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protein coding gene - spk1 (SPAC31G5.09c) - MAP kinase Spk1

Gene summary

Standard name
spk1
Systematic ID
SPAC31G5.09c
Product
MAP kinase Spk1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P27638
ORFeome ID
32/32E07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2999546..3001518 reverse strand

Annotation

Complementation

PBO:0017685 - functionally complemented by rat ERK2

References:

PBO:0017684 - functionally complements S. cerevisiae FUS3

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Disease association

MONDO:0033669 - Noonan syndrome 13

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GO biological process

GO:0071507 - pheromone response MAPK cascade

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GO cellular component

GO:0005829 - cytosol

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GO:0044732 - mitotic spindle pole body

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GO:0005634 - nucleus

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GO molecular function

GO:0004707 - MAP kinase activity

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GO:0005515 - protein binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0004481 - abolished cell population growth at high temperature

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Genotypes:

FYPO:0001152 - decreased RNA level during nitrogen starvation

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001384 - abolished protein kinase activity

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Genotypes:

FYPO:0000583 - abolished sporulation

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Genotypes:

FYPO:0003535 - decreased bipolar index

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0000303 - decreased conjugation frequency

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0001152 - decreased RNA level during nitrogen starvation

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Genotypes:

FYPO:0000584 - decreased sporulation frequency

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Genotypes:

FYPO:0003770 - decreased transcriptional response to pheromone

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000637 - increased cell population growth rate on glucose carbon source

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Genotypes:

FYPO:0003532 - increased monopolar index

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0001883 - normal growth on caspofungin

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Genotypes:

FYPO:0001238 - normal growth on staurosporine

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0001266 - normal protein phosphorylation during cellular response to salt stress

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Genotypes:

FYPO:0000767 - resistance to staurosporine

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0003840 - sensitive to carbendazim

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0002167 - sensitive to ethyl methanesulfonate

