protein coding gene - mis4 (SPAC31A2.05c) - cohesin loading factor (adherin) Mis4/Scc2

Gene summary

Standard name

mis4

Systematic ID

SPAC31A2.05c

Product

cohesin loading factor (adherin) Mis4/Scc2

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

scc2

UniProt ID

Q09725

ORFeome ID

31/31B01

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 391628..396939 reverse strand

Annotation

Protein features

Orthologs

• NIPBL (HGNC:28862) Homo sapiens
• mis4 (SJAG_00796) Schizosaccharomyces japonicus
• SCC2 (YDR180W) Saccharomyces cerevisiae

References / Literature

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.

Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003

PMID:26687354 - DNA Entry into and Exit out of the Cohesin Ring by an Interlocking Gate Mechanism.

Murayama Y et al. Cell 2015 Dec 17;163(7):1628-40

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:29735656 - Suppressor mutation analysis combined with 3D modeling explains cohesin's capacity to hold and release DNA.

Xu X et al. Proc Natl Acad Sci U S A 2018 May 22;115(21):E4833-E4842

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:11882285 - Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast.

Toyoda Y et al. Curr Biol 2002 Mar 05;12(5):347-58

PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.

Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:11598020 - Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism.

Tanaka K et al. EMBO J 2001 Oct 15;20(20):5779-90

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:11554922 - Bir1/Cut17 moving from chromosome to spindle upon the loss of cohesion is required for condensation, spindle elongation and repair.

Morishita J et al. Genes Cells 2001 Sep;6(9):743-63

PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.

Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82

PMID:38830897 - Sororin is an evolutionary conserved antagonist of WAPL.

Prusén Mota I et al. Nat Commun 2024 Jun 03;15(1):4729

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:26583750 - The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering.

Çamdere G et al. Elife 2015 Nov 19;4

PMID:9808627 - Faithful anaphase is ensured by Mis4, a sister chromatid cohesion molecule required in S phase and not destroyed in G1 phase.

Furuya K et al. Genes Dev 1998 Nov 01;12(21):3408-18

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:11069892 - Characterization of fission yeast cohesin: essential anaphase proteolysis of Rad21 phosphorylated in the S phase.

Tomonaga T et al. Genes Dev 2000 Nov 01;14(21):2757-70

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:11861765 - Fission yeast Pds5 is required for accurate chromosome segregation and for survival after DNA damage or metaphase arrest.

Wang SW et al. J Cell Sci 2002 Feb 01;115(Pt 3):587-98

PMID:10779336 - Fission yeast Eso1p is required for establishing sister chromatid cohesion during S phase.

Tanaka K et al. Mol Cell Biol 2000 May;20(10):3459-69

PMID:34309513 - A Brownian ratchet model for DNA loop extrusion by the cohesin complex.

Higashi TL et al. Elife 2021 Jul 26;10

PMID:31072933 - Suppressor screening reveals common kleisin-hinge interaction in condensin and cohesin, but different modes of regulation.

Xu X et al. Proc Natl Acad Sci U S A 2019 May 28;116(22):10889-10898

PMID:19366728 - Genetic control of cellular quiescence in S. pombe.

Sajiki K et al. J Cell Sci 2009 May 01;122(Pt 9):1418-29

PMID:7865880 - Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality.

Takahashi K et al. Mol Biol Cell 1994 Oct;5(10):1145-58

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:28438891 - A second Wpl1 anti-cohesion pathway requires dephosphorylation of fission yeast kleisin Rad21 by PP4.

Birot A et al. EMBO J 2017 May 15;36(10):1364-1378

PMID:32755595 - A Structure-Based Mechanism for DNA Entry into the Cohesin Ring.

Higashi TL et al. Mol Cell 2020 Sep 17;79(6):917-933.e9

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:21189291 - Role for cohesin in the formation of a heterochromatic domain at fission yeast subtelomeres.

Dheur S et al. Mol Cell Biol 2011 Mar;31(5):1088-97

PMID:32277274 - Conserved roles of chromatin remodellers in cohesin loading onto chromatin.

Muñoz S et al. Curr Genet 2020 Oct;66(5):951-956

PMID:19454013 - Conserved features of cohesin binding along fission yeast chromosomes.

Schmidt CK et al. Genome Biol 2009;10(5):R52

PMID:39916665 - In quiescent G0 phase, Schizosaccharomyces pombe Mis4 ensures full nuclear separation during the subsequent M phase.

Suma M et al. J Cell Sci 2025 Feb 07;

PMID:21300781 - Psm3 acetylation on conserved lysine residues is dispensable for viability in fission yeast but contributes to Eso1-mediated sister chromatid cohesion by antagonizing Wpl1.

Feytout A et al. Mol Cell Biol 2011 Apr;31(8):1771-86

PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.

Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643

PMID:24291789 - Biochemical reconstitution of topological DNA binding by the cohesin ring.

Murayama Y et al. Nature 2014 Jan 16;505(7483):367-71

PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.

Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:22144463 - RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation.

Zofall M et al. Science 2012 Jan 06;335(6064):96-100

PMID:33176147 - DNA Binding by the Mis4 Scc2 Loader Promotes Topological DNA Entrapment by the Cohesin Ring.

Kurokawa Y et al. Cell Rep 2020 Nov 10;33(6):108357

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:31895039 - The CDK Pef1 and protein phosphatase 4 oppose each other for regulating cohesin binding to fission yeast chromosomes.

Birot A et al. Elife 2020 Jan 02;9

PMID:18414064 - Schizosaccharomyces pombe Orc5 plays multiple roles in the maintenance of genome stability throughout the cell cycle.

Kato H et al. Cell Cycle 2008 Apr 15;7(8):1085-96

PMID:10398680 - Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation.

Goshima G et al. Genes Dev 1999 Jul 01;13(13):1664-77

PMID:18079700 - Cell-cycle regulation of cohesin stability along fission yeast chromosomes.

Bernard P et al. EMBO J 2008 Jan 09;27(1):111-21

PMID:29358048 - Establishment of DNA-DNA Interactions by the Cohesin Ring.

Murayama Y et al. Cell 2018 Jan 25;172(3):465-477.e15

PMID:18354085 - Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin.

Kimata Y et al. J Cell Sci 2008 Apr 01;121(Pt 7):1107-18

PMID:38448160 - Phosphorylation of Rec8 cohesin complexes regulates mono-orientation of kinetochores in meiosis I.

Liu Y et al. Life Sci Alliance 2024 May;7(5)

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:16682348 - A screen for cohesion mutants uncovers Ssl3, the fission yeast counterpart of the cohesin loading factor Scc4.

Bernard P et al. Curr Biol 2006 May 09;16(9):875-81

PMID:40193710 - An enzymatic-independent function of palmitoyl hydrolase in cohesin loading onto chromosome.

Wang YT et al. Nucleic Acids Res 2025 Mar 20;53(6)

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514