC-likeCDD
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE profiles
PS50109HIS_KINHis_kinase_domPROSITE profiles
PS50110RESPONSE_REGULATORYSig_transdc_resp-reg_receiverPROSITE profiles
SM00065GAFGAFSMART
SM00091PASPASSMART
SM00220S_TKcProt_kinase_domSMART
SM00387HATPase_cHATPase_domSMART
SM00388HisKAHisK_dim/P_domSMART
SM00448RECSig_transdc_resp-reg_receiverSMART
PR00344BCTRLSENSORSig_transdc_His_kin-like_CPRINTS
G3DSA:1.10.287.130:FF:000002CATH-FunFam
G3DSA:1.10.510.10:FF:000579CATH-FunFam
G3DSA:3.30.450.20:FF:000099CATH-FunFam
G3DSA:3.30.565.10:FF:000010CATH-FunFam
G3DSA:3.40.50.2300:FF:000679CATH-FunFam
G3DSA:1.10.287.130CATH-Gene3D
G3DSA:1.10.510.10CATH-Gene3D
G3DSA:3.30.450.20CATH-Gene3D
G3DSA:3.30.450.40GAF-like_dom_sfCATH-Gene3D
G3DSA:3.30.565.10HATPase_C_sfCATH-Gene3D
G3DSA:3.40.50.2300CATH-Gene3D
SSF47384HisK_dim/P_sfSUPERFAMILY
SSF48452TPR-like_helical_dom_sfSUPERFAMILY
SSF52172CheY-like_sfSUPERFAMILY
SSF52540P-loop_NTPaseSUPERFAMILY
SSF55781SUPERFAMILY
SSF55785PAS-like_dom_sfSUPERFAMILY
SSF55874HATPase_C_sfSUPERFAMILY
SSF56112Kinase-like_dom_sfSUPERFAMILY
PTHR437192C_system_His_kinasePANTHER

Orthologs

References / Literature

PMID:20919928 - Two-component mediated peroxide sensing and signal transduction in fission yeast.
Quinn J et al. Antioxid Redox Signal 2011 Jul 01;15(1):153-65
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:11179424 - Peroxide sensors for the fission yeast stress-activated mitogen-activated protein kinase pathway.
Buck V et al. Mol Biol Cell 2001 Feb;12(2):407-19
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:12723602 - Characterization of the Prr1 response regulator with special reference to sexual development in Schizosaccharomyces pombe.
Nakamichi N et al. Biosci Biotechnol Biochem 2003 Mar;67(3):547-55
PMID:17559414 - Functional characterization of the phosphorelay protein Mpr1p from Schizosaccharomyces pombe.
Tan H et al. FEMS Yeast Res 2007 Sep;7(6):912-21
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:27613427 - Stress sensitivity of a fission yeast strain lacking histidine kinases is rescued by the ectopic expression of Chk1 from Candida albicans.
Maksimov V et al. Curr Genet 2017 May;63(2):343-357
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:11758939 - Genetic analysis of the His-to-Asp phosphorelay implicated in mitotic cell cycle control: involvement of histidine-kinase genes of Schizosaccharomyces pombe.
Aoyama K et al. Biosci Biotechnol Biochem 2001 Oct;65(10):2347-52
PMID:12596864 - His-to-Asp phosphorelay circuitry for regulation of sexual development in Schizosaccharomyces pombe.
Nakamichi N et al. Biosci Biotechnol Biochem 2002 Dec;66(12):2663-72
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053