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protein coding gene - tgs1 (SPAC2G11.15c) - snRNA and snoRNA trimethylguanosine synthase Tgs1

Gene summary

Standard name
tgs1
Systematic ID
SPAC2G11.15c
Product
snRNA and snoRNA trimethylguanosine synthase Tgs1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC521.01c, tgs2
UniProt ID
Q09814
ORFeome ID
12/12G08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 841471..842625 reverse strand

Annotation

GO biological process

GO:0036261 - 7-methylguanosine cap hypermethylation

References:

GO:0033621 - nuclear mRNA surveillance of meiosis-specific transcripts

References:

GO:0140727 - siRNA-mediated pericentric heterochromatin formation

References:

GO:1990273 - snRNA 2,2,7-trimethylguanosine (TMG) capping

References:

GO cellular component

GO:0015030 - Cajal body

References:

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

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GO:0072686 - mitotic spindle

References:

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0071164 - RNA cap trimethylguanosine synthase activity

References:

Modification

MOD:01149 - sumoylated lysine

References:

Multi-locus phenotype

FYPO:0000583 - abolished sporulation

References:

Genotypes:

FYPO:0004201 - decreased centromeric outer repeat transcript-derived siRNA level

References:

Genotypes:

FYPO:0007009 - decreased heterochromatin assembly by small RNA

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000220 - increased centromeric outer repeat transcript level

References:

Genotypes:

FYPO:0002960 - increased level of DSR-containing meiotic gene mRNA during vegetative growth

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001173 - abolished 7-methylguanosine cap hypermethylation

References:

Genotypes:

FYPO:0004201 - decreased centromeric outer repeat transcript-derived siRNA level

References:

Genotypes:

FYPO:0007009 - decreased heterochromatin assembly by small RNA

References:

Genotypes:

FYPO:0003103 - decreased mRNA-derived small RNA level

References:

Genotypes:

FYPO:0002134 - decreased protein-RNA interaction

References:

Genotypes:

FYPO:0010086 - decreased RNA cap trimethylguanosine synthase activity

References:

Genotypes:

FYPO:0008424 - decreased silent mating-type cassette transcript-derived siRNA level

References:

Genotypes:

FYPO:0010098 - decreased subtelomeric transcript-derived siRNA level

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002960 - increased level of DSR-containing meiotic gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0003237 - increased RNA splicing at cryptic splice sites

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009070 - resistance to itraconazole

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0005440 - swollen elongated cell with enlarged nucleus

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF09445Methyltransf_15RNA_cap_Gua-N2-MeTrfasePfam
cd02440AdoMet_MTasesCDD
G3DSA:3.40.50.150:FF:000270CATH-FunFam
G3DSA:3.40.50.150SAM-dependent_MTases_sfCATH-Gene3D
SSF53335SAM-dependent_MTases_sfSUPERFAMILY
PTHR14741PANTHER

Orthologs

References / Literature

PMID:17284461 - Biochemical and genetic analysis of RNA cap guanine-N2 methyltransferases from Giardia lamblia and Schizosaccharomyces pombe.
Hausmann S et al. Nucleic Acids Res 2007;35(5):1411-20
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:27388936 - Disruption of snRNP biogenesis factors Tgs1 and pICln induces phenotypes that mirror aspects of SMN-Gemins complex perturbation in Drosophila, providing new insights into spinal muscular atrophy.
Borg RM et al. Neurobiol Dis 2016 Oct;94:245-58
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:38828770 - The fission yeast ortholog of Coilin, Mug174, forms Cajal body-like nuclear condensates and is essential for cellular quiescence.
Deng X et al. Nucleic Acids Res 2024 Jun 03;
PMID:42420319 - Spliceosomal proteins direct RNA methylation to modulate gene expression and silence retrotransposons.
Vijayakumari D et al. Nat Commun 2026 Jul 08;
PMID:15590684 - Specificity and mechanism of RNA cap guanine-N2 methyltransferase (Tgs1).
Hausmann S et al. J Biol Chem 2005 Feb 11;280(6):4021-4
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24210919 - Mtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance.
Lee NN et al. Cell 2013 Nov 21;155(5):1061-74