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protein coding gene - dni1 (SPAC31G5.07) - tetraspan protein, claudin Dni1

Gene summary

Standard name
dni1
Systematic ID
SPAC31G5.07
Product
tetraspan protein, claudin Dni1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14107
ORFeome ID
05/05D07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2997453..2998157 forward strand

Annotation

GO biological process

GO:0000755 - cytogamy

References:

GO cellular component

GO:1990819 - mating projection actin fusion focus

References:

GO:0043332 - mating projection tip

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GO:0070867 - mating projection tip membrane

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Modification

MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0000584 - decreased sporulation frequency

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Genotypes:

FYPO:0004181 - normal protein localization to cell-cell contact point during mating

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Genotypes:

FYPO:0002052 - normal sporulation frequency

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Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

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SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0000413 - abolished cell fusion during mating

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Genotypes:

FYPO:0006585 - abolished protein localization to shmoo tip membrane, with protein mislocalized to endoplasmic reticulum

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0000470 - decreased mating type switching

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Genotypes:

FYPO:0006587 - decreased protein localization to shmoo tip membrane

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Genotypes:

FYPO:0006589 - decreased protein localization to shmoo tip membrane, with protein distributed in plasma membrane

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Genotypes:

FYPO:0006588 - decreased protein localization to shmoo tip membrane, with protein mislocalized to endoplasmic reticulum

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

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Genotypes:

FYPO:0003136 - excess plasma membrane present

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000636 - increased cell population growth rate

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Genotypes:

FYPO:0001571 - increased protein-protein interaction

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Genotypes:

FYPO:0003135 - normal agglutination

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Genotypes:

FYPO:0003797 - normal conjugation frequency

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Genotypes:

FYPO:0003626 - normal protein localization to shmoo tip

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Genotypes:

FYPO:0000573 - normal shmoo formation

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Genotypes:

FYPO:0004186 - normal vegetative growth on myriocin

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003357 - sensitive to filipin

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Genotypes:

FYPO:0003358 - sensitive to miconazole

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Genotypes:

FYPO:0003356 - sensitive to papuamide B during mating

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Genotypes:

FYPO:0004184 - sensitive to papuamide B during vegetative growth

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF12351Fig1Dni1/Fig1Pfam
PTHR28092Dni1/Fig1PANTHER
Transmembrane alpha helixDeepTMHMM

Orthologs

References / Literature

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24514900 - Membrane organization and cell fusion during mating in fission yeast requires multipass membrane protein Prm1.
Curto MÁ et al. Genetics 2014 Apr;196(4):1059-76
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:41335110 - High-resolution mapping of the actin fusion focus reveals myosin V-dependent formin transport for aster formation.
Thomas V et al. J Cell Biol 2026 Feb 02;225(2)
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.
Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:29134248 - The ancient claudin Dni2 facilitates yeast cell fusion by compartmentalizing Dni1 into a membrane subdomain.
Curto MÁ et al. Cell Mol Life Sci 2018 May;75(9):1687-1706
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:19627505 - The tetraspan protein Dni1p is required for correct membrane organization and cell wall remodelling during mating in Schizosaccharomyces pombe.
Clemente-Ramos JA et al. Mol Microbiol 2009 Aug;73(4):695-709
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603