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protein coding gene - frs2 (SPAC3G9.06) - cytoplasmic phenylalanine-tRNA ligase alpha subunit Frs2

Gene summary

Standard name
frs2
Systematic ID
SPAC3G9.06
Product
cytoplasmic phenylalanine-tRNA ligase alpha subunit Frs2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O42870
ORFeome ID
36/36B07
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome I: 3171544..3173370 reverse strand

Annotation

Disease association

MONDO:0100220 - Rajab interstitial lung disease with brain calcifications 2

References:

GO biological process

GO:0002181 - cytoplasmic translation

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0009328 - phenylalanine-tRNA ligase complex

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0000287 - magnesium ion binding

References:

GO:0004826 - phenylalanine-tRNA ligase activity

References:

GO:0000049 - tRNA binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0001890 - increased RNA level

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01409tRNA-synt_2dPhenylalanyl-tRNA_SynthasePfam
PF18553PheRS_DBD3PheRS_DBD3Pfam
cd00496PheRS_alpha_coreCDD
PS50862AA_TRNA_LIGASE_IIaa-tRNA-synth_IIPROSITE profiles
G3DSA:1.10.10.2330:FF:000002CATH-FunFam
G3DSA:3.30.930.10:FF:000028CATH-FunFam
G3DSA:1.10.10.2320CATH-Gene3D
G3DSA:1.10.10.2330CATH-Gene3D
G3DSA:3.30.1370.240CATH-Gene3D
G3DSA:3.30.930.10aa-tRNA-synth_II/BPL/LPLCATH-Gene3D
SSF55681aa-tRNA-synth_II/BPL/LPLSUPERFAMILY
PTHR11538PANTHER
NF003210pheSNCBIFAM
TIGR00468pheSPhe-tRNA-synth_IIc_asuNCBIFAM

Orthologs

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
GO_REF:0000002 - Comments
PMID:34805795 - The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.
Calvo IA et al. iScience 2021 Nov 19;24(11):103338
PMID:29596413 - Lack of 2'-O-methylation in the tRNA anticodon loop of two phylogenetically distant yeast species activates the general amino acid control pathway.
Han L et al. PLoS Genet 2018 Mar;14(3):e1007288
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000051 - S. pombe keyword mapping
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053