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protein coding gene - mns1 (SPAC2E1P5.01c) - mannosyl-oligosaccharide 1,2-alpha-mannosidase

Gene summary

Standard name
mns1
Systematic ID
SPAC2E1P5.01c
Product
mannosyl-oligosaccharide 1,2-alpha-mannosidase
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAPB1E7.13c
UniProt ID
Q9P7C3
ORFeome ID
50/50H04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3322678..3324854 reverse strand

Annotation

Disease association

MONDO:0013624 - Rafiq syndrome

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GO biological process

GO:0097466 - ubiquitin-dependent glycoprotein ERAD pathway

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005783 - endoplasmic reticulum

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GO:0005789 - endoplasmic reticulum membrane

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GO molecular function

GO:0005509 - calcium ion binding

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GO:0004571 - mannosyl-oligosaccharide 1,2-alpha-mannosidase activity

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Modification

MOD:00689 - disulfide crosslinked residues

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MOD:00006 - N-glycosylated residue

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Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001144 - decreased Man8GlcNAc level

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Genotypes:

FYPO:0001146 - decreased misfolded glycoprotein degradation during vegetative growth

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001143 - normal Man9GlcNAc level

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Genotypes:

FYPO:0009066 - resistance to amorolfine

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0005193 - resistance to torin1

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Genotypes:

FYPO:0000094 - sensitive to benomyl

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0001491 - viable vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01532Glyco_hydro_47Glyco_hydro_47PFAM
PR00747GLYHDRLASE47Glyco_hydro_47PRINTS
SSF48225Seven-hairpin glycosidasesSeven-hairpin_glycosidasesSUPERFAMILY
G3DSA:1.50.10.106hp_glycosidase-like_sfGENE3D
PTHR11742MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATEDGlycosyl_Hydrolase_47PANTHER

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:16079177 - Characterization of Schizosaccharomyces pombe ER alpha-mannosidase: a reevaluation of the role of the enzyme on ER-associated degradation.
Movsichoff F et al. Mol Biol Cell 2005 Oct;16(10):4714-24
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000036 - Manual annotations that require more than one source of functional data to support the assignment of the associated GO term
PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. Yeast 2016 Sep;33(9):507-17