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protein coding gene - gsa1 (SPAC3F10.04) - glutathione synthetase large and small subunit Gsa1

Gene summary

Standard name
gsa1
Systematic ID
SPAC3F10.04
Product
glutathione synthetase large and small subunit Gsa1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
gsh2
UniProt ID
P35669
ORFeome ID
24/24C01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2823730..2827612 forward strand

Annotation

Complementation

PBO:0007272 - functionally complemented by A. thaliana gsh2

References:

Disease association

MONDO:0009947 - glutathione synthetase deficiency with 5-oxoprolinuria

References:

MONDO:0009284 - glutathione synthetase deficiency without 5-oxoprolinuria

References:

MONDO:0017909 - inherited glutathione synthetase deficiency

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GO biological process

GO:0098849 - cellular detoxification of cadmium ion

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GO:0071276 - cellular response to cadmium ion

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GO:0006750 - glutathione biosynthetic process

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GO:0046938 - phytochelatin biosynthetic process

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GO cellular component

GO:0005829 - cytosol

References:

GO:0036087 - glutathione synthase complex

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GO molecular function

GO:0005524 - ATP binding

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GO:0043295 - glutathione binding

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GO:0004363 - glutathione synthase activity

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GO:0000287 - magnesium ion binding

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GO:0005515 - protein binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001570 - decreased cellular glutathione level during vegetative growth

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Genotypes:

FYPO:0003270 - growth auxotrophic for glutathione

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Genotypes:

FYPO:0000741 - increased cellular pigment accumulation

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Protein sequence feature

SO:0100011 - cleaved_peptide_region

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0005883 - abolished anaerobic cell population growth

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Genotypes:

FYPO:0002857 - abolished glutathione synthase activity

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0000249 - decreased cell population growth on ammonia nitrogen source

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Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0009100 - decreased cell population growth on glycerol and galactose carbon source

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0001570 - decreased cellular glutathione level during vegetative growth

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Genotypes:

FYPO:0004007 - decreased protein level during cellular response to menadione

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Genotypes:

FYPO:0003270 - growth auxotrophic for glutathione

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Genotypes:

FYPO:0005882 - increased cellular gamma-glutamyl-2-aminobutyrate level

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Genotypes:

FYPO:0001521 - increased cellular glutathione level during vegetative growth

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Genotypes:

FYPO:0004000 - increased glutamate-cysteine ligase activity

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Genotypes:

FYPO:0003999 - increased glutathione synthase activity

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001753 - normal anaerobic cell population growth

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Genotypes:

FYPO:0004001 - normal cellular glutathione level

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Genotypes:

FYPO:0000973 - normal cellular response to copper ion

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Genotypes:

FYPO:0000974 - normal cellular response to zinc ion

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Genotypes:

FYPO:0001437 - normal growth on antimycin A

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Genotypes:

FYPO:0003506 - normal growth on copper

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000955 - normal growth on lead ion

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Genotypes:

FYPO:0005884 - normal growth on myxothiazol

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Genotypes:

FYPO:0000954 - normal growth on nickel cation

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Genotypes:

FYPO:0001691 - normal growth on paraquat

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Genotypes:

FYPO:0005881 - ophthalmic acid absent from cell

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Genotypes:

FYPO:0002722 - phytochelatin absent from cell

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0009036 - resistance to benzamidine

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0003824 - resistance to caffeine and rapamycin

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Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

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Genotypes:

FYPO:0002634 - resistance to cobalt

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001109 - resistance to menadione

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Genotypes:

FYPO:0001516 - resistance to mercury

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0000093 - sensitive to arsenic

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0000108 - sensitive to menadione

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Subunit composition

PBO:0015230 - heteromeric(3)

References:

PBO:0015212 - homomeric(2)

References:

PBO:0015242 - homomeric(4)

