protein coding gene - cwf2 (SPAC3A12.11c) - zf-CCCH type zinc finger protein, Prp19 complex subunit, RNA-binding Cwf2

Gene summary

Standard name

cwf2

Systematic ID

SPAC3A12.11c

Product

zf-CCCH type zinc finger protein, Prp19 complex subunit, RNA-binding Cwf2

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

prp3, cwc2

UniProt ID

P87126

ORFeome ID

20/20C12

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 1439245..1440558 reverse strand

Annotation

Protein features

Orthologs

• RBM22 (HGNC:25503) Homo sapiens
• cwf2 (SJAG_04898) Schizosaccharomyces japonicus
• CWC2 (YDL209C) Saccharomyces cerevisiae

References / Literature

PMID:11884590 - Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.

Ohi MD et al. Mol Cell Biol 2002 Apr;22(7):2011-24

PMID:15755920 - Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex.

Carnahan RH et al. Eukaryot Cell 2005 Mar;4(3):577-87

PMID:18948543 - Splicing factors facilitate RNAi-directed silencing in fission yeast.

Bayne EH et al. Science 2008 Oct 24;322(5901):602-6

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:24442611 - Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.

Chen W et al. RNA 2014 Mar;20(3):308-20

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

GO_REF:0000002 - Comments

PMID:26292707 - Structure of a yeast spliceosome at 3.6-angstrom resolution.

Yan C et al. Science 2015 Sep 11;349(6253):1182-91

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.

Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:20018856 - Involvement of the spliceosomal U4 small nuclear RNA in heterochromatic gene silencing at fission yeast centromeres.

Chinen M et al. J Biol Chem 2010 Feb 19;285(8):5630-8

PMID:24014766 - Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.

Livesay SB et al. Eukaryot Cell 2013 Nov;12(11):1472-89

PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .

Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)

PMID:39833470 - Structures of aberrant spliceosome intermediates on their way to disassembly.

Soni K et al. Nat Struct Mol Biol 2025 Jan 20;

PMID:22240020 - Characterization of the ptr5+ gene involved in nuclear mRNA export in fission yeast.

Watanabe N et al. Biochem Biophys Res Commun 2012 Feb 03;418(1):62-6

PMID:24874881 - The proper splicing of RNAi factors is critical for pericentric heterochromatin assembly in fission yeast.

Kallgren SP et al. PLoS Genet 2014;10(5):e1004334

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .

Budden AM et al. Elife 2023 Aug 24;12

PMID:12088152 - Characterization of interactions among the Cef1p-Prp19p-associated splicing complex.

Ohi MD et al. RNA 2002 Jun;8(6):798-815

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.

Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701

PMID:9003295 - Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces pombe.

Urushiyama S et al. Mol Gen Genet 1996 Nov 27;253(1-2):118-27

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.

Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101

PMID:31219728 - Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe .

Cipakova I et al. Cell Cycle 2019 Jul;18(14):1532-1536

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:10409726 - Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing.

McDonald WH et al. Mol Cell Biol 1999 Aug;19(8):5352-62

PMID:21386897 - Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.

Ren L et al. PLoS One 2011 Feb 28;6(2):e16719

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:25274039 - A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.

Bayne EH et al. Genome Biol 2014;15(10):481