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protein coding gene - pik3 (SPAC458.05) - phosphatidylinositol 3-kinase Pik3

Gene summary

Standard name
pik3
Systematic ID
SPAC458.05
Product
phosphatidylinositol 3-kinase Pik3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
vps34
UniProt ID
P50520
ORFeome ID
28/28F06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4740117..4742814 forward strand

Annotation

Comment

PBO:0011117 - FYVE domain recruiting

References:

GO biological process

GO:0031321 - ascospore-type prospore assembly

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GO:0032120 - ascospore-type prospore membrane formation

References:

GO:0000045 - autophagosome assembly

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GO:0006897 - endocytosis

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GO:0016236 - macroautophagy

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GO:0000425 - pexophagy

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GO:0036092 - phosphatidylinositol-3-phosphate biosynthetic process

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GO:0048015 - phosphatidylinositol-mediated signaling

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GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

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GO:0005768 - endosome

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GO:0016020 - membrane

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GO:0005777 - peroxisome

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GO:0000407 - phagophore assembly site

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GO:0034271 - phosphatidylinositol 3-kinase complex, class III, type I

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GO:0034272 - phosphatidylinositol 3-kinase complex, class III, type II

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GO molecular function

GO:0016303 - 1-phosphatidylinositol-3-kinase activity

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GO:0005515 - protein binding

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Multi-locus phenotype

FYPO:0006623 - decreased phosphatidylinositol-3,4,5-trisphosphate level in plasma membrane

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Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000175 - abnormal ascospore wall assembly

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Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0002256 - abnormal nuclear morphology

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Genotypes:

FYPO:0000942 - abnormal nucleus positioning in prospore

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Genotypes:

FYPO:0001914 - abnormal prospore membrane formation

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Genotypes:

FYPO:0000677 - abnormal protein localization to prospore membrane

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Genotypes:

FYPO:0000676 - abnormal protein targeting to vacuole

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Genotypes:

FYPO:0001118 - abnormal vegetative cell morphology

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Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

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Genotypes:

FYPO:0006295 - abolished macroautophagy during nitrogen starvation

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Genotypes:

FYPO:0004484 - abolished protein localization to prospore membrane

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Genotypes:

FYPO:0004250 - abolished protein localization to vacuolar membrane

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Genotypes:

FYPO:0001669 - abolished protein processing during vegetative growth

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000993 - decreased cellular phosphatidylinositol-3-phosphate level

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0004954 - excess prospore membrane present

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Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0000994 - increased cellular phosphatidylinositol-4-phosphate level

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Genotypes:

FYPO:0000539 - increased protein secretion during vegetative growth

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Genotypes:

FYPO:0000307 - inviable small spore

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Genotypes:

FYPO:0002150 - inviable spore population

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Genotypes:

FYPO:0002427 - inviable spore with abnormal shape

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Genotypes:

FYPO:0000123 - large vacuoles during vegetative growth

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Genotypes:

FYPO:0002795 - large vacuoles present in decreased numbers during vegetative growth

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001369 - mislocalized actomyosin contractile ring

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Genotypes:

FYPO:0002071 - mislocalized nucleus during vegetative growth

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Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

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Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

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Genotypes:

FYPO:0000478 - normal meiosis

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

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Genotypes:

FYPO:0003840 - sensitive to carbendazim

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0000107 - sensitive to latrunculin A

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0003116 - sensitive to plumbagin

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000271 - sensitive to salt stress

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0001510 - viable vegetative cell, abnormal cell shape, normal cell size

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00454PI3_PI4_kinasePI3/4_kinase_cat_domPfam
PF00613PI3KaPI3K_accessory_domPfam
PF00792PI3K_C2PI3K-type_C2_domPfam
cd00870PI3Ka_IIICDD
cd00896PI3Kc_IIIPI3-kinase_type3/VPS34_catCDD
PS00915PI3_4_KINASE_1PI3/4_kinase_CSPROSITE patterns
PS00916PI3_4_KINASE_2PI3/4_kinase_CSPROSITE patterns
PS50290PI3_4_KINASE_3PI3/4_kinase_cat_domPROSITE profiles
PS51545PIK_HELICALPI3K_accessory_domPROSITE profiles
PS51547C2_PI3KPI3K-type_C2_domPROSITE profiles
SM00142PI3K_C2PI3K-type_C2_domSMART
SM00145PI3KaPI3K_accessory_domSMART
SM00146PI3KcPI3/4_kinase_cat_domSMART
G3DSA:1.10.1070.11:FF:000002CATH-FunFam
G3DSA:1.25.40.70:FF:000009CATH-FunFam
G3DSA:3.30.1010.10:FF:000002CATH-FunFam
G3DSA:1.10.1070.11PI3/4_kinase_cat_sfCATH-Gene3D
G3DSA:1.25.40.70PI3K_accessory_sfCATH-Gene3D
G3DSA:2.60.40.150C2_domain_sfCATH-Gene3D
G3DSA:3.30.1010.10CATH-Gene3D
SSF48371ARM-type_foldSUPERFAMILY
SSF49562C2_domain_sfSUPERFAMILY
SSF56112Kinase-like_dom_sfSUPERFAMILY
PTHR10048PI3/4_kinasePANTHER
PIRSF000587PI3K_Vps34PI3K_Vps34PIRSF

