PomBase home

protein coding gene - cnx1 (SPAC3C7.11c) - calnexin Cnx1

Gene summary

Standard name
cnx1
Systematic ID
SPAC3C7.11c
Product
calnexin Cnx1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
cal1
UniProt ID
P36581
ORFeome ID
35/35B11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2086393..2088324 reverse strand

Annotation

Comment

PBO:0001330 - cells can become independent of the essential calnexin

References:

PBO:0001331 - essential function does not appear to reside in its role in the folding or retention of misfolded proteins

References:

Complementation

PBO:0036284 - functionally complemented by Aspergillus niger clxA

References:

GO biological process

GO:0036503 - ERAD pathway

References:

GO:0006457 - protein folding

References:

GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0005789 - endoplasmic reticulum membrane

References:

GO:0000324 - fungal-type vacuole

References:

GO:0016020 - membrane

References:

GO molecular function

GO:0005509 - calcium ion binding

References:

Miscellaneous functional group

PBO:0000255 - chaperone

Modification

MOD:00689 - disulfide crosslinked residues

References:

MOD:00693 - glycosylated residue

References:

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein sequence feature

SO:0100011 - cleaved_peptide_region

References:

SO:0000418 - signal_peptide

References:

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

PomGeneEx:0000013 - RNA level unchanged

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0006382 - abnormal cell wall organization during nitrogen starvation

References:

Genotypes:

FYPO:0006384 - decreased rate of endocytosis during nitrogen starvation

References:

Genotypes:

FYPO:0006381 - increased cellular reactive oxygen species level during nitrogen starvation

References:

Genotypes:

FYPO:0004164 - increased cellular reactive oxygen species level in stationary phase

References:

Genotypes:

FYPO:0000539 - increased protein secretion during vegetative growth

References:

Genotypes:

FYPO:0001456 - increased RNA level during cellular response to endoplasmic reticulum stress

References:

Genotypes:

FYPO:0002430 - inviable after spore germination, multiple cell divisions

References:

Genotypes:

FYPO:0002107 - inviable stubby vegetative cell

References:

Genotypes:

FYPO:0001489 - inviable vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0001178 - loss of viability upon nitrogen starvation

References:

Genotypes:

FYPO:0000426 - normal endocytosis

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0006380 - normal protein localization to vacuole during nitrogen starvation

References:

Genotypes:

FYPO:0000843 - sensitive to dithiothreitol

References:

Genotypes:

FYPO:0005282 - sensitive to inositol starvation

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00262CalreticulinCalret/calnexPFAM
PS00804CALRETICULIN_2Calret/calnex_CSPROSITE_PATTERNS
PS00803CALRETICULIN_1Calret/calnex_CSPROSITE_PATTERNS
PS00805CALRETICULIN_REPEATCalret/calnex_CSPROSITE_PATTERNS
PR00626CALRETICULINCalret/calnexPRINTS
G3DSA:2.60.120.200:FF:000011FUNFAM
G3DSA:2.10.250.10:FF:000001FUNFAM
SSF49899Concanavalin A-like lectins/glucanasesConA-like_dom_sfSUPERFAMILY
SSF63887P-domain of calnexin/calreticulinCalreticulin/calnexin_P_dom_sfSUPERFAMILY
G3DSA:2.60.120.200GENE3D
G3DSA:2.10.250.10Calreticulin/calnexin, P domainCalreticulin/calnexin_P_dom_sfGENE3D
PTHR11073CALRETICULIN AND CALNEXINCalret/calnexPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:25803873 - Calnexin is essential for survival under nitrogen starvation and stationary phase in Schizosaccharomyces pombe.
Núñez A et al. PLoS One 2015;10(3):e0121059
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:14984369 - Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin.
Maréchal A et al. Biochem J 2004 Jun 01;380(Pt 2):441-8
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.
Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:19606215 - Calnexin regulates apoptosis induced by inositol starvation in fission yeast.
Guérin R et al. PLoS One 2009 Jul 16;4(7):e6244
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:7621821 - The calnexin homologue cnx1+ in Schizosaccharomyces pombe, is an essential gene which can be complemented by its soluble ER domain.
Parlati F et al. EMBO J 1995 Jul 03;14(13):3064-72
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:9860839 - Calnexin and BiP interact with acid phosphatase independently of glucose trimming and reglucosylation in Schizosaccharomyces pombe.
Jannatipour M et al. Biochemistry 1998 Dec 08;37(49):17253-61
PMID:14963023 - A non-chromosomal factor allows viability of Schizosaccharomyces pombe lacking the essential chaperone calnexin.
Collin P et al. J Cell Sci 2004 Feb 29;117(Pt 6):907-18
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:17230581 - The 160 N-terminal residues of calnexin define a novel region supporting viability in Schizosaccharomyces pombe.
Hajjar F et al. Yeast 2007 Feb;24(2):89-103
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:22682253 - Functional repurposing revealed by comparing S. pombe and S. cerevisiae genetic interactions.
Frost A et al. Cell 2012 Jun 08;149(6):1339-52
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:7876257 - The Schizosaccharomyces pombe homologue of the chaperone calnexin is essential for viability.
Jannatipour M et al. J Biol Chem 1995 Mar 03;270(9):4845-53
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:12589443 - Isolation and characterisation of a calnexin homologue, clxA, from Aspergillus niger.
Wang H et al. Mol Genet Genomics 2003 Feb;268(5):684-91
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895