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protein coding gene - ptc3 (SPAC2G11.07c) - serine/threonine protein phosphatase PP2C catalytic subunit Ptc3

Gene summary

Standard name
ptc3
Systematic ID
SPAC2G11.07c
Product
serine/threonine protein phosphatase PP2C catalytic subunit Ptc3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09173
ORFeome ID
25/25A02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 819768..821784 reverse strand

Annotation

GO biological process

GO:0071470 - cellular response to osmotic stress

References:

GO:1903753 - negative regulation of p38MAPK cascade

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GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0043169 - cation binding

References:

GO:1990439 - MAP kinase serine/threonine phosphatase activity

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GO:0004722 - protein serine/threonine phosphatase activity

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Modification

MOD:00006 - N-glycosylated residue

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MOD:00046 - O-phospho-L-serine

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0007434 - attenuated change in cell size at division upon shift to poor nitrogen source

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Genotypes:

FYPO:0001757 - decreased protein phosphatase activity

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Genotypes:

FYPO:0004173 - decreased protein phosphorylation during cellular response to heat

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Genotypes:

FYPO:0004333 - increased protein phosphorylation during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0002415 - inviable swollen vegetative cell with abnormal cell shape

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Genotypes:

FYPO:0001293 - normal cell wall biogenesis

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Genotypes:

FYPO:0001020 - normal growth on calcium

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000098 - sensitive to calcium

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Genotypes:

FYPO:0002641 - sensitive to micafungin

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Genotypes:

FYPO:0000086 - sensitive to tacrolimus

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Genotypes:

FYPO:0001749 - sensitive to tacrolimus during salt stress

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0000647 - vegetative cell lysis

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Genotypes:

FYPO:0002402 - viable swollen vegetative cell with abnormal cell shape

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0001757 - decreased protein phosphatase activity

References:

Genotypes:

FYPO:0004173 - decreased protein phosphorylation during cellular response to heat

References:

Genotypes:

FYPO:0005134 - decreased protein phosphorylation during cellular response to micafungin

References:

Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

References:

Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0001470 - normal growth on tacrolimus

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Genotypes:

FYPO:0004154 - normal protein phosphorylation during cellular response to heat

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00481PP2CPPM-type_phosphatase-like_domPfam
cd00143PP2CcPPM-type_phosphatase-like_domCDD
PS01032PPM_1PP2C_BSPROSITE patterns
PS51746PPM_2PPM-type_phosphatase-like_domPROSITE profiles
SM00332PP2CcPPM-type_phosphatase-like_domSMART
G3DSA:3.60.40.10:FF:000016CATH-FunFam
G3DSA:3.60.40.10PPM-type-like_dom_sfCATH-Gene3D
SSF81606PPM-type-like_dom_sfSUPERFAMILY
PTHR13832PP2CPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:19625445 - Role for RACK1 orthologue Cpc2 in the modulation of stress response in fission yeast.
Núñez A et al. Mol Biol Cell 2009 Sep;20(18):3996-4009
PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.
Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000002 - Comments
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:22139357 - H₂O₂ stress-specific regulation of S. pombe MAPK Sty1 by mitochondrial protein phosphatase Ptc4.
Di Y et al. EMBO J 2012 Feb 01;31(3):563-75
PMID:9790950 - Isoform-specific phosphorylation of fission yeast type 2C protein phosphatase.
Kobayashi T et al. Biochem Biophys Res Commun 1998 Oct 09;251(1):296-300
PMID:10398679 - Heat-shock-induced activation of stress MAP kinase is regulated by threonine- and tyrosine-specific phosphatases.
Nguyen AN et al. Genes Dev 1999 Jul 01;13(13):1653-63
PMID:9211944 - Protein phosphatase 2C acts independently of stress-activated kinase cascade to regulate the stress response in fission yeast.
Gaits F et al. J Biol Chem 1997 Jul 11;272(28):17873-9
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:25639242 - Nitrogen regulates AMPK to control TORC1 signaling.
Davie E et al. Curr Biol 2015 Feb 16;25(4):445-54
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:7859738 - Counteractive roles of protein phosphatase 2C (PP2C) and a MAP kinase kinase homolog in the osmoregulation of fission yeast.
Shiozaki K et al. EMBO J 1995 Feb 01;14(3):492-502
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:17881729 - Atf1 is a target of the mitogen-activated protein kinase Pmk1 and regulates cell integrity in fission yeast.
Takada H et al. Mol Biol Cell 2007 Dec;18(12):4794-802
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10