protein coding gene - spd1 (SPAC29B12.03) - ribonucleotide reductase (RNR) inhibitor

Gene summary

Standard name

spd1

Systematic ID

SPAC29B12.03

Product

ribonucleotide reductase (RNR) inhibitor

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

Q10585

ORFeome ID

02/02F08

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 5412443..5413219 forward strand

Annotation

Protein features

Orthologs

• spd1 (SJAG_02874) Schizosaccharomyces japonicus
• DIF1 (YLR437C) Saccharomyces cerevisiae

References / Literature

PMID:30154212 - Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription.

Rubio A et al. J Cell Sci 2018 Sep 20;131(18)

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:16317005 - The Schizosaccharomyces pombe replication inhibitor Spd1 regulates ribonucleotide reductase activity and dNTPs by binding to the large Cdc22 subunit.

Håkansson P et al. J Biol Chem 2006 Jan 20;281(3):1778-83

PMID:27098497 - CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection.

Zeng M et al. Nat Commun 2016 Apr 21;7:11364

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:21183410 - Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination.

Vejrup-Hansen R et al. DNA Repair (Amst) 2011 Mar 07;10(3):283-95

PMID:32692737 - CRL4Cdt2 ubiquitin ligase regulates Dna2 and Rad16 (XPF) nucleases by targeting Pxd1 for degradation.

Zhang JM et al. PLoS Genet 2020 Jul;16(7):e1008933

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:21123655 - Break-induced ATR and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast.

Moss J et al. Genes Dev 2010 Dec 01;24(23):2705-16

PMID:41259369 - Hydroxyurea induces an oxidative stress response that triggers ER expansion and cytoplasmic protein aggregation.

Sánchez-Molina A et al. PLoS Biol 2025 Nov 19;23(11):e3003493

PMID:23986475 - Spd1 accumulation causes genome instability independently of ribonucleotide reductase activity but functions to protect the genome when deoxynucleotide pools are elevated.

Fleck O et al. J Cell Sci 2013 Nov 01;126(Pt 21):4985-94

PMID:21215368 - The Cul4-Ddb1(Cdt)² ubiquitin ligase inhibits invasion of a boundary-associated antisilencing factor into heterochromatin.

Braun S et al. Cell 2011 Jan 07;144(1):41-54

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:24623809 - The DNA damage checkpoint pathway promotes extensive resection and nucleotide synthesis to facilitate homologous recombination repair and genome stability in fission yeast.

Blaikley EJ et al. Nucleic Acids Res 2014 May;42(9):5644-56

PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.

Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333

PMID:12695334 - Cop9/signalosome subunits and Pcu4 regulate ribonucleotide reductase by both checkpoint-dependent and -independent mechanisms.

Liu C et al. Genes Dev 2003 May 01;17(9):1130-40

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:22464192 - Ribonucleotide reductase activity is coupled to DNA synthesis via proliferating cell nuclear antigen.

Salguero I et al. Curr Biol 2012 Apr 24;22(8):720-6

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:26918805 - Consequences of abnormal CDK activity in S phase.

Anda S et al. Cell Cycle 2016;15(7):963-73

PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.

Rallis C et al. Sci Rep 2017 Mar 10;7:44257

PMID:28903048 - Set2 Methyltransferase Facilitates DNA Replication and Promotes Genotoxic Stress Responses through MBF-Dependent Transcription.

Pai CC et al. Cell Rep 2017 Sep 12;20(11):2693-2705

PMID:15805471 - Ddb1 controls genome stability and meiosis in fission yeast.

Holmberg C et al. Genes Dev 2005 Apr 01;19(7):853-62

PMID:16252005 - Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase.

Liu C et al. EMBO J 2005 Nov 16;24(22):3940-51

PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.

Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697

PMID:24652833 - Spd2 assists Spd1 in the modulation of ribonucleotide reductase architecture but does not regulate deoxynucleotide pools.

Vejrup-Hansen R et al. J Cell Sci 2014 Jun 01;127(Pt 11):2460-70

PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.

Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62

PMID:28441348 - Deoxynucleoside Salvage in Fission Yeast Allows Rescue of Ribonucleotide Reductase Deficiency but Not Spd1-Mediated Inhibition of Replication.

Fleck O et al. Genes (Basel) 2017 Apr 25;8(5)

PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.

Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650

PMID:14701809 - Ddb1 is required for the proteolysis of the Schizosaccharomyces pombe replication inhibitor Spd1 during S phase and after DNA damage.

Bondar T et al. J Biol Chem 2004 Mar 12;279(11):9937-43

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:8887552 - A novel S phase inhibitor in fission yeast.

Woollard A et al. EMBO J 1996 Sep 02;15(17):4603-12

PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.

Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:35974206 - A context-dependent and disordered ubiquitin-binding motif.

Dreier JE et al. Cell Mol Life Sci 2022 Aug 16;79(9):484

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:26518661 - The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast.

Sadeghi L et al. EMBO Rep 2015 Dec;16(12):1673-87

PMID:38261971 - Checkpoint activation by Spd1: a competition-based system relying on tandem disordered PCNA binding motifs.

Olsen JG et al. Nucleic Acids Res 2024 Jan 23;

PMID:26890608 - A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave.

Gaspa L et al. Cell Cycle 2016;15(5):720-9

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:16407242 - An evolutionarily conserved function of proliferating cell nuclear antigen for Cdt1 degradation by the Cul4-Ddb1 ubiquitin ligase in response to DNA damage.

Hu J et al. J Biol Chem 2006 Feb 17;281(7):3753-6

PMID:24634168 - Proteome-wide search for PP2A substrates in fission yeast.

Bernal M et al. Proteomics 2014 Jun;14(11):1367-80

PMID:11069778 - The Spd1p S phase inhibitor can activate the DNA replication checkpoint pathway in fission yeast.

Borgne A et al. J Cell Sci 2000 Dec;113 Pt 23:4341-50