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protein coding gene - vps4 (SPAC2G11.06) - AAA family ATPase Vps4

Gene summary

Standard name
vps4
Systematic ID
SPAC2G11.06
Product
AAA family ATPase Vps4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09803
ORFeome ID
24/24H04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 818003..819739 forward strand

Annotation

Disease association

MONDO:0035819 - cerebellar hypoplasia-intellectual disability-congenital microcephaly-dystonia-anemia-growth retardation syndrome

References:

MONDO:0700387 - dentin dysplasia, type IB

References:

GO biological process

GO:0045324 - late endosome to vacuole transport

References:

GO:0007084 - mitotic nuclear membrane reassembly

References:

GO:0043328 - protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

References:

GO:0007033 - vacuole organization

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GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0005768 - endosome

References:

GO:0005634 - nucleus

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GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00048 - O4'-phospho-L-tyrosine

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Multi-locus phenotype

FYPO:0001972 - abnormal cell separation after cytokinesis resulting in septated cell

References:

Genotypes:

FYPO:0000272 - abolished septum assembly

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Genotypes:

FYPO:0000278 - decreased cell population growth following spore germination

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Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

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Genotypes:

FYPO:0001390 - misoriented septum during vegetative growth

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Genotypes:

FYPO:0003166 - monoseptate vegetative cell with binucleate and anucleate compartments

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Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0001513 - normal mitotic sister chromatid segregation

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Genotypes:

FYPO:0003751 - normal nuclear envelope morphology

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Genotypes:

FYPO:0002085 - normal vegetative cell growth

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Genotypes:

FYPO:0000772 - perforated nuclear envelope

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Genotypes:

Protein features

PBO:0111792 - AAA family ATPase

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001972 - abnormal cell separation after cytokinesis resulting in septated cell

References:

Genotypes:

FYPO:0000805 - abnormal endoplasmic reticulum organization

References:

Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0001791 - abnormal mitotic spindle pole body duplication

References:

Genotypes:

FYPO:0001733 - abnormal mitotic spindle pole body separation

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Genotypes:

FYPO:0000769 - abnormal nuclear envelope morphology during vegetative growth

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Genotypes:

FYPO:0003973 - abnormal nuclear pore localization

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Genotypes:

FYPO:0001894 - abnormal sporulation resulting in formation of ascus with more or fewer than four spores

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Genotypes:

FYPO:0010034 - abolished nuclear pore clustering during cellular response to hydroxyurea

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0000278 - decreased cell population growth following spore germination

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0006018 - decreased nuclear pore density

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Genotypes:

FYPO:0006434 - decreased protein localization to medial cortical node, with protein distributed in cell cortex, during vegetative growth

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Genotypes:

FYPO:0000584 - decreased sporulation frequency

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Genotypes:

FYPO:0001475 - fragmented mitotic spindle pole body

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Genotypes:

FYPO:0006019 - karmellae present

References:

Genotypes:

FYPO:0001390 - misoriented septum during vegetative growth

References:

Genotypes:

FYPO:0003166 - monoseptate vegetative cell with binucleate and anucleate compartments

References:

Genotypes:

FYPO:0000516 - normal nuclear import

References:

Genotypes:

FYPO:0008456 - normal plasma membrane hypoosmotic expansion during vegetative growth

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Genotypes:

FYPO:0000772 - perforated nuclear envelope

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00004AAAATPase_AAA_corePfam
PF04212MITMIT_domPfam
PF09336Vps4_CSpast_Vps4_CPfam
PF17862AAA_lid_3AAA_lid_3Pfam
cd02678MIT_VPS4VPS4_MITCDD
PS00674AAAATPase_AAA_CSPROSITE patterns
SM00382AAAAAA+_ATPaseSMART
SM00745MITMIT_domSMART
G3DSA:1.10.8.60:FF:000015CATH-FunFam
G3DSA:1.20.58.80:FF:000004CATH-FunFam
G3DSA:3.40.50.300:FF:000043CATH-FunFam
G3DSA:1.10.8.60CATH-Gene3D
G3DSA:1.20.58.80CATH-Gene3D
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF116846MIT_dom_sfSUPERFAMILY
SSF52540P-loop_NTPaseSUPERFAMILY
PTHR23074MT-severing_AAA_ATPasePANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

GO_REF:0000002 - Comments
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28242692 - LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells.
Gu M et al. Proc Natl Acad Sci U S A 2017 Mar 14;114(11):E2166-E2175
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:22682253 - Functional repurposing revealed by comparing S. pombe and S. cerevisiae genetic interactions.
Frost A et al. Cell 2012 Jun 08;149(6):1339-52
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:40629316 - Ca 2+ -dependent vesicular and non-vesicular lipid transfer controls hypoosmotic plasma membrane expansion.
Mu B et al. BMC Biol 2025 Jul 09;23(1):207
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:41259369 - Hydroxyurea induces an oxidative stress response that triggers ER expansion and cytoplasmic protein aggregation.
Sánchez-Molina A et al. PLoS Biol 2025 Nov 19;23(11):e3003493
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25422470 - Oscillatory AAA+ ATPase Knk1 constitutes a novel morphogenetic pathway in fission yeast.
Scheffler K et al. Proc Natl Acad Sci U S A 2014 Dec 16;111(50):17899-904
PMID:17951524 - Schizosaccharomyces pombe Sst4p, a conserved Vps27/Hrs homolog, functions downstream of phosphatidylinositol 3-kinase Pik3p to mediate proper spore formation.
Onishi M et al. Eukaryot Cell 2007 Dec;6(12):2343-53
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:34382912 - Anillin/Mid1p interacts with the ESCRT-associated protein Vps4p and mitotic kinases to regulate cytokinesis in fission yeast.
Rezig IM et al. Cell Cycle 2021 Sep;20(18):1845-1860
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:17660439 - Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe.
Iwaki T et al. Microbiology (Reading) 2007 Aug;153(Pt 8):2753-2764
PMID:25356547 - A complex network of interactions between mitotic kinases, phosphatases and ESCRT proteins regulates septation and membrane trafficking in S. pombe.
Bhutta MS et al. PLoS One 2014;9(10):e111789
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36