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0000280 - sterile

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00069PkinaseProt_kinase_domPFAM
cd07849STKc_ERK1_2_likeCDD
PS01351MAPKMAP_kinase_CSPROSITE_PATTERNS
PS00107PROTEIN_KINASE_ATPProtein_kinase_ATP_BSPROSITE_PATTERNS
PS00108PROTEIN_KINASE_STSer/Thr_kinase_ASPROSITE_PATTERNS
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE_PROFILES
SM00220serkin_6Prot_kinase_domSMART
PR01773P38MAPKINASEMAPK_HOG-likePRINTS
G3DSA:1.10.510.10:FF:000040FUNFAM
G3DSA:3.30.200.20:FF:000073FUNFAM
SSF56112Protein kinase-like (PK-like)Kinase-like_dom_sfSUPERFAMILY
G3DSA:3.30.200.20Phosphorylase Kinase; domain 1GENE3D
G3DSA:1.10.510.10Transferase(Phosphotransferase) domain 1GENE3D
PTHR24055MITOGEN-ACTIVATED PROTEIN KINASEMAPKPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:9133664 - The Schizosaccharomyces pombe mra1 gene, which is required for cell growth and mating, can suppress the mating inefficiency caused by a deficit in the Ras1 activity.
Hakuno F et al. Genes Cells 1996 Mar;1(3):303-15
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:8264618 - Concerted action of RAS and G proteins in the sexual response pathways of Schizosaccharomyces pombe.
Xu HP et al. Mol Cell Biol 1994 Jan;14(1):50-8
PMID:15713656 - Constitutive activation of the fission yeast pheromone-responsive pathway induces ectopic meiosis and reveals ste11 as a mitogen-activated protein kinase target.
Kjaerulff S et al. Mol Cell Biol 2005 Mar;25(5):2045-59
PMID:8443406 - Functional homology of protein kinases required for sexual differentiation in Schizosaccharomyces pombe and Saccharomyces cerevisiae suggests a conserved signal transduction module in eukaryotic organisms.
Neiman AM et al. Mol Biol Cell 1993 Jan;4(1):107-20
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:8413241 - Schizosaccharomyces pombe Spk1 is a tyrosine-phosphorylated protein functionally related to Xenopus mitogen-activated protein kinase.
Gotoh Y et al. Mol Cell Biol 1993 Oct;13(10):6427-34
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:25252312 - [Ash2, a subunit of histone H3K4 methyltransferase complex, is involved in the sporulation in Schizosaccharomyces pombe].
Wang W et al. Yi Chuan 2014 Sep;36(9):943-51
PMID:1899230 - Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.
Toda T et al. Genes Dev 1991 Jan;5(1):60-73
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:33711009 - Structure-function analysis of fission yeast cleavage and polyadenylation factor (CPF) subunit Ppn1 and its interactions with Dis2 and Swd22.
Benjamin B et al. PLoS Genet 2021 Mar;17(3):e1009452
PMID:9135147 - Spm1, a stress-activated MAP kinase that regulates morphogenesis in S.pombe.
Zaitsevskaya-Carter T et al. EMBO J 1997 Mar 17;16(6):1318-31
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:9660817 - Cloning and characterization of shk2, a gene encoding a novel p21-activated protein kinase from fission yeast.
Yang P et al. J Biol Chem 1998 Jul 17;273(29):18481-9
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:7769002 - A novel essential fission yeast gene pad1+ positively regulates pap1(+)-dependent transcription and is implicated in the maintenance of chromosome structure.
Shimanuki M et al. J Cell Sci 1995 Feb;108 ( Pt 2):569-79
PMID:11683500 - Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase Byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe.
Henkel J et al. Differentiation 2001 Jun;67(4-5):98-106
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:17032641 - Global roles of Ste11p, cell type, and pheromone in the control of gene expression during early sexual differentiation in fission yeast.
Mata J et al. Proc Natl Acad Sci U S A 2006 Oct 17;103(42):15517-22
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.
Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:8196631 - Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3.
Kjaerulff S et al. Mol Cell Biol 1994 Jun;14(6):3895-905
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23370392 - A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe.
Kovacikova I et al. Cell Cycle 2013 Feb 15;12(4):618-24
PMID:19279143 - Role of the RNA-binding protein Nrd1 and Pmk1 mitogen-activated protein kinase in the regulation of myosin mRNA stability in fission yeast.
Satoh R et al. Mol Biol Cell 2009 May;20(9):2473-85
PMID:19037094 - The Rho1p exchange factor Rgf1p signals upstream from the Pmk1 mitogen-activated protein kinase pathway in fission yeast.
Garcia P et al. Mol Biol Cell 2009 Jan;20(2):721-31
PMID:8943330 - The fission yeast pmk1+ gene encodes a novel mitogen-activated protein kinase homolog which regulates cell integrity and functions coordinately with the protein kinase C pathway.
Toda T et al. Mol Cell Biol 1996 Dec;16(12):6752-64
PMID:24167631 - Klf1, a C2H2 zinc finger-transcription factor, is required for cell wall maintenance during long-term quiescence in differentiated G0 phase.
Shimanuki M et al. PLoS One 2013;8(10):e78545
PMID:22212525 - Chronological lifespan extension by Ecl1 family proteins depends on Prr1 response regulator in fission yeast.
Ohtsuka H et al. Genes Cells 2012 Jan;17(1):39-52
PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.
Materne P et al. Elife 2015 Jun 22;4:e09008
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:14665462 - Sla1, a Schizosaccharomyces pombe homolog of the human La protein, induces ectopic meiosis when its C terminus is truncated.
Tanabe K et al. Eukaryot Cell 2003 Dec;2(6):1274-87
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:32282918 - Genetic interactions and transcriptomics implicate fission yeast CTD prolyl isomerase Pin1 as an agent of RNA 3' processing and transcription termination that functions via its effects on CTD phosphatase Ssu72.
Sanchez AM et al. Nucleic Acids Res 2020 May 21;48(9):4811-4826
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20501954 - Search for kinases related to transition of growth polarity in fission yeast.
Koyano T et al. Biosci Biotechnol Biochem 2010;74(5):1129-33
PMID:32361273 - Requirement of PP2A-B56 Par1 for the Stabilization of the CDK Inhibitor Rum1 and Activation of APC/C Ste9 during Pre-Start G1 in S. pombe.
Stonyte V et al. iScience 2020 May 22;23(5):101063
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813
PMID:7975894 - Mating pheromone-induced expression of the mat1-Pm gene of Schizosaccharomyces pombe: identification of signalling components and characterization of upstream controlling elements.
Aono T et al. Yeast 1994 Jun;10(6):757-70
PMID:8668157 - Schizosaccharomyces pombe map1+ encodes a MADS-box-family protein required for cell-type-specific gene expression.
Yabana N et al. Mol Cell Biol 1996 Jul;16(7):3420-8