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protein coding gene - cam1 (SPAC3A12.14) - calmodulin Cam1

Gene summary

Standard name
cam1
Systematic ID
SPAC3A12.14
Product
calmodulin Cam1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P05933
ORFeome ID
06/06C03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1444850..1446333 forward strand

Annotation

Complementation

PBO:0013025 - functionally complemented by vertebrate vCaM

References:

PBO:0013026 - functionally complements S. cerevisiae CMD1

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PBO:0013027 - is not functionally complemented by S. cerevisiae CMD1

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Disease association

MONDO:0013966 - catecholaminergic polymorphic ventricular tachycardia 4

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MONDO:0014548 - long QT syndrome 14

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MONDO:0014550 - long QT syndrome 15

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MONDO:0032915 - long QT syndrome 16

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GO biological process

GO:0030437 - ascospore formation

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GO:0031322 - ascospore-type prospore-specific spindle pole body remodeling

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GO:0090307 - mitotic spindle assembly

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GO:0051300 - spindle pole body organization

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GO cellular component

GO:0051285 - cell cortex of cell tip

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GO:0032153 - cell division site

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GO:0061493 - central plaque of mitotic spindle pole body

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GO:0005829 - cytosol

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GO:0000935 - division septum

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GO:0035838 - growing cell tip

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GO:0043332 - mating projection tip

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GO:0035974 - meiotic spindle pole body

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GO:0044732 - mitotic spindle pole body

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GO:0045160 - myosin I complex

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GO:0035841 - new growing cell tip

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GO:0035840 - old growing cell tip

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GO molecular function

GO:0005509 - calcium ion binding

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GO:0030234 - enzyme regulator activity

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GO:0005515 - protein binding

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Modification

MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

Protein features

PBO:0111825 - calmodulin family

PBO:0111761 - EF hand

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000030 - abnormal mitotic chromosome congression

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Genotypes:

FYPO:0000338 - abnormal mitotic spindle

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Genotypes:

FYPO:0004608 - abnormal spindle pole body morphology during meiosis II

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Genotypes:

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0001915 - abolished prospore membrane formation

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Genotypes:

FYPO:0003542 - abolished protein localization to meiotic spindle pole body

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Genotypes:

FYPO:0000941 - abolished protein localization to mitotic spindle pole body

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Genotypes:

FYPO:0000583 - abolished sporulation

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0004991 - decreased protein level during meiotic cell cycle

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

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Genotypes:

FYPO:0000584 - decreased sporulation frequency

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Genotypes:

FYPO:0002277 - increased protein degradation during nitrogen starvation

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Genotypes:

FYPO:0000316 - inviable after spore germination

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0001489 - inviable vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000283 - mitotic chromosome fragmentation upon segregation

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Genotypes:

FYPO:0000324 - mitotic metaphase/anaphase transition delay

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Genotypes:

FYPO:0004440 - normal cytosolic calcium level

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Genotypes:

FYPO:0002643 - normal growth on clotrimazole

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Genotypes:

FYPO:0002940 - normal growth on micafungin

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Genotypes:

FYPO:0002343 - normal growth on terbinafine

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Genotypes:

FYPO:0001400 - normal interphase microtubules

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Genotypes:

FYPO:0000760 - normal mating

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Genotypes:

FYPO:0000478 - normal meiosis

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Genotypes:

FYPO:0004606 - normal protein level during meiotic cell cycle

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Genotypes:

FYPO:0003541 - normal protein localization to meiotic spindle pole body

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Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

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Genotypes:

FYPO:0000590 - normal sporulation

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Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

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Genotypes:

FYPO:0004434 - sensitive to 4-amino-1-methyl-1H-imidazole-5-carboselenoamide

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Genotypes:

FYPO:0005252 - sensitive to tamoxifen

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Genotypes:

FYPO:0004435 - sensitive to tingenone

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Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

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Genotypes:

FYPO:0001491 - viable vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF13499EF-hand_7EF_hand_domPfam
cd00051EFhEF_hand_domCDD
PS00018EF_HAND_1EF_Hand_1_Ca_BSPROSITE patterns
PS50222EF_HAND_2EF_hand_domPROSITE profiles
SM00054EFhEF_hand_domSMART
G3DSA:1.10.238.10:FF:000527CATH-FunFam
G3DSA:1.10.238.10CATH-Gene3D
SSF47473EF-hand-dom_pairSUPERFAMILY
PTHR23048CALM/Myosin/TropC-likePANTHER

