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protein coding gene - wdr70 (SPAC343.17c) - DDB1-CUL4-associated factor (DCAF), Wdr70

Gene summary

Standard name
wdr70
Systematic ID
SPAC343.17c
Product
DDB1-CUL4-associated factor (DCAF), Wdr70
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UT73
ORFeome ID
45/45H03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1673685..1680106 reverse strand

Annotation

GO biological process

GO:0000724 - double-strand break repair via homologous recombination

References:

GO cellular component

GO:0080008 - Cul4-RING E3 ubiquitin ligase complex

References:

GO:0005634 - nucleus

References:

GO:0035861 - site of double-strand break

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0002553 - abnormal double-strand break processing

References:

Genotypes:

FYPO:0003486 - abolished protein localization to double-strand break site

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0003912 - decreased double-strand break repair via homologous recombination

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Genotypes:

FYPO:0005608 - decreased histone H2B-K119 ubiquitination at double-strand break site during vegetative growth

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Genotypes:

FYPO:0002474 - decreased protein localization to double-strand break site

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Genotypes:

FYPO:0005609 - decreased spatial extent of histone H2B-K119 ubiquitination at double-strand break site during vegetative growth

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Genotypes:

FYPO:0005610 - decreased spatial extent of protein localization to double-strand break site

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0005606 - delayed onset of protein localization to double-strand break site

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Genotypes:

FYPO:0003584 - increased double-strand break repair via nonhomologous end joining

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Genotypes:

FYPO:0005604 - increased duration of Rad52 focus presence during cellular response to ionizing radiation

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Genotypes:

FYPO:0005605 - increased duration of Ssb1 focus presence during cellular response to ionizing radiation

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Genotypes:

FYPO:0005611 - increased H4-K20 dimethylation at double-strand break site during vegetative growth

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Genotypes:

FYPO:0002475 - increased protein localization to double-strand break site

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0002554 - normal protein localization to double-strand break site

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

Protein features

PBO:0111751 - WD repeat protein

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000229 - cut

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Genotypes:

FYPO:0003912 - decreased double-strand break repair via homologous recombination

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003490 - increased duration of mitotic DNA damage checkpoint during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0005603 - increased duration of mitotic DNA damage checkpoint during cellular response to methyl methanesulfonate

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Genotypes:

FYPO:0000972 - increased number of Rad52 foci during vegetative growth

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Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0004229 - normal growth during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

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Genotypes:

FYPO:0001690 - normal growth on camptothecin

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Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

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Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00400WD40WD40_rptPFAM
PS50294WD_REPEATS_REGIONPROSITE_PROFILES
PS50082WD_REPEATS_2WD40_rptPROSITE_PROFILES
SM00320WD40_4WD40_rptSMART
PR00320GPROTEINBRPTWD40_PAC1PRINTS
G3DSA:2.130.10.10:FF:002226FUNFAM
SSF50978WD40 repeat-likeWD40_repeat_dom_sfSUPERFAMILY
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfGENE3D
PTHR16017GASTRULATION DEFECTIVE PROTEIN 1-RELATEDWD_repeat_GAD-1PANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMOBIDB-Negative-Polyelectrolyte

Orthologs

References / Literature

PMID:27098497 - CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection.
Zeng M et al. Nat Commun 2016 Apr 21;7:11364
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:21386897 - Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.
Ren L et al. PLoS One 2011 Feb 28;6(2):e16719
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623