protein coding gene - pol1 (SPAC3H5.06c) - DNA polymerase alpha catalytic subunit

Gene summary

Standard name

pol1

Systematic ID

SPAC3H5.06c

Product

DNA polymerase alpha catalytic subunit

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

swi7, polA

UniProt ID

P28040

ORFeome ID

49/49H03

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 3430955..3436241 forward strand

Annotation

Protein features

Orthologs

• POLA1 (HGNC:9173) Homo sapiens
• pol1 (SJAG_05239) Schizosaccharomyces japonicus
• POL1 (YNL102W) Saccharomyces cerevisiae

References / Literature

PMID:15579205 - Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27.

Gray FC et al. BMC Mol Biol 2004 Dec 03;5(1):21

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:9135148 - p56(chk1) protein kinase is required for the DNA replication checkpoint at 37 degrees C in fission yeast.

Francesconi S et al. EMBO J 1997 Mar 17;16(6):1332-41

PMID:24719968 - Switching genes in Schizosaccharomyces pombe.

Gutz H et al. Curr Genet 1985;9(5):325-31

PMID:11581276 - Chromodomain protein Swi6-mediated role of DNA polymerase alpha in establishment of silencing in fission Yeast.

Ahmed S et al. J Biol Chem 2001 Dec 21;276(51):47814-21

PMID:6587363 - Genes required for initiation and resolution steps of mating-type switching in fission yeast.

Egel R et al. Proc Natl Acad Sci U S A 1984 Jun;81(11):3481-5

PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.

Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24

PMID:9710635 - A novel function of the DNA repair gene rhp6 in mating-type silencing by chromatin remodeling in fission yeast.

Singh J et al. Mol Cell Biol 1998 Sep;18(9):5511-22

PMID:8537450 - DNA polymerase alpha, a component of the replication initiation complex, is essential for the checkpoint coupling S phase to mitosis in fission yeast.

D'Urso G et al. J Cell Sci 1995 Sep;108 ( Pt 9):3109-18

PMID:11160827 - Role of fission yeast primase catalytic subunit in the replication checkpoint.

Griffiths DJ et al. Mol Biol Cell 2001 Jan;12(1):115-28

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:8367300 - Fission yeast with DNA polymerase delta temperature-sensitive alleles exhibits cell division cycle phenotype.

Francesconi S et al. Nucleic Acids Res 1993 Aug 11;21(16):3821-8

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:10388806 - Rereplication phenomenon in fission yeast requires MCM proteins and other S phase genes.

Snaith HA et al. Genetics 1999 Jul;152(3):839-51

PMID:30796050 - Ssu72 phosphatase is a conserved telomere replication terminator.

Escandell JM et al. EMBO J 2019 Apr 01;38(7)

PMID:10888871 - Mitotic replication initiation proteins are not required for pre-meiotic S phase.

Forsburg SL et al. Nat Genet 2000 Jul;25(3):263-8

PMID:1291234 - Expression of the catalytic subunits of pol alpha and pol delta from fission yeast Schizosaccharomyces pombe.

Pignède G et al. Chromosoma 1992;102(1 Suppl):S128-32

PMID:19205745 - Redundant roles of Srs2 helicase and replication checkpoint in survival and rDNA maintenance in Schizosaccharomyces pombe.

Yasuhira S Mol Genet Genomics 2009 May;281(5):497-509

PB_REF:0000003 - Disease Association Curation

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:8590799 - The N-terminus of fission yeast DNA polymerase alpha contains a basic pentapeptide that acts in vivo as a nuclear localization signal.

Bouvier D et al. Mol Biol Cell 1995 Dec;6(12):1697-705

PMID:9407031 - Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1.

Saka Y et al. Genes Dev 1997 Dec 15;11(24):3387-400

PMID:19214192 - Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.

Moser BA et al. EMBO J 2009 Apr 08;28(7):810-20

PMID:19109429 - GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.

Pai CC et al. Mol Biol Cell 2009 Feb;20(4):1213-22

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:10716938 - Fission yeast switches mating type by a replication-recombination coupled process.

