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protein coding gene - maf1 (SPAC31G5.12c) - repressor of RNA polymerase III Maf1

Gene summary

Standard name
maf1
Systematic ID
SPAC31G5.12c
Product
repressor of RNA polymerase III Maf1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
n150
UniProt ID
O14109
ORFeome ID
40/40A10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3007584..3009378 reverse strand

Annotation

GO biological process

GO:0016480 - negative regulation of transcription by RNA polymerase III

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GO cellular component

GO:0005634 - nucleus

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GO molecular function

GO:0106250 - DNA-binding transcription repressor activity, RNA polymerase III-specific

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GO:0000994 - RNA polymerase III core binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0005993 - normal cytoplasmic translation

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Genotypes:

FYPO:0007328 - normal number of Rad52 foci during vegetative growth

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Genotypes:

FYPO:0001310 - normal viability in stationary phase

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Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0001797 - abnormal translation

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0006748 - decreased pre-tRNA level during vegetative growth

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Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0001555 - formation of abnormal tRNA processing intermediates

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Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

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Genotypes:

FYPO:0006520 - increased cytoplasmic translation

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Genotypes:

FYPO:0007329 - increased DNA damage at tRNA genes

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Genotypes:

FYPO:0002564 - increased mature tRNA level during vegetative growth

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Genotypes:

FYPO:0000972 - increased number of Rad52 foci during vegetative growth

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Genotypes:

FYPO:0008100 - increased polyadenylated 5S rRNA level during cellular response to phosphate starvation

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Genotypes:

FYPO:0006749 - increased pre-tRNA level during vegetative growth

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Genotypes:

FYPO:0008102 - increased tRNA level during cellular response to phosphate starvation

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0008123 - loss of viability in stationary phase upon phosphate starvation

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0004295 - multiseptate cell

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Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

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Genotypes:

FYPO:0001147 - normal mating efficiency

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Genotypes:

FYPO:0008119 - normal morphology during phosphate starvation

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Genotypes:

FYPO:0007020 - normal tRNA guanosine N2-methylation

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF09174Maf1Maf1PFAM
G3DSA:3.40.1000.50Repressor of RNA polymerase III transcription Maf1Maf1_sfGENE3D
PTHR22504REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1Maf1PANTHER
PIRSF037240MAF1Maf1PIRSF
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMOBIDB-Negative-Polyelectrolyte

Orthologs

References / Literature

PMID:33475456 - Maf1 limits RNA polymerase III-directed transcription to preserve genomic integrity and extend lifespan.
Noguchi C et al. Cell Cycle 2021 Feb;20(3):247-255
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:34499159 - Fission yeast TOR complex 1 phosphorylates Psk1 through an evolutionarily conserved interaction mediated by the TOS motif.
Morozumi Y et al. J Cell Sci 2021 Oct 01;134(19)
PMID:29330317 - tRNA production links nutrient conditions to the onset of sexual differentiation through the TORC1 pathway.
Otsubo Y et al. EMBO Rep 2018 Mar;19(3)
PMID:36794724 - Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis.
Garg A et al. Nucleic Acids Res 2023 Apr 24;51(7):3094-3115
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:15590667 - Two steps in Maf1-dependent repression of transcription by RNA polymerase III.
Desai N et al. J Biol Chem 2005 Feb 25;280(8):6455-62
PMID:27191590 - Fission Yeast SCYL1/2 Homologue Ppk32: A Novel Regulator of TOR Signalling That Governs Survival during Brefeldin A Induced Stress to Protein Trafficking.
Kowalczyk KM et al. PLoS Genet 2016 May;12(5):e1006041
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:31833215 - Maf1-dependent transcriptional regulation of tRNAs prevents genomic instability and is associated with extended lifespan.
Shetty M et al. Aging Cell 2020 Feb;19(2):e13068
PMID:23213482 - TORC2 and the AGC kinase Gad8 regulate phosphorylation of the ribosomal protein S6 in fission yeast.
Du W et al. Biol Open 2012 Sep 15;1(9):884-8
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:37805140 - Crosstalk between the tRNA methyltransferase Trm1 and RNA chaperone La influences eukaryotic tRNA maturation.
Porat J et al. J Biol Chem 2023 Nov;299(11):105326
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:25639242 - Nitrogen regulates AMPK to control TORC1 signaling.
Davie E et al. Curr Biol 2015 Feb 16;25(4):445-54
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.
Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26720005 - RNA Polymerase III Output Is Functionally Linked to tRNA Dimethyl-G26 Modification.
Arimbasseri AG et al. PLoS Genet 2015 Dec;11(12):e1005671
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6