Orthologs

References / Literature

PMID:19669754 - Enhanced protein secretion from multiprotease-deficient fission yeast by modification of its vacuolar protein sorting pathway.
Idiris A et al. Appl Microbiol Biotechnol 2010 Jan;85(3):667-77
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21757403 - Schizosaccharomyces pombe Pep12p is required for vacuolar protein transport and vacuolar homotypic fusion.
Hosomi A et al. J Biosci Bioeng 2011 Oct;112(4):309-14
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:31941401 - Atg38-Atg8 interaction in fission yeast establishes a positive feedback loop to promote autophagy.
Yu ZQ et al. Autophagy 2020 Nov;16(11):2036-2051
PMID:39010328 - Fission yeast Pib2 localizes to vacuolar membranes and regulates TOR complex 1 through evolutionarily conserved domains.
Morozumi Y et al. FEBS Lett 2024 Jul 15;
PMID:8719881 - Schizosaccharomyces pombe Vps34p, a phosphatidylinositol-specific PI 3-kinase essential for normal cell growth and vacuole morphology.
Takegawa K et al. J Cell Sci 1995 Dec;108 ( Pt 12):3745-56
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:27974503 - Traffic Through the Trans-Golgi Network and the Endosomal System Requires Collaboration Between Exomer and Clathrin Adaptors in Fission Yeast.
Hoya M et al. Genetics 2017 Feb;205(2):673-690
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:9209031 - Vacuolar protein sorting in fission yeast: cloning, biosynthesis, transport, and processing of carboxypeptidase Y from Schizosaccharomyces pombe.
Tabuchi M et al. J Bacteriol 1997 Jul;179(13):4179-89
PMID:12557273 - Role of phosphatidylinositol 3-phosphate in formation of forespore membrane in Schizosaccharomyces pombe.
Onishi M et al. Yeast 2003 Feb;20(3):193-206
PMID:15249580 - A novel phosphatidylinositol(3,4,5)P3 pathway in fission yeast.
Mitra P et al. J Cell Biol 2004 Jul 19;166(2):205-11
PMID:29975157 - Analysis of the contribution of phosphoinositides to medial septation in fission yeast highlights the importance of PI(4,5)P 2 for medial contractile ring anchoring.
Snider CE et al. Mol Biol Cell 2018 Sep 01;29(18):2148-2155
PMID:25378562 - Vsl1p cooperates with Fsv1p for vacuolar protein transport and homotypic fusion in Schizosaccharomyces pombe.
Hosomi A et al. Microbiology (Reading) 2015 Jan;161(Pt 1):89-98
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:14575697 - Identification of a SNARE protein required for vacuolar protein transport in Schizosaccharomyces pombe.
Takegawa K et al. Biochem Biophys Res Commun 2003 Nov 07;311(1):77-82
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.
Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69
PMID:7565716 - A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development.
Zhou K et al. Mol Cell Biol 1995 Oct;15(10):5645-56
PMID:15189449 - Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe.
Koga T et al. Genes Cells 2004 Jun;9(6):561-74
PMID:17951524 - Schizosaccharomyces pombe Sst4p, a conserved Vps27/Hrs homolog, functions downstream of phosphatidylinositol 3-kinase Pik3p to mediate proper spore formation.
Onishi M et al. Eukaryot Cell 2007 Dec;6(12):2343-53
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:34499173 - Visual detection of binary, ternary and quaternary protein interactions in fission yeast using a Pil1 co-tethering assay.
Yu ZQ et al. J Cell Sci 2021 Oct 01;134(19)
PMID:7772832 - Phosphatidylinositol-3 kinase in fission yeast: a possible role in stress responses.
Kimura K et al. Biosci Biotechnol Biochem 1995 Apr;59(4):678-82
PMID:23028742 - The natural anticancer agent plumbagin induces potent cytotoxicity in MCF-7 human breast cancer cells by inhibiting a PI-5 kinase for ROS generation.
Lee JH et al. PLoS One 2012;7(9):e45023
PMID:25552606 - Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. G3 (Bethesda) 2014 Dec 31;5(3):361-70