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protein coding gene - abc2 (SPAC3F10.11c) - vacuolar phytochelatin and glutathione S-conjugate ABC family transmembrane transporter Abc2

Gene summary

Standard name
abc2
Systematic ID
SPAC3F10.11c
Product
vacuolar phytochelatin and glutathione S-conjugate ABC family transmembrane transporter Abc2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10185
ORFeome ID
48/48G05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2837121..2841788 reverse strand

Annotation

Disease association

MONDO:0018870 - arterial calcification of infancy

References:

MONDO:0013768 - arterial calcification, generalized, of infancy, 2

References:

MONDO:0009925 - autosomal recessive inherited pseudoxanthoma elasticum

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MONDO:0009380 - Dubin-Johnson syndrome

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MONDO:0030058 - hearing loss, autosomal dominant 77

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MONDO:0008333 - pseudoxanthoma elasticum, forme fruste

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GO biological process

GO:0098849 - cellular detoxification of cadmium ion

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GO:0071996 - glutathione transmembrane import into vacuole

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GO:0036246 - phytochelatin 2 import into vacuole

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GO cellular component

GO:0000324 - fungal-type vacuole

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GO:0000329 - fungal-type vacuole membrane

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GO:0005794 - Golgi apparatus

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GO molecular function

GO:0044604 - ABC-type phytochelatin transporter activity

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GO:0005524 - ATP binding

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GO:0016887 - ATP hydrolysis activity

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Modification

MOD:00006 - N-glycosylated residue

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MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0004911 - abolished glutathione import into vacuole

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Genotypes:

FYPO:0002829 - decreased cellular phytochelatin level

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Genotypes:

FYPO:0002653 - decreased vacuolar import

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Genotypes:

FYPO:0004247 - normal vacuolar morphology during vegetative growth

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Genotypes:

FYPO:0004912 - phytochelatin absent from vacuole

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Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Protein features

PBO:0111874 - ABC transporter family

References:

PBO:0111875 - MDR subfamily

Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000013 - RNA level unchanged

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0002653 - decreased vacuolar import

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Genotypes:

FYPO:0004369 - decreased vacuolar phytochelatin level

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Genotypes:

FYPO:0006012 - elongated cell with abolished shmoo formation

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Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0003849 - normal growth on fluconazole

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Genotypes:

FYPO:0003852 - normal growth on FR901464

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Genotypes:

FYPO:0000979 - normal growth on miconazole

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Genotypes:

FYPO:0003851 - normal growth on MTT

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Genotypes:

FYPO:0003850 - normal growth on oligomycin

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Genotypes:

FYPO:0003719 - normal growth on tributyltin

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009039 - resistance to potassium chloride

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Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000327 - resistance to trichostatin A

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF00005ABC_tranABC_transporter-like_ATP-bdPfam
PF00664ABC_membraneABC1_TM_domPfam
PF24357TMD0_ABCTMD0_ABCPfam
cd03244ABCC_MRP_domain2CDD
cd03250ABCC_MRP_domain1CDD
cd18595ABC_6TM_MRP1_2_3_6_D1_likeCDD
cd18603ABC_6TM_MRP1_2_3_6_D2_likeCDD
PS00211ABC_TRANSPORTER_1ABC_transporter-like_CSPROSITE patterns
PS50893ABC_TRANSPORTER_2ABC_transporter-like_ATP-bdPROSITE profiles
PS50929ABC_TM1FABC1_TM_domPROSITE profiles
SM00382AAAAAA+_ATPaseSMART
G3DSA:1.20.1560.10:FF:000001CATH-FunFam
G3DSA:1.20.1560.10:FF:000078CATH-FunFam
G3DSA:3.40.50.300:FF:000565CATH-FunFam
G3DSA:3.40.50.300:FF:002123CATH-FunFam
G3DSA:1.20.1560.10ABC1_TM_sfCATH-Gene3D
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF52540P-loop_NTPaseSUPERFAMILY
SSF90123ABC1_TM_sfSUPERFAMILY
PTHR24223ABC_transporter_C-likePANTHER
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PB_REF:0000003 - Disease Association Curation
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000002 - Comments
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:21297349 - Bfr1p is responsible for tributyltin resistance in Schizosaccharomyces pombe.
Akiyama K et al. J Toxicol Sci 2011 Jan;36(1):117-20
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22840777 - Analyzing fission yeast multidrug resistance mechanisms to develop a genetically tractable model system for chemical biology.
Kawashima SA et al. Chem Biol 2012 Jul 27;19(7):893-901
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:19915076 - abc3+ encodes an iron-regulated vacuolar ABC-type transporter in Schizosaccharomyces pombe.
Pouliot B et al. Eukaryot Cell 2010 Jan;9(1):59-73
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:16849797 - A survey of all 11 ABC transporters in fission yeast: two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant.
Iwaki T et al. Microbiology (Reading) 2006 Aug;152(Pt 8):2309-2321
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:20937798 - Tonoplast-localized Abc2 transporter mediates phytochelatin accumulation in vacuoles and confers cadmium tolerance.
Mendoza-Cózatl DG et al. J Biol Chem 2010 Dec 24;285(52):40416-26
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72