Orthologs

References / Literature

PMID:11260263 - Identification and functional analysis of the gene for type I myosin in fission yeast.
Toya M et al. Genes Cells 2001 Mar;6(3):187-99
PMID:2690071 - Substitution at position 116 of Schizosaccharomyces pombe calmodulin decreases its stability under nitrogen starvation and results in a sporulation-deficient phenotype.
Takeda T et al. Proc Natl Acad Sci U S A 1989 Dec;86(24):9737-41
PMID:15504913 - UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II.
Lord M et al. J Cell Biol 2004 Oct 25;167(2):315-25
PMID:9917066 - Molecular genetic analysis of U2AF59 in Schizosaccharomyces pombe: differential sensitivity of introns to mutational inactivation.
Romfo CM et al. RNA 1999 Jan;5(1):49-65
PMID:15004232 - Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function.
Venkatram S et al. Mol Biol Cell 2004 May;15(5):2287-301
PMID:9635188 - Rng2p, a protein required for cytokinesis in fission yeast, is a component of the actomyosin ring and the spindle pole body.
Eng K et al. Curr Biol 1998 May 21;8(11):611-21
PMID:10617667 - Cloning of a calmodulin kinase I homologue from Schizosaccharomyces pombe.
Rasmussen CD J Biol Chem 2000 Jan 07;275(1):685-90
PMID:7498766 - The role of cdc2 and other genes in meiosis in Schizosaccharomyces pombe.
Iino Y et al. Genetics 1995 Aug;140(4):1235-45
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:37783794 - An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity.
Ader NR et al. Nat Cell Biol 2023 Oct;25(10):1465-1477
PMID:21811607 - Transient receptor potential (TRP) and Cch1-Yam8 channels play key roles in the regulation of cytoplasmic Ca2+ in fission yeast.
Ma Y et al. PLoS One 2011;6(7):e22421
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:28934464 - Recruitment and delivery of the fission yeast Rst2 transcription factor via a local genome structure counteracts repression by Tup1-family corepressors.
Asada R et al. Nucleic Acids Res 2017 Sep 19;45(16):9361-9371
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:18003699 - In vivo movement of the type V myosin Myo52 requires dimerisation but is independent of the neck domain.
Grallert A et al. J Cell Sci 2007 Dec 01;120(Pt 23):4093-8
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:9264467 - Calmodulin localizes to the spindle pole body of Schizosaccharomyces pombe and performs an essential function in chromosome segregation.
Moser MJ et al. J Cell Sci 1997 Aug;110 ( Pt 15):1805-12
PMID:34805795 - The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.
Calvo IA et al. iScience 2021 Nov 19;24(11):103338
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:18204818 - Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants.
Chung KS et al. Invest New Drugs 2008 Aug;26(4):299-307
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:7657644 - Ca2+ binding to calmodulin and its role in Schizosaccharomyces pombe as revealed by mutagenesis and NMR spectroscopy.
Moser MJ et al. J Biol Chem 1995 Sep 01;270(35):20643-52
PMID:38252660 - Metabolic stress-induced long ncRNA transcription governs the formation of meiotic DNA breaks in the fission yeast fbp1 gene.
Tsuruta Y et al. PLoS One 2024;19(1):e0294191
PMID:11864908 - Pcp1p, an Spc110p-related calmodulin target at the centrosome of the fission yeast Schizosaccharomyces pombe.
Flory MR et al. Cell Growth Differ 2002 Feb;13(2):47-58
PMID:20833892 - Schizosaccharomyces pombe calmodulin, Cam1, plays a crucial role in sporulation by recruiting and stabilizing the spindle pole body components responsible for assembly of the forespore membrane.
Itadani A et al. Eukaryot Cell 2010 Dec;9(12):1925-35
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:27432898 - A single-headed fission yeast myosin V transports actin in a tropomyosin-dependent manner.
Tang Q et al. J Cell Biol 2016 Jul 18;214(2):167-79
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26628015 - Characterization of Tamoxifen as an Antifungal Agent Using the Yeast Schizosaccharomyces Pombe Model Organism.
Zhang X et al. Kobe J Med Sci 2015 Oct 09;61(2):E54-63
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:37531259 - Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins.
Willet AH et al. Mol Biol Cell 2023 Oct 01;34(11):br17
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:28619713 - Molecular model of fission yeast centrosome assembly determined by superresolution imaging.
Bestul AJ et al. J Cell Biol 2017 Aug 07;216(8):2409-2424
PMID:38985524 - The core spindle pole body scaffold Ppc89 links the pericentrin ortholog Pcp1 to the fission yeast spindle pole body via an evolutionarily conserved interface.
Chen JS et al. Mol Biol Cell 2024 Jul 10;:mbcE24050220
PMID:3035538 - Analysis and in vivo disruption of the gene coding for calmodulin in Schizosaccharomyces pombe.
Takeda T et al. Proc Natl Acad Sci U S A 1987 Jun;84(11):3580-4
PMID:36095128 - Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2.
Anil AT et al. Nucleic Acids Res 2022 Sep 23;50(17):10000-10014