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protein coding gene - ino80 (SPAC29B12.01) - SNF2 family ATP-dependent chromatin remodeller Ino80

Gene summary

Standard name
ino80
Systematic ID
SPAC29B12.01
Product
SNF2 family ATP-dependent chromatin remodeller Ino80
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC3G6.12
UniProt ID
O14148
ORFeome ID
31/31A05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5403218..5408330 forward strand

Annotation

GO biological process

GO:0034080 - CENP-A containing chromatin assembly

References:

GO:0006338 - chromatin remodeling

References:

GO:0006281 - DNA repair

References:

GO:0045815 - transcription initiation-coupled chromatin remodeling

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0031011 - Ino80 complex

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0140658 - ATP-dependent chromatin remodeler activity

References:

GO:0003677 - DNA binding

References:

GO:0042393 - histone binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

References:

Multi-locus phenotype

FYPO:0003217 - decreased chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0006843 - increased histone H3 localization to chromatin

References:

Genotypes:

FYPO:0006823 - viable small vegetative cell with slow cell growth

References:

Genotypes:

Protein features

PBO:0111747 - DEAD/DEAH box helicase

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0003217 - decreased chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0002386 - decreased protein localization to pericentric heterochromatin at centromere outer repeat region

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002150 - inviable spore population

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00271Helicase_CHelicase_C-likePFAM
PF00176SNF2-rel_domSNF2_NPFAM
PF13892DBINODBINOPFAM
cd18793SF2_C_SNFSNF2/RAD54-like_CCDD
cd18002DEXQc_INO80DEXQc_INO80CDD
PS51192HELICASE_ATP_BIND_1Helicase_ATP-bdPROSITE_PROFILES
PS51413DBINODBINOPROSITE_PROFILES
PS51194HELICASE_CTERHelicase_C-likePROSITE_PROFILES
SM00490helicmild6Helicase_C-likeSMART
SM00487ultradead3Helicase_ATP-bdSMART
G3DSA:3.40.50.300:FF:001269FUNFAM
G3DSA:3.40.50.10810:FF:000022FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.10810SNF2-like_sfGENE3D
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR45685HELICASE SRCAP-RELATEDINO80/SWR1_helicasePANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMOBIDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:39387272 - The DNA Damage Repair Function of Fission Yeast CK1 Involves Targeting Arp8, a Subunit of the INO80 Chromatin Remodeling Complex.
Cullati SN et al. Mol Cell Biol 2024 Oct 10;:1-15
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:33378674 - The INO80 Complex Regulates Epigenetic Inheritance of Heterochromatin.
Shan CM et al. Cell Rep 2020 Dec 29;33(13):108561
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:39747188 - PhpC NF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA production.
Srivastav MK et al. Nat Commun 2025 Jan 02;16(1):268
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:19040720 - Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment.
Shevchenko A et al. Genome Biol 2008;9(11):R167
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:19933844 - Fission yeast Iec1-ino80-mediated nucleosome eviction regulates nucleotide and phosphate metabolism.
Hogan CJ et al. Mol Cell Biol 2010 Feb;30(3):657-74
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000051 - S. pombe keyword mapping
PMID:22144913 - Widespread cotranslational formation of protein complexes.
Duncan CD et al. PLoS Genet 2011 Dec;7(12):e1002398
PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.
Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15
PMID:30134042 - The INO80 complex activates the transcription of S-phase genes in a cell cycle-regulated manner.
Knezevic I et al. FEBS J 2018 Oct;285(20):3870-3881
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:36509793 - Functional crosstalk between the cohesin loader and chromatin remodelers.
Muñoz S et al. Nat Commun 2022 Dec 13;13(1):7698
GO_REF:0000002 - Comments
PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.
Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003
PMID:33468217 - Rbm10 facilitates heterochromatin assembly via the Clr6 HDAC complex.
Weigt M et al. Epigenetics Chromatin 2021 Jan 19;14(1):8
PMID:39096900 - Nucleosome remodeler exclusion by histone deacetylation enforces heterochromatic silencing and epigenetic inheritance.
Sahu RK et al. Mol Cell 2024 Sep 05;84(17):3175-3191.e8
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:37043046 - An essential role for the Ino80 chromatin remodeling complex in regulation of gene expression during cellular quiescence.
Zahedi Y et al. Chromosome Res 2023 Apr 12;31(2):14
PMID:28904333 - The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.
Choi ES et al. Nat Commun 2017 Sep 13;8(1):529
PMID:19111658 - HP1 proteins form distinct complexes and mediate heterochromatic gene silencing by nonoverlapping mechanisms.
Motamedi MR et al. Mol Cell 2008 Dec 26;32(6):778-90