Arcangioli B et al. EMBO J 2000 Mar 15;19(6):1389-96

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:8319772 - In vivo phosphorylation, mitotic behavior, and nuclear binding of the catalytic subunit of DNA polymerase alpha in fission yeast.

Bouvier D et al. Exp Cell Res 1993 Jul;207(1):41-7

PMID:11344166 - Essential role of Sna41/Cdc45 in loading of DNA polymerase alpha onto minichromosome maintenance proteins in fission yeast.

Uchiyama M et al. J Biol Chem 2001 Jul 13;276(28):26189-96

PMID:26436826 - Polymerase δ replicates both strands after homologous recombination-dependent fork restart.

Miyabe I et al. Nat Struct Mol Biol 2015 Nov;22(11):932-8

PMID:12697806 - Replication proteins influence the maintenance of telomere length and telomerase protein stability.

Dahlén M et al. Mol Cell Biol 2003 May;23(9):3031-42

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:9693370 - Mutational effect of fission yeast polalpha on cell cycle events.

Bhaumik D et al. Mol Biol Cell 1998 Aug;9(8):2107-23

PMID:39094566 - Mrc1 regulates parental histone segregation and heterochromatin inheritance.

Toda T et al. Mol Cell 2024 Jul 23;

PMID:14766746 - Primer utilization by DNA polymerase alpha-primase is influenced by its interaction with Mcm10p.

Fien K et al. J Biol Chem 2004 Apr 16;279(16):16144-53

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.

Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53

PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.

McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238

PMID:25822347 - Role of swi7H4 mutant allele of DNA polymerase α in the DNA damage checkpoint response.

Khan S et al. PLoS One 2015;10(3):e0124063

PMID:15314153 - The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.

Uchiyama M et al. Mol Cell Biol 2004 Sep;24(17):7419-34

PMID:14739927 - Regulation of checkpoint kinases through dynamic interaction with Crb2.

Mochida S et al. EMBO J 2004 Jan 28;23(2):418-28

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:1729129 - DNA polymerase alpha in the fission yeast Schizosaccharomyces pombe: identification and tracing of the catalytic subunit during the cell cycle.

Bouvier D et al. Exp Cell Res 1992 Feb;198(2):183-90

PMID:26041456 - RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends.

Audry J et al. EMBO J 2015 Jul 14;34(14):1942-58

PMID:8423854 - DNA polymerase-alpha is essential for mating-type switching in fission yeast.

Singh J et al. Nature 1993 Jan 21;361(6409):271-3

PMID:8443413 - Cell cycle expression of two replicative DNA polymerases alpha and delta from Schizosaccharomyces pombe.

Park H et al. Mol Biol Cell 1993 Feb;4(2):145-57

PMID:9154809 - A novel mutant allele of Schizosaccharomyces pombe rad26 defective in monitoring S-phase progression to prevent premature mitosis.

Uchiyama M et al. Mol Cell Biol 1997 Jun;17(6):3103-15

PMID:23071723 - DNA polymerase α (swi7) and the flap endonuclease Fen1 (rad2) act together in the S-phase alkylation damage response in S. pombe.

Koulintchenko M et al. PLoS One 2012;7(10):e47091

PMID:11313455 - Fission yeast Rad17 associates with chromatin in response to aberrant genomic structures.

Kai M et al. Mol Cell Biol 2001 May;21(10):3289-301

PMID:15643072 - Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival.

Williams DR et al. Eukaryot Cell 2005 Jan;4(1):166-77

PMID:11027257 - Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation.

Tan S et al. Mol Cell Biol 2000 Nov;20(21):7853-66

PMID:15915339 - Genetic and physical interactions between Schizosaccharomyces pombe Mcl1 and Rad2, Dna2 and DNA polymerase alpha: evidence for a multifunctional role of Mcl1 in DNA replication and repair.

