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protein coding gene - hsf1 (SPAC2E12.02) - DNA-binding transcription factor Hsf1

Gene summary

Standard name
hsf1
Systematic ID
SPAC2E12.02
Product
DNA-binding transcription factor Hsf1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
hstf, hsf
UniProt ID
Q02953
ORFeome ID
37/37G06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5059255..5062056 forward strand

Annotation

GO biological process

GO:0034605 - cellular response to heat

References:

GO:0045944 - positive regulation of transcription by RNA polymerase II

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0001228 - DNA-binding transcription activator activity, RNA polymerase II-specific

References:

GO:0000981 - DNA-binding transcription factor activity, RNA polymerase II-specific

References:

GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01149 - sumoylated lysine

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Multi-locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0003150 - decreased NETO

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0003155 - intermittent monopolar cell growth

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Genotypes:

FYPO:0003156 - normal monopolar cell growth

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Genotypes:

FYPO:0007301 - normal protein aggregate center formation

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Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000046 - decreased cell population growth

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000658 - decreased DNA binding

References:

Genotypes:

FYPO:0003150 - decreased NETO

References:

Genotypes:

FYPO:0001586 - decreased protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0002287 - decreased RNA level during cellular response to heat

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0007739 - delayed onset of nucleolar ring disassembly

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0003991 - increased RNA level in stationary phase

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Genotypes:

FYPO:0002875 - increased transcription

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000655 - normal DNA binding

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Genotypes:

FYPO:0001037 - normal growth during cellular response to salt stress

References:

Genotypes:

FYPO:0007740 - normal nucleolar ring assembly

References:

Genotypes:

FYPO:0007301 - normal protein aggregate center formation

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0007815 - normal protein localization to protein aggregate center

References:

Genotypes:

FYPO:0004168 - normal viability in stationary phase during glucose starvation

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Genotypes:

FYPO:0002806 - sensitive to arsenate

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000844 - sensitive to mercaptoethanol

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00447HSF_DNA-bindHSF_DNA-bdPfam
PS00434HSF_DOMAINHSF_DNA-bdPROSITE patterns
SM00415HSFHSF_DNA-bdSMART
PR00056HSFDOMAINHSF_DNA-bdPRINTS
G3DSA:1.10.10.10:FF:000027CATH-FunFam
G3DSA:1.10.10.10WH-like_DNA-bd_sfCATH-Gene3D
SSF46785WH_DNA-bd_sfSUPERFAMILY
PTHR10015PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMobiDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:8423799 - Heat shock factor is required for growth at normal temperatures in the fission yeast Schizosaccharomyces pombe.
Gallo GJ et al. Mol Cell Biol 1993 Feb;13(2):749-61
PMID:17347150 - Interaction between heat shock transcription factors (HSFs) and divergent binding sequences: binding specificities of yeast HSFs and human HSF1.
Sakurai H et al. J Biol Chem 2007 May 04;282(18):13334-41
PMID:23695302 - Functional characterization of fission yeast transcription factors by overexpression analysis.
Vachon L et al. Genetics 2013 Aug;194(4):873-84
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:33386485 - Spatial sequestration of misfolded proteins as an active chaperone-mediated process during heat stress.
Boronat S et al. Curr Genet 2021 Apr;67(2):237-243
PMID:36794724 - Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis.
Garg A et al. Nucleic Acids Res 2023 Apr 24;51(7):3094-3115
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:24768994 - The Schizosaccharomyces pombe Hikeshi/Opi10 protein has similar biochemical functions to its human homolog but acts in different physiological contexts.
Oda Y et al. FEBS Lett 2014 May 21;588(10):1899-905
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:18204818 - Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants.
Chung KS et al. Invest New Drugs 2008 Aug;26(4):299-307
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:33176152 - Acute Heat Stress Leads to Reversible Aggregation of Nuclear Proteins into Nucleolar Rings in Fission Yeast.
Gallardo P et al. Cell Rep 2020 Nov 10;33(6):108377
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24146635 - Hsp70-Hsp40 chaperone complex functions in controlling polarized growth by repressing Hsf1-driven heat stress-associated transcription.
Vjestica A et al. PLoS Genet 2013;9(10):e1003886
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:39473973 - Limiting 20S proteasome assembly leads to unbalanced nucleo-cytoplasmic distribution of 26S/30S proteasomes and chronic proteotoxicity.
Ruiz-Romero G et al. iScience 2024 Nov 15;27(11):111095
PMID:10071222 - Mutations in the Schizosaccharomyces pombe heat shock factor that differentially affect responses to heat and cadmium stress.
Saltsman KA et al. Mol Gen Genet 1999 Feb;261(1):161-9
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:27966061 - UBL/BAG-domain co-chaperones cause cellular stress upon overexpression through constitutive activation of Hsf1.
Poulsen EG et al. Cell Stress Chaperones 2017 Jan;22(1):143-154
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:1986225 - Regulation of heat shock factor in Schizosaccharomyces pombe more closely resembles regulation in mammals than in Saccharomyces cerevisiae.
Gallo GJ et al. Mol Cell Biol 1991 Jan;11(1):281-8
PMID:21072667 - hsf1 (+) extends chronological lifespan through Ecl1 family genes in fission yeast.
Ohtsuka H et al. Mol Genet Genomics 2011 Jan;285(1):67-77