protein coding gene - set2 (SPAC29B12.02c) - histone lysine H3-K36 methyltransferase Set2

Gene summary

Standard name

set2

Systematic ID

SPAC29B12.02c

Product

histone lysine H3-K36 methyltransferase Set2

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

kmt3

UniProt ID

O14026

ORFeome ID

28/28F02

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 5408180..5411065 reverse strand

Annotation

Protein features

Orthologs

• SETD2 (HGNC:18420) Homo sapiens
• set2 (SJAG_02873) Schizosaccharomyces japonicus
• SET2 (YJL168C) Saccharomyces cerevisiae

References / Literature

PMID:27268234 - Both H4K20 mono-methylation and H3K56 acetylation mark transcription-dependent histone turnover in fission yeast.

Yang H et al. Biochem Biophys Res Commun 2016 Aug 05;476(4):515-521

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:24662054 - The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription.

Creamer KM et al. Mol Cell Biol 2014 Jun;34(11):2046-61

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:26205977 - Highly condensed chromatins are formed adjacent to subtelomeric and decondensed silent chromatin in fission yeast.

Matsuda A et al. Nat Commun 2015 Jul 24;6:7753

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:29618061 - Histone deacetylation promotes transcriptional silencing at facultative heterochromatin.

Watts BR et al. Nucleic Acids Res 2018 Jun 20;46(11):5426-5440

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:34532559 - Systematic Quantification of GFP-tagged Protein Foci in Schizosaccharomyces pombe Nuclei.

Lim KK et al. Bio Protoc 2018 Dec 20;8(24):e3117

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:28366642 - Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation.

Shetty A et al. Mol Cell 2017 Apr 06;66(1):77-88.e5

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:30674555 - An essential role for dNTP homeostasis following CDK-induced replication stress.

Pai CC et al. J Cell Sci 2019 Mar 25;132(6)

PMID:23032292 - Chd1 chromatin remodelers maintain nucleosome organization and repress cryptic transcription.

Hennig BP et al. EMBO Rep 2012 Nov 06;13(11):997-1003

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:28903048 - Set2 Methyltransferase Facilitates DNA Replication and Promotes Genotoxic Stress Responses through MBF-Dependent Transcription.

Pai CC et al. Cell Rep 2017 Sep 12;20(11):2693-2705

PMID:35157728 - TOR complex 2 contributes to regulation of gene expression via inhibiting Gcn5 recruitment to subtelomeric and DNA replication stress genes.

Cohen A et al. PLoS Genet 2022 Feb;18(2):e1010061

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.

Maki T et al. PLoS Genet 2018 May;14(5):e1007424

PMID:30793188 - A conserved genetic interaction between Spt6 and Set2 regulates H3K36 methylation.

Gopalakrishnan R et al. Nucleic Acids Res 2019 May 07;47(8):3888-3903

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:27613421 - Transcription-coupled changes to chromatin underpin gene silencing by transcriptional interference.

Ard R et al. Nucleic Acids Res 2016 Dec 15;44(22):10619-10630

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:17434129 - S. pombe LSD1 homologs regulate heterochromatin propagation and euchromatic gene transcription.

Lan F et al. Mol Cell 2007 Apr 13;26(1):89-101

PMID:26792892 - Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast.

Suzuki S et al. Nucleic Acids Res 2016 May 19;44(9):4147-62

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:16087749 - Histone H3 K36 methylation is associated with transcription elongation in Schizosaccharomyces pombe.

Morris SA et al. Eukaryot Cell 2005 Aug;4(8):1446-54

PMID:18216783 - Cell cycle control of centromeric repeat transcription and heterochromatin assembly.

Chen ES et al. Nature 2008 Feb 07;451(7179):734-7

PMID:18252195 - Methylations of histone H3 lysine 9 and lysine 36 are functionally linked to DNA replication checkpoint control in fission yeast.

Kim HS et al. Biochem Biophys Res Commun 2008 Apr 04;368(2):419-25

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:40520116 - Histone deacetylation as a landmark for Sgo2 relocation from centromeres to subtelomeres during interphase.

Osaki M et al. iScience 2025 Jun 20;28(6):112717

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:15798214 - A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.

Kizer KO et al. Mol Cell Biol 2005 Apr;25(8):3305-16

PMID:30773398 - Set7 Is a H3K37 Methyltransferase in Schizosaccharomyces pombe and Is Required for Proper Gametogenesis.

Shen Y et al. Structure 2019 Apr 02;27(4):631-638.e8

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:24909977 - A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice.

Pai CC et al. Nat Commun 2014 Jun 09;5:4091

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.

Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8

PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.

Mak T et al. EMBO J 2021 Aug 16;40(16):e107911

PMID:32496538 - Cdk9 and H2Bub1 signal to Clr6-CII/Rpd3S to suppress aberrant antisense transcription.

Sansó M et al. Nucleic Acids Res 2020 Jul 27;48(13):7154-7168

PMID:17450151 - Distinct roles of HDAC complexes in promoter silencing, antisense suppression and DNA damage protection.

Nicolas E et al. Nat Struct Mol Biol 2007 May;14(5):372-80

PMID:22990236 - Hrp3 controls nucleosome positioning to suppress non-coding transcription in eu- and heterochromatin.

Shim YS et al. EMBO J 2012 Nov 28;31(23):4375-87

PMID:28648780 - The Histone Acetyltransferase Mst2 Protects Active Chromatin from Epigenetic Silencing by Acetylating the Ubiquitin Ligase Brl1.

Flury V et al. Mol Cell 2017 Jul 20;67(2):294-307.e9

PMID:29635344 - Histone H3 lysine 36 methyltransferase mobilizes NER factors to regulate tolerance against alkylation damage in fission yeast.

Lim KK et al. Nucleic Acids Res 2018 Jun 01;46(10):5061-5074

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:26804021 - Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.

Tashiro S et al. Nat Commun 2016 Jan 25;7:10393

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.

Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:28718400 - Histone H3G34R mutation causes replication stress, homologous recombination defects and genomic instability in S. pombe .

Yadav RK et al. Elife 2017 Jul 18;6

PMID:15550243 - Methylation of histone H4 lysine 20 controls recruitment of Crb2 to sites of DNA damage.

Sanders SL et al. Cell 2004 Nov 24;119(5):603-14

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:24583182 - A novel method for purification of the endogenously expressed fission yeast Set2 complex.

Suzuki S et al. Protein Expr Purif 2014 May;97:44-9

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83