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03917GSH_synth_ATPGlutathione_synthasePFAM
PF03199GSH_synthaseGSH_synth_subst-bdPFAM
cd00228eu-GSGlutathione_synthaseCDD
G3DSA:3.30.1490.50:FF:000002FUNFAM
SSF56059Glutathione synthetase ATP-binding domain-likeSUPERFAMILY
SSF52440PreATP-grasp domainPreATP-grasp_dom_sfSUPERFAMILY
G3DSA:1.10.1080.10Glutathione Synthetase; Chain A, domain 3Glutathione_synthase_a-hlxGENE3D
G3DSA:3.30.1490.80Glutathione_synthase_N_eukGENE3D
G3DSA:3.30.470.20GENE3D
G3DSA:3.40.50.1760GSH-S_sub-bd_sfGENE3D
G3DSA:3.30.1490.50Glutathione_synthase_C_eukGENE3D
PTHR11130GLUTATHIONE SYNTHETASEGlutathione_synthasePANTHER
PIRSF001558GSHaseGlutathione_synthasePIRSF
TIGR01986glut_syn_eukGlutathione_synthaseNCBIFAM

Orthologs

References / Literature

PMID:27005325 - Diverse fission yeast genes required for responding to oxidative and metal stress: Comparative analysis of glutathione-related and other defense gene deletions.
Pluskal T et al. Genes Cells 2016 Jun;21(6):530-42
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:19180638 - SpOPT1, a member of the oligopeptide family (OPT) of the fission yeast Schizosaccharomyces pombe, is involved in the transport of glutathione through the outer membrane of the cell.
Dworeck T et al. Yeast 2009 Jan;26(1):67-73
PMID:8914526 - Cloning of the cDNA and genomic clones for glutathione synthetase from Arabidopsis thaliana and complementation of a gsh2 mutant in fission yeast.
Wang CL et al. Plant Mol Biol 1996 Sep;31(6):1093-104
PMID:8660701 - A single mouse glutathione synthetase gene encodes six mRNAs with different 5' ends.
Shi ZZ et al. Arch Biochem Biophys 1996 Jul 15;331(2):215-24
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:22806344 - Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast.
Ryuko S et al. Mol Genet Genomics 2012 Aug;287(8):651-62
PMID:12734194 - The glutathione synthetase of Schizosaccharomyces pombe is synthesized as a homodimer but retains full activity when present as a heterotetramer.
Phlippen N et al. J Biol Chem 2003 Oct 10;278(41):40152-61
PMID:12787490 - Regulation of the gene encoding glutathione synthetase from the fission yeast.
Kim SJ et al. J Biochem Mol Biol 2003 May 31;36(3):326-31
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PB_REF:0000003 - Disease Association Curation
PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.
Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:9017391 - apd1+, a gene required for red pigment formation in ade6 mutants of Schizosaccharomyces pombe, encodes an enzyme required for glutathione biosynthesis: a role for glutathione and a glutathione-conjugate pump.
Chaudhuri B et al. Genetics 1997 Jan;145(1):75-83
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:1958212 - Cloning and sequencing of the gene encoding the large subunit of glutathione synthetase of Schizosaccharomyces pombe.
Mutoh N et al. Biochem Biophys Res Commun 1991 Nov 27;181(1):430-6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:8192897 - Glutathione synthetase from the fission yeast. Purification and its unique heteromeric subunit structure.
Nakagawa CW et al. Biochem Cell Biol 1993;71(9-10):447-53
PMID:9291132 - Glutathione synthetase: similarities of the proteins from Schizosaccharomyces pombe and Arabidopsis thaliana.
Wang CL et al. Biochem J 1997 Sep 01;326 ( Pt 2)(Pt 2):563-6
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:15529002 - Transcriptional regulation of glutathione synthetase in the fission yeast Schizosaccharomyces pombe.
Kim SJ et al. Mol Cells 2004 Oct 31;18(2):242-8
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:10219997 - Biosynthesis of phytochelatins in the fission yeast. Phytochelatin synthesis: a second role for the glutathione synthetase gene of Schizosaccharomyces pombe.
Al-Lahham A et al. Yeast 1999 Mar 30;15(5):385-96
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:2894829 - Isolation of mutants of Schizosaccharomyces pombe unable to synthesize cadystin, small cadmium-binding peptides.
Mutoh N et al. Biochem Biophys Res Commun 1988 Feb 29;151(1):32-9
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623