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protein coding gene - nnf1 (SPAC29E6.04) - MIS12/MIND complex subunit Nnf1

Gene summary

Standard name
nnf1
Systematic ID
SPAC29E6.04
Product
MIS12/MIND complex subunit Nnf1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC30.08
UniProt ID
Q09858
ORFeome ID
03/03G04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4409498..4410391 forward strand

Annotation

GO biological process

GO:0051315 - attachment of mitotic spindle microtubules to kinetochore

References:

GO:0051455 - spindle attachment to meiosis I kinetochore

References:

GO cellular component

GO:0000779 - condensed chromosome, centromeric region

References:

GO:0000776 - kinetochore

References:

GO:0000444 - MIS12/MIND type complex

References:

GO:0005634 - nucleus

References:

GO:0000940 - outer kinetochore

References:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0000310 - inviable after spore germination, without cell division, with normal germ tube morphology

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002273 - inviable vegetative cell with abnormal cell morphology

References:

Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000108 - frameshifted

References:

PBO:0091545 - I:g.4410192delG

References:

PBO:0000129 - sequence error in genomic data

References:

Protein features

IDNameInterPro nameDB name
PF03980Nnf1PMF1/Nnf1Pfam
PTHR15459PMF1/Nnf1PANTHER
PIRSF027153Nnf1pNnf1PIRSF
CoilCOILS

Orthologs

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:24477934 - Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.
Heinrich S et al. EMBO Rep 2014 Mar;15(3):291-8
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:29180432 - Phosphorylation of CENP-C by Aurora B facilitates kinetochore attachment error correction in mitosis.
Zhou X et al. Proc Natl Acad Sci U S A 2017 Dec 12;114(50):E10667-E10676
PMID:16079914 - Molecular analysis of kinetochore architecture in fission yeast.
Liu X et al. EMBO J 2005 Aug 17;24(16):2919-30
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.
Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84
PMID:15502821 - A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1.
Obuse C et al. Nat Cell Biol 2004 Nov;6(11):1135-41
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:17352737 - The fission yeast DASH complex is essential for satisfying the spindle assembly checkpoint induced by defects in the inner-kinetochore proteins.
Kobayashi Y et al. Genes Cells 2007 Mar;12(3):311-28
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:36705602 - Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.
Virant D et al. J Cell Biol 2023 Apr 03;222(4)
PMID:20404563 - High-throughput knockout screen in Schizosaccharomyces pombe identifies a novel gene required for efficient homolog disjunction during meiosis I.
Rumpf C et al. Cell Cycle 2010 May;9(9):1802-8
PMID:26615217 - Bulk Segregant Analysis Reveals the Genetic Basis of a Natural Trait Variation in Fission Yeast.
Hu W et al. Genome Biol Evol 2015 Nov 27;7(12):3496-510
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399