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protein coding gene - gef2 (SPAC31A2.16) - RhoGEF Gef2

Gene summary

Standard name
gef2
Systematic ID
SPAC31A2.16
Product
RhoGEF Gef2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09733
ORFeome ID
48/48C09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 419942..423991 forward strand

Annotation

GO biological process

GO:0035556 - intracellular signal transduction

References:

GO:1902412 - regulation of mitotic cytokinesis

References:

GO cellular component

GO:0032153 - cell division site

References:

GO:0005829 - cytosol

References:

GO:0071341 - medial cortical node

References:

GO:0110085 - mitotic actomyosin contractile ring

References:

GO:0120105 - mitotic actomyosin contractile ring, intermediate layer

References:

GO:0044732 - mitotic spindle pole body

References:

GO molecular function

GO:0005085 - guanyl-nucleotide exchange factor activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

Multi-locus phenotype

FYPO:0000164 - abnormal cell separation after cytokinesis

References:

Genotypes:

FYPO:0002699 - decreased protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0004653 - delayed onset of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0002562 - delayed onset of protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

References:

Genotypes:

FYPO:0004646 - normal duration of mitotic anaphase

References:

Genotypes:

FYPO:0005905 - normal onset of actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0001402 - normal protein localization to cell cortex during vegetative growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001371 - abolished protein localization to medial cortex

References:

Genotypes:

FYPO:0002555 - abolished protein localization to medial cortex during vegetative growth

References:

Genotypes:

FYPO:0003919 - abolished protein localization to medial cortical node

References:

Genotypes:

FYPO:0002026 - actomyosin contractile ring displaced from midpoint

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0002699 - decreased protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0002869 - decreased protein localization to cell division site

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0004653 - delayed onset of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0002562 - delayed onset of protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0003946 - delayed onset of protein localization to cell division site

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0000636 - increased cell population growth rate

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

References:

Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

References:

Genotypes:

FYPO:0004295 - multiseptate cell

References:

Genotypes:

FYPO:0004646 - normal duration of mitotic anaphase

References:

Genotypes:

FYPO:0005905 - normal onset of actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0002559 - normal protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0002558 - normal protein localization to medial cortex during vegetative growth

References:

Genotypes:

FYPO:0002999 - normal protein localization to medial cortical node

References:

Genotypes:

FYPO:0000784 - protein mislocalized to nucleus during vegetative growth

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00621RhoGEFDH_domPFAM
PF17114Nod1Gef2/Nod1_domPFAM
PS00741DH_1GDS_CDC24_CSPROSITE_PATTERNS
PS50010DH_2DH_domPROSITE_PROFILES
SM00325RhoGEF_3DH_domSMART
SSF48065DBL homology domain (DH-domain)DBL_dom_sfSUPERFAMILY
G3DSA:1.20.900.10Dbl homology (DH) domainDBL_dom_sfGENE3D
PTHR12673FACIOGENITAL DYSPLASIA PROTEINFYVE_RhoGEF_PHPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity

Orthologs

References / Literature

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:24947517 - The putative exchange factor Gef3p interacts with Rho3p GTPase and the septin ring during cytokinesis in fission yeast.
Muñoz S et al. J Biol Chem 2014 Aug 08;289(32):21995-2007
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:23149940 - Blt1 and Mid1 provide overlapping membrane anchors to position the division plane in fission yeast.
Guzman-Vendrell M et al. Mol Cell Biol 2013 Jan;33(2):418-28
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:22427686 - Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis.
Lee IJ et al. J Cell Sci 2012 Jun 15;125(Pt 12):2973-85
PMID:30853434 - NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement.
Willet AH et al. Curr Biol 2019 Mar 18;29(6):1055-1063.e2
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:24790095 - Characterization of the roles of Blt1p in fission yeast cytokinesis.
Goss JW et al. Mol Biol Cell 2014 Jul 01;25(13):1946-57
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23349808 - Fission yeast Nod1 is a component of cortical nodes involved in cell size control and division site placement.
Jourdain I et al. PLoS One 2013;8(1):e54142
PMID:28914606 - Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
McDonald NA et al. Elife 2017 Sep 15;6
PMID:31041892 - Fission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesis.
Kwon L et al. BMC Mol Cell Biol 2019 Jan 24;20(1):1
PMID:22298427 - Roles of putative Rho-GEF Gef2 in division-site positioning and contractile-ring function in fission yeast cytokinesis.
Ye Y et al. Mol Biol Cell 2012 Apr;23(7):1181-95
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:23966468 - Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis.
Zhu YH et al. Mol Biol Cell 2013 Oct;24(20):3187-204
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
GO_REF:0000002 - Comments
PMID:25959226 - Mechanistic insights into the anchorage of the contractile ring by anillin and Mid1.
Sun L et al. Dev Cell 2015 May 26;33(4):413-26