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protein coding gene - abc4 (SPAC30.04c) - vacuolar phytochelatin and glutathione S-conjugate ABC family transmembrane transporter Abc4

Gene summary

Standard name
abc4
Systematic ID
SPAC30.04c
Product
vacuolar phytochelatin and glutathione S-conjugate ABC family transmembrane transporter Abc4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9P7V2
ORFeome ID
30/30H01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4395798..4400441 reverse strand

Annotation

Disease association

MONDO:0013768 - arterial calcification, generalized, of infancy, 2

References:

MONDO:0009925 - autosomal recessive inherited pseudoxanthoma elasticum

References:

MONDO:0009380 - Dubin-Johnson syndrome

References:

MONDO:0030058 - hearing loss, autosomal dominant 77

References:

MONDO:0008333 - pseudoxanthoma elasticum, forme fruste

References:

GO biological process

GO:0071996 - glutathione transmembrane import into vacuole

References:

GO:0036246 - phytochelatin 2 import into vacuole

References:

GO:0042908 - xenobiotic transport

References:

GO cellular component

GO:0000324 - fungal-type vacuole

References:

GO:0005774 - vacuolar membrane

References:

GO molecular function

GO:0008559 - ABC-type xenobiotic transporter activity

References:

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0004911 - abolished glutathione import into vacuole

References:

Genotypes:

FYPO:0002829 - decreased cellular phytochelatin level

References:

Genotypes:

FYPO:0002653 - decreased vacuolar import

References:

Genotypes:

FYPO:0004247 - normal vacuolar morphology during vegetative growth

References:

Genotypes:

FYPO:0004912 - phytochelatin absent from vacuole

References:

Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111874 - ABC transporter family

References:

PBO:0111875 - MDR subfamily

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

PomGeneEx:0000013 - RNA level unchanged

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0003849 - normal growth on fluconazole

References:

Genotypes:

FYPO:0003852 - normal growth on FR901464

References:

Genotypes:

FYPO:0000979 - normal growth on miconazole

References:

Genotypes:

FYPO:0003851 - normal growth on MTT

References:

Genotypes:

FYPO:0003850 - normal growth on oligomycin

References:

Genotypes:

FYPO:0002620 - normal growth on trichostatin A

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0002237 - sensitive to cerulenin

References:

Genotypes:

FYPO:0003718 - sensitive to tributyltin

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00005ABC_tranABC_transporter-like_ATP-bdPfam
PF00664ABC_membraneABC1_TM_domPfam
cd03244ABCC_MRP_domain2CDD
cd03250ABCC_MRP_domain1CDD
cd18596ABC_6TM_VMR1_D1_likeCDD
cd18604ABC_6TM_VMR1_D2_likeCDD
PS00211ABC_TRANSPORTER_1ABC_transporter-like_CSPROSITE patterns
PS50893ABC_TRANSPORTER_2ABC_transporter-like_ATP-bdPROSITE profiles
PS50929ABC_TM1FABC1_TM_domPROSITE profiles
SM00382AAAAAA+_ATPaseSMART
G3DSA:1.20.1560.10:FF:000013CATH-FunFam
G3DSA:1.20.1560.10:FF:000220CATH-FunFam
G3DSA:3.40.50.300:FF:000838CATH-FunFam
G3DSA:1.20.1560.10ABC1_TM_sfCATH-Gene3D
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF52540P-loop_NTPaseSUPERFAMILY
SSF90123ABC1_TM_sfSUPERFAMILY
PTHR24223ABC_transporter_C-likePANTHER
Transmembrane alpha helixDeepTMHMM

Orthologs

References / Literature

GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:16849797 - A survey of all 11 ABC transporters in fission yeast: two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant.
Iwaki T et al. Microbiology (Reading) 2006 Aug;152(Pt 8):2309-2321
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21297349 - Bfr1p is responsible for tributyltin resistance in Schizosaccharomyces pombe.
Akiyama K et al. J Toxicol Sci 2011 Jan;36(1):117-20
PMID:19915076 - abc3+ encodes an iron-regulated vacuolar ABC-type transporter in Schizosaccharomyces pombe.
Pouliot B et al. Eukaryot Cell 2010 Jan;9(1):59-73
PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.
Liu NN et al. Cell Res 2010 Aug;20(8):963-5
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:20937798 - Tonoplast-localized Abc2 transporter mediates phytochelatin accumulation in vacuoles and confers cadmium tolerance.
Mendoza-Cózatl DG et al. J Biol Chem 2010 Dec 24;285(52):40416-26
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
GO_REF:0000108 - Automatic assignment of GO terms using logical inference, based on on inter-ontology links.
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
GO_REF:0000002 - Comments
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)