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protein coding gene - nuf2 (SPAC27F1.04c) - Ndc80 complex subunit Nuf2

Gene summary

Standard name
nuf2
Systematic ID
SPAC27F1.04c
Product
Ndc80 complex subunit Nuf2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10173
ORFeome ID
23/23A09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4321520..4323223 reverse strand

Annotation

GO biological process

GO:0051315 - attachment of mitotic spindle microtubules to kinetochore

References:

GO:0140456 - initial meiotic spindle pole body separation

References:

GO:0000073 - initial mitotic spindle pole body separation

References:

GO:0051383 - kinetochore organization

References:

GO:1990571 - meiotic centromere clustering

References:

GO:0051455 - spindle attachment to meiosis I kinetochore

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GO cellular component

GO:0000775 - chromosome, centromeric region

References:

GO:0000779 - condensed chromosome, centromeric region

References:

GO:0000776 - kinetochore

References:

GO:0035974 - meiotic spindle pole body

References:

GO:0031262 - Ndc80 complex

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0140483 - kinetochore adaptor activity

References:

GO:0008017 - microtubule binding

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01149 - sumoylated lysine

References:

Multi-locus phenotype

FYPO:0007080 - abnormal initial meiotic spindle pole body separation in meiosis I

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003566 - delayed onset of mitotic spindle pole body separation

References:

Genotypes:

FYPO:0007858 - mitotic DNA re-replication in absence of mitotic spindle

References:

Genotypes:

FYPO:0004367 - normal mitotic spindle assembly

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Genotypes:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000209 - abnormal attachment of spindle microtubules to kinetochore during meiosis I

References:

Genotypes:

FYPO:0000620 - abnormal cell cycle arrest in mitotic metaphase

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0004318 - abolished mitotic spindle assembly checkpoint

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0004212 - decreased protein localization to kinetochore during meiosis I

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Genotypes:

FYPO:0005215 - decreased protein localization to kinetochore during mitotic M phase

References:

Genotypes:

FYPO:0002902 - decreased protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0002649 - elongated kinetochore during mitosis

References:

Genotypes:

FYPO:0005096 - increased duration of protein localization to mitotic spindle pole body during anaphase

References:

Genotypes:

FYPO:0001861 - increased minichromosome loss upon segregation during vegetative growth

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Genotypes:

FYPO:0000316 - inviable after spore germination

References:

Genotypes:

FYPO:0002280 - inviable after spore germination, single cell division

References:

Genotypes:

FYPO:0002379 - inviable after spore germination, without cell division, with elongated germ tube

References:

Genotypes:

FYPO:0000310 - inviable after spore germination, without cell division, with normal germ tube morphology

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0002360 - normal chromatin silencing at centromere

References:

Genotypes:

FYPO:0003762 - normal mitotic spindle assembly checkpoint

References:

Genotypes:

FYPO:0004396 - normal mitotic spindle elongation

References:

Genotypes:

FYPO:0004314 - normal protein localization to CENP-A containing chromatin

References:

Genotypes:

FYPO:0008025 - normal protein localization to CENP-A containing chromatin during mitosis

References:

Genotypes:

FYPO:0002901 - normal protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0005216 - normal protein localization to mitotic spindle pole body during metaphase

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03800Nuf2Kinetochore_Nuf2_NPfam
PF18595Nuf2_DHR10-likeNuf2_DHR10-likePfam
G3DSA:1.10.418.60Nuf2_N_sfCATH-Gene3D
PTHR21650PANTHER
CoilCOILS

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:15371542 - The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.
Kerres A et al. Mol Biol Cell 2004 Dec;15(12):5255-67
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:23770679 - Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation.
Kakui Y et al. Nat Cell Biol 2013 Jul;15(7):786-96
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:11266451 - The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation.
Wigge PA et al. J Cell Biol 2001 Jan 22;152(2):349-60
PMID:17442892 - The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.
Kerres A et al. Mol Biol Cell 2007 Jul;18(7):2441-54
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:15728720 - Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast.
Asakawa H et al. Mol Biol Cell 2005 May;16(5):2325-38
PMID:19846658 - Bub3p facilitates spindle checkpoint silencing in fission yeast.
Vanoosthuyse V et al. Mol Biol Cell 2009 Dec;20(24):5096-105
PMID:36435910 - Fission yeast Dis1 is an unconventional TOG/XMAP215 that induces microtubule catastrophe to drive chromosome pulling.
Murase Y et al. Commun Biol 2022 Nov 26;5(1):1298
PMID:24477934 - Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.
Heinrich S et al. EMBO Rep 2014 Mar;15(3):291-8
PMID:16079914 - Molecular analysis of kinetochore architecture in fission yeast.
Liu X et al. EMBO J 2005 Aug 17;24(16):2919-30
PMID:11685532 - A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint.
Nabetani A et al. Chromosoma 2001 Sep;110(5):322-34
PMID:22065639 - Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation.
Chen JS et al. J Cell Biol 2011 Nov 14;195(4):583-93
PMID:23427262 - The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.
Tang NH et al. Mol Biol Cell 2013 Apr;24(8):1122-33
PMID:28502666 - Purification and characterisation of the fission yeast Ndc80 complex.
Matsuo Y et al. Protein Expr Purif 2017 Jul;135:61-69
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:15930132 - Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles.
Saitoh S et al. Mol Biol Cell 2005 Aug;16(8):3666-77
PMID:31532702 - Kinetochore-mediated outward force promotes spindle pole separation in fission yeast.
Shirasugi Y et al. Mol Biol Cell 2019 Oct 15;30(22):2802-2813
PMID:20967237 - Fission yeast cells undergo nuclear division in the absence of spindle microtubules.
Castagnetti S et al. PLoS Biol 2010 Oct 12;8(10):e1000512
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:39833200 - Centromere positioning orchestrates telomere bouquet formation and the initiation of meiotic differentiation.
Jiménez-Martín A et al. Nat Commun 2025 Jan 20;16(1):837
PMID:41974504 - Nuclear basket subunits Nup211 and Rsm1 influence RNA 3'-processing and transcription termination in fission yeast.
Bednor L et al. RNA 2026 Apr 13;
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:35970865 - The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.
Hirai H et al. Commun Biol 2022 Aug 15;5(1):818
PMID:16079915 - The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast.
Sanchez-Perez I et al. EMBO J 2005 Aug 17;24(16):2931-43
PMID:17352737 - The fission yeast DASH complex is essential for satisfying the spindle assembly checkpoint induced by defects in the inner-kinetochore proteins.
Kobayashi Y et al. Genes Cells 2007 Mar;12(3):311-28
PMID:26483559 - Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly.
Yang HJ et al. J Cell Biol 2015 Oct 26;211(2):295-308
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:11751918 - The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein.
Taricani L et al. J Biol Chem 2002 Mar 22;277(12):10562-72
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:17627824 - The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation.
Gregan J et al. Curr Biol 2007 Jul 17;17(14):1190-200
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26882497 - Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests.
Zich J et al. PLoS Genet 2016 Feb;12(2):e1005834
PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.
Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44
PMID:12928332 - Distinct centromere domain structures with separate functions demonstrated in live fission yeast cells.
Appelgren H et al. J Cell Sci 2003 Oct 01;116(Pt 19):4035-42
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.
Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84