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protein coding gene - hus5 (SPAC30D11.13) - SUMO conjugating enzyme E2 Hus5/Ubc9

Gene summary

Standard name
hus5
Systematic ID
SPAC30D11.13
Product
SUMO conjugating enzyme E2 Hus5/Ubc9
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ubc9
UniProt ID
P40984
ORFeome ID
12/12B08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1094262..1095474 reverse strand

Annotation

GO biological process

GO:0006281 - DNA repair

References:

GO:0016925 - protein sumoylation

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0000792 - heterochromatin

References:

GO:0005634 - nucleus

References:

GO:0106068 - SUMO ligase complex

References:

GO molecular function

GO:0016887 - ATP hydrolysis activity

References:

GO:0005515 - protein binding

References:

GO:0019948 - SUMO activating enzyme activity

References:

GO:0061656 - SUMO conjugating enzyme activity

References:

Modification

MOD:01149 - sumoylated lysine

References:

Multi-locus phenotype

FYPO:0001052 - cut, small cell

References:

Genotypes:

FYPO:0002775 - decreased level of sumoylated protein in cell

References:

Genotypes:

FYPO:0002776 - normal level of sumoylated protein in cell

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000608 - abnormal cell cycle arrest in mitotic M phase

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

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Genotypes:

FYPO:0003209 - abolished protein localization to cell tip, with protein mislocalized to cytoplasm

References:

Genotypes:

FYPO:0002779 - abolished protein localization to nucleus, with protein mislocalized to cytoplasm, during vegetative growth

References:

Genotypes:

FYPO:0005934 - abolished protein sumoylation during vegetative growth

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

References:

Genotypes:

FYPO:0009075 - decreased cell population growth on fructose carbon source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0002775 - decreased level of sumoylated protein in cell

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0002778 - decreased protein sumoylation during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0002262 - inviable after spore germination, multiple cell divisions, elongated cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0000283 - mitotic chromosome fragmentation upon segregation

References:

Genotypes:

FYPO:0002619 - normal growth on sodium butyrate

References:

Genotypes:

FYPO:0002620 - normal growth on trichostatin A

References:

Genotypes:

FYPO:0002776 - normal level of sumoylated protein in cell

References:

Genotypes:

FYPO:0001706 - normal mitotic DNA damage checkpoint during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0000093 - sensitive to arsenic

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000086 - sensitive to tacrolimus

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0000281 - small vacuoles present in increased numbers during vegetative growth

References:

Genotypes:

FYPO:0000024 - stubby vegetative cell

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00179UQ_conUBCPFAM
cd23798UBCc_UBE2ICDD
PS00183UBC_1UBQ-conjugating_ASPROSITE_PATTERNS
PS50127UBC_2UBCPROSITE_PROFILES
SM00212ubc_7SMART
G3DSA:3.10.110.10:FF:000035FUNFAM
SSF54495UBC-likeUBQ-conjugating_enzyme/RWDSUPERFAMILY
G3DSA:3.10.110.10Ubiquitin Conjugating EnzymeUBQ-conjugating_enzyme/RWDGENE3D
PTHR24067UBIQUITIN-CONJUGATING ENZYME E2Ub_conjugating_enzyme-E2-likePANTHER

Orthologs

References / Literature

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:19363481 - Molecular mimicry of SUMO promotes DNA repair.
Prudden J et al. Nat Struct Mol Biol 2009 May;16(5):509-16
PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.
Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333
PMID:16168376 - SUMO modification is involved in the maintenance of heterochromatin stability in fission yeast.
Shin JA et al. Mol Cell 2005 Sep 16;19(6):817-28
PMID:31072933 - Suppressor screening reveals common kleisin-hinge interaction in condensin and cohesin, but different modes of regulation.
Xu X et al. Proc Natl Acad Sci U S A 2019 May 28;116(22):10889-10898
PMID:34853311 - Structural basis for the E3 ligase activity enhancement of yeast Nse2 by SUMO-interacting motifs.
Varejão N et al. Nat Commun 2021 Dec 01;12(1):7013
PMID:15616156 - Tfg3, a subunit of the general transcription factor TFIIF in Schizosaccharomyces pombe, functions under stress conditions.
Kimura M et al. Nucleic Acids Res 2004;32(22):6706-15
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:1427071 - Fission yeast genes involved in coupling mitosis to completion of DNA replication.
Enoch T et al. Genes Dev 1992 Nov;6(11):2035-46
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:8846774 - Fission yeast rad17: a homologue of budding yeast RAD24 that shares regions of sequence similarity with DNA polymerase accessory proteins.
Griffiths DJ et al. EMBO J 1995 Dec 01;14(23):5812-23
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:9092625 - Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance.
Shayeghi M et al. Nucleic Acids Res 1997 Mar 15;25(6):1162-9
PMID:12597774 - Characterization of SUMO-conjugating enzyme mutants in Schizosaccharomyces pombe identifies a dominant-negative allele that severely reduces SUMO conjugation.
Ho JC et al. Biochem J 2003 May 15;372(Pt 1):97-104
PMID:15359282 - Role of the fission yeast SUMO E3 ligase Pli1p in centromere and telomere maintenance.
Xhemalce B et al. EMBO J 2004 Oct 01;23(19):3844-53
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28552615 - SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage.
Wei Y et al. Mol Cell 2017 Jun 01;66(5):581-596.e6
PMID:7768995 - The Schizosaccharomyces pombe hus5 gene encodes a ubiquitin conjugating enzyme required for normal mitosis.
al-Khodairy F et al. J Cell Sci 1995 Feb;108 ( Pt 2):475-86
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:23861937 - Genome-wide screening for genes associated with valproic acid sensitivity in fission yeast.
Zhang L et al. PLoS One 2013;8(7):e68738
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21444718 - DNA repair and global sumoylation are regulated by distinct Ubc9 noncovalent complexes.
Prudden J et al. Mol Cell Biol 2011 Jun;31(11):2299-310
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21850271 - Genome-wide screening for genes associated with FK506 sensitivity in fission yeast.
Ma Y et al. PLoS One 2011;6(8):e23422
PMID:29162938 - RNA metabolism is the primary target of formamide in vivo.
Hoyos-Manchado R et al. Sci Rep 2017 Nov 21;7(1):15895
PMID:11600706 - SUMO modification of Rad22, the Schizosaccharomyces pombe homologue of the recombination protein Rad52.
Ho JC et al. Nucleic Acids Res 2001 Oct 15;29(20):4179-86
PMID:8610150 - Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes.
Kovalenko OV et al. Proc Natl Acad Sci U S A 1996 Apr 02;93(7):2958-63
PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.
Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9