protein coding gene - pkl1 (SPAC3A11.14c) - kinesin-14 family minus-end directed microtubule motor Pkl1

Gene summary

Standard name

pkl1

Systematic ID

SPAC3A11.14c

Product

kinesin-14 family minus-end directed microtubule motor Pkl1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

klp1, SPAC3H5.03c

UniProt ID

Q92376

ORFeome ID

49/49F02

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 3438890..3442117 forward strand

Annotation

Protein features

Orthologs

• KIFC1 (HGNC:6389) Homo sapiens
• pkl1 (SJAG_01546) Schizosaccharomyces japonicus
• KAR3 (YPR141C) Saccharomyces cerevisiae

References / Literature

PMID:25987607 - The Msd1-Wdr8-Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies.

Yukawa M et al. J Cell Biol 2015 May 25;209(4):549-62

PMID:29021344 - A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.

Yukawa M et al. Mol Biol Cell 2017 Dec 01;28(25):3647-3659

PMID:17409356 - Mitotic chromosome biorientation in fission yeast is enhanced by dynein and a minus-end-directed, kinesin-like protein.

Grishchuk EL et al. Mol Biol Cell 2007 Jun;18(6):2216-25

PMID:29684553 - Fission yeast cells overproducing HSET/KIFC1 provides a useful tool for identification and evaluation of human kinesin-14 inhibitors.

Yukawa M et al. Fungal Genet Biol 2018 Jul;116:33-41

PMID:24239120 - Antagonistic spindle motors and MAPs regulate metaphase spindle length and chromosome segregation.

Syrovatkina V et al. Curr Biol 2013 Dec 02;23(23):2423-9

PMID:30463883 - Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/Kinesin-5 Motor for Mitotic Spindle Assembly in Schizosaccharomyces pombe .

Yukawa M et al. G3 (Bethesda) 2019 Jan 09;9(1):269-280

PMID:26031557 - Loss of kinesin-14 results in aneuploidy via kinesin-5-dependent microtubule protrusions leading to chromosome cut.

Syrovatkina V et al. Nat Commun 2015 Jun 02;6:7322

PMID:8898367 - Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function.

Pidoux AL et al. Mol Biol Cell 1996 Oct;7(10):1639-55

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:34346498 - Loss of kinesin-8 improves the robustness of the self-assembled spindle in Schizosaccharomyces pombe.

Pineda-Santaella A et al. J Cell Sci 2021 Aug 15;134(16)

PMID:34951983 - Force by minus-end motors Dhc1 and Klp2 collapses the S. pombe spindle after laser ablation.

Zareiesfandabadi P et al. Biophys J 2022 Jan 18;121(2):263-276

PMID:25869666 - Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways.

Scheffler K et al. J Cell Biol 2015 Apr 13;209(1):47-58

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:19838064 - Distinct Kinesin-14 mitotic mechanisms in spindle bipolarity.

Simeonov DR et al. Cell Cycle 2009 Nov 01;8(21):3571-83

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:11967147 - Two kinesin-like Kin I family proteins in fission yeast regulate the establishment of metaphase and the onset of anaphase A.

Garcia MA et al. Curr Biol 2002 Apr 16;12(8):610-21

PMID:29167352 - Two spatially distinct kinesin-14 proteins, Pkl1 and Klp2, generate collaborative inward forces against kinesin-5 Cut7 in S. pombe .

Yukawa M et al. J Cell Sci 2018 Jan 04;131(1)

PMID:32723864 - Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I.

Zheng F et al. J Biol Chem 2020 Sep 18;295(38):13287-13298

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:19686686 - Phospho-regulated interaction between kinesin-6 Klp9p and microtubule bundler Ase1p promotes spindle elongation.

Fu C et al. Dev Cell 2009 Aug;17(2):257-67

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.

Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72

PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.

Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119

PMID:31532702 - Kinetochore-mediated outward force promotes spindle pole separation in fission yeast.

Shirasugi Y et al. Mol Biol Cell 2019 Oct 15;30(22):2802-2813

PMID:16611238 - The carboxy-terminus of Alp4 alters microtubule dynamics to induce oscillatory nuclear movement led by the spindle pole body in Schizosaccharomyces pombe.

Masuda H et al. Genes Cells 2006 Apr;11(4):337-52

PMID:18984586 - Diffusion and directed movement: in vitro motile properties of fission yeast kinesin-14 Pkl1.

Furuta K et al. J Biol Chem 2008 Dec 26;283(52):36465-73

PMID:23388459 - Kinesin-14 Pkl1 targets γ-tubulin for release from the γ-tubulin ring complex (γ-TuRC) ‬‬‬‬‬‬‬.

Olmsted ZT et al. Cell Cycle 2013 Mar 01;12(5):842-8

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

GO_REF:0000002 - Comments

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:16855399 - An extended anaphase signaling pathway for Mad2p includes microtubule organizing center proteins and multiple motor-dependent transitions.

Mayer C et al. Cell Cycle 2006 Jul;5(13):1456-63

PMID:31089172 - Kinesin-6 Klp9 plays motor-dependent and -independent roles in collaboration with Kinesin-5 Cut7 and the microtubule crosslinker Ase1 in fission yeast.

Yukawa M et al. Sci Rep 2019 May 14;9(1):7336

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:10641037 - A fission yeast kinesin affects Golgi membrane recycling.

Brazer SC et al. Yeast 2000 Jan 30;16(2):149-66

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:30806623 - Kinesin-6 regulates cell-size-dependent spindle elongation velocity to keep mitosis duration constant in fission yeast.

Krüger LK et al. Elife 2019 Feb 26;8

PMID:32327557 - Kinesin-14 family proteins and microtubule dynamics define S. pombe mitotic and meiotic spindle assembly, and elongation.

Loncar A et al. J Cell Sci 2020 Jun 08;133(11)

PMID:27697865 - Inositol Pyrophosphate Kinase Asp1 Modulates Chromosome Segregation Fidelity and Spindle Function in Schizosaccharomyces pombe.

Topolski B et al. Mol Cell Biol 2016 Dec 15;36(24):3128-3140

PMID:34080538 - Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.

Krüger LK et al. Elife 2021 Jun 03;10

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:25348260 - Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells.

Olmsted ZT et al. Nat Commun 2014 Oct 28;5:5339

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:28513584 - Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast.

Rincon SA et al. Nat Commun 2017 May 17;8:15286

PMID:10749926 - A mutation in gamma-tubulin alters microtubule dynamics and organization and is synthetically lethal with the kinesin-like protein pkl1p.

Paluh JL et al. Mol Biol Cell 2000 Apr;11(4):1225-39

PMID:23770679 - Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation.

Kakui Y et al. Nat Cell Biol 2013 Jul;15(7):786-96

PMID:17036054 - Microtubule depolymerization can drive poleward chromosome motion in fission yeast.

Grishchuk EL et al. EMBO J 2006 Oct 18;25(20):4888-96

PMID:11018050 - Tea2p is a kinesin-like protein required to generate polarized growth in fission yeast.

Browning H et al. J Cell Biol 2000 Oct 02;151(1):15-28

PMID:11694582 - pkl1(+)and klp2(+): Two kinesins of the Kar3 subfamily in fission yeast perform different functions in both mitosis and meiosis.

Troxell CL et al. Mol Biol Cell 2001 Nov;12(11):3476-88

PMID:18418055 - Protein complexes at the microtubule organizing center regulate bipolar spindle assembly.

Rodriguez AS et al. Cell Cycle 2008 May 01;7(9):1246-53