Tsutsui Y et al. Curr Genet 2005 Jul;48(1):34-43

PMID:18615848 - Role of heterochromatin in suppressing subtelomeric recombination in fission yeast.

Bisht KK et al. Yeast 2008 Aug;25(8):537-48

PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.

Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80

PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.

Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:10559981 - A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins.

Edwards RJ et al. Nat Cell Biol 1999 Nov;1(7):393-8

PMID:9891047 - Mutator phenotype induced by aberrant replication.

Liu VF et al. Mol Cell Biol 1999 Feb;19(2):1126-35

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:8246900 - In vivo species specificity of DNA polymerase alpha.

Francesconi S et al. Mol Gen Genet 1993 Nov;241(3-4):457-66

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:12514100 - Checkpoint activation regulates mutagenic translesion synthesis.

Kai M et al. Genes Dev 2003 Jan 01;17(1):64-76

PMID:12455694 - mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation.

Williams DR et al. Eukaryot Cell 2002 Oct;1(5):758-73

PMID:17209013 - Role of SUMO in the dynamics of telomere maintenance in fission yeast.

Xhemalce B et al. Proc Natl Acad Sci U S A 2007 Jan 16;104(3):893-8

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:15805465 - Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress.

Kai M et al. Genes Dev 2005 Apr 15;19(8):919-32

PMID:29774234 - The fission yeast Stn1-Ten1 complex limits telomerase activity via its SUMO-interacting motif and promotes telomeres replication.

Matmati S et al. Sci Adv 2018 May;4(5):eaar2740

PMID:36138017 - Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress.

Wang X et al. Nat Commun 2022 Sep 22;13(1):5565

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.

Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82

PMID:7501454 - Studies of Schizosaccharomyces pombe DNA polymerase alpha at different stages of the cell cycle.

Park H et al. Nucleic Acids Res 1995 Nov 11;23(21):4337-44

PMID:7723827 - A kinase from fission yeast responsible for blocking mitosis in S phase.

Murakami H et al. Nature 1995 Apr 27;374(6525):817-9

PMID:18493607 - A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.

Natsume T et al. PLoS One 2008 May 21;3(5):e2221

PMID:18505873 - Schizosaccharomyces pombe histone acetyltransferase Mst1 (KAT5) is an essential protein required for damage response and chromosome segregation.

Gómez EB et al. Genetics 2008 Jun;179(2):757-71

PMID:9356477 - Schizosaccharomyces pombe cdc20+ encodes DNA polymerase epsilon and is required for chromosomal replication but not for the S phase checkpoint.

D'Urso G et al. Proc Natl Acad Sci U S A 1997 Nov 11;94(23):12491-6

PMID:11073977 - Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1.

Boddy MN et al. Mol Cell Biol 2000 Dec;20(23):8758-66

PMID:30759238 - Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.

Singh B et al. Nucleic Acids Res 2019 Apr 23;47(7):3422-3433

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:9928931 - The S/M checkpoint at 37 degrees C and the recovery of viability of the mutant poldeltats3 require the crb2+/rhp9+ gene in fission yeast.

Grenon M et al. Mol Gen Genet 1999 Jan;260(6):522-34

PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.

Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626

PMID:11387218 - A role for DNA polymerase alpha in epigenetic control of transcriptional silencing in fission yeast.

Nakayama Ji et al. EMBO J 2001 Jun 01;20(11):2857-66

GO_REF:0000002 - Comments

PMID:32355220 - DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres.

Onaka AT et al. Commun Biol 2020 Apr 30;3(1):202

PMID:10588642 - Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assembly.

Pasion SG et al. Mol Biol Cell 1999 Dec;10(12):4043-57

PMID:33568651 - Replication dynamics of recombination-dependent replication forks.

Naiman K et al. Nat Commun 2021 Feb 10;12(1):923

PMID:25691662 - Casein kinase 1γ ensures monopolar growth polarity under incomplete DNA replication downstream of Cds1 and calcineurin in fission yeast.

Koyano T et al. Mol Cell Biol 2015 May;35(9):1533-42

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514