protein coding gene - isp6 (SPAC4A8.04) - vacuolar serine protease Isp6

Gene summary

Standard name

isp6

Systematic ID

SPAC4A8.04

Product

vacuolar serine protease Isp6

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

prb1

UniProt ID

P40903

ORFeome ID

21/21F12

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 2544842..2546981 forward strand

Annotation

Protein features

Orthologs

• PCSK9 (HGNC:20001) Homo sapiens
• isp6 (SJAG_02432) Schizosaccharomyces japonicus
• PRB1 (YEL060C) Saccharomyces cerevisiae
• YSP3 (YOR003W) Saccharomyces cerevisiae

References / Literature

PMID:17881729 - Atf1 is a target of the mitogen-activated protein kinase Pmk1 and regulates cell integrity in fission yeast.

Takada H et al. Mol Biol Cell 2007 Dec;18(12):4794-802

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.

Pan X et al. BMC Genomics 2012 Nov 23;13:662

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:17121544 - Fission yeast Tor2 links nitrogen signals to cell proliferation and acts downstream of the Rheb GTPase.

Uritani M et al. Genes Cells 2006 Dec;11(12):1367-79

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:16802154 - Enhanced productivity of protease-sensitive heterologous proteins by disruption of multiple protease genes in the fission yeast Schizosaccharomyces pombe.

Idiris A et al. Appl Microbiol Biotechnol 2006 Nov;73(2):404-20

PMID:21153812 - Processing and maturation of carboxypeptidase Y and alkaline phosphatase in Schizosaccharomyces pombe.

Mukaiyama H et al. Appl Microbiol Biotechnol 2011 Apr;90(1):203-13

PMID:26365378 - ESCRTs Cooperate with a Selective Autophagy Receptor to Mediate Vacuolar Targeting of Soluble Cargos.

Liu XM et al. Mol Cell 2015 Sep 17;59(6):1035-42

PMID:8312388 - Purification and characterization of the endogenous inhibitor for proteinase B from Schizosaccharomyces pombe.

Escudero B et al. Biochimie 1993;75(10):855-9

PMID:11872168 - Genes for a nuclease and a protease are involved in the drastic decrease in cellular RNA amount in fission yeast cells during nitrogen starvation.

Nakashima A et al. J Biochem 2002 Mar;131(3):391-8

PB_REF:0000003 - Disease Association Curation

PMID:19778961 - Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation.

Mukaiyama H et al. Microbiology (Reading) 2009 Dec;155(Pt 12):3816-3826

PMID:11115118 - Identification of proteases with shared functions to the proprotein processing protease Krp1 in the fission yeast Schizosaccharomyces pombe.

Ladds G et al. Mol Microbiol 2000 Nov;38(4):839-53

PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.

Malecki M et al. Genome Biol 2016 Nov 25;17(1):240

PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.

Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838

PMID:16491466 - Construction of a protease-deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease-sensitive heterologous proteins.

Idiris A et al. Yeast 2006 Jan 30;23(2):83-99

PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.

Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650

PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.

Malecki M et al. Wellcome Open Res 2016;1:12

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:31239353 - Zinc-dependent activation of the Pho8 alkaline phosphatase in Schizosaccharomyces pombe .

Hu YM et al. J Biol Chem 2019 Aug 16;294(33):12392-12404

PMID:33138913 - Atg43 tethers isolation membranes to mitochondria to promote starvation-induced mitophagy in fission yeast.

Fukuda T et al. Elife 2020 Nov 03;9

PMID:34980915 - SignalP 6.0 predicts all five types of signal peptides using protein language models.

Teufel F et al. Nat Biotechnol 2022 Jul;40(7):1023-1025

PMID:39477503 - A DUF3844 domain-containing protein is required for vacuolar protein sorting in Schizosaccharomyces pombe.

Inagawa T et al. J Gen Appl Microbiol 2024 Oct 31;

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:39747188 - PhpC NF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA production.

Srivastav MK et al. Nat Commun 2025 Jan 02;16(1):268

PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.

Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29

PMID:19669754 - Enhanced protein secretion from multiprotease-deficient fission yeast by modification of its vacuolar protein sorting pathway.

Idiris A et al. Appl Microbiol Biotechnol 2010 Jan;85(3):667-77

PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.

Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8

PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.

Sun LL et al. PLoS Genet 2013;9(8):e1003715

PMID:25803873 - Calnexin is essential for survival under nitrogen starvation and stationary phase in Schizosaccharomyces pombe.

Núñez A et al. PLoS One 2015;10(3):e0121059

PMID:17261596 - Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast.

Matsuo T et al. Mol Cell Biol 2007 Apr;27(8):3154-64

PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.

Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.

Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684

PMID:31341193 - Ent3 and GGA adaptors facilitate diverse anterograde and retrograde trafficking events to and from the prevacuolar endosome.

Yanguas F et al. Sci Rep 2019 Jul 24;9(1):10747

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:35320724 - Cdc48 influence on separase levels is independent of mitosis and suggests translational sensitivity of separase.

Vijayakumari D et al. Cell Rep 2022 Mar 22;38(12):110554

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.

Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8

PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.

Paulo JA et al. J Proteomics 2020 Jan 06;210:103531

PMID:16550352 - A starvation-specific serine protease gene, isp6+, is involved in both autophagy and sexual development in Schizosaccharomyces pombe.

Nakashima A et al. Curr Genet 2006 Jun;49(6):403-13

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:33534698 - Tripartite suppression of fission yeast TORC1 signaling by the GATOR1-Sea3 complex, the TSC complex, and Gcn2 kinase.

Fukuda T et al. Elife 2021 Feb 03;10

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:7954893 - Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe.

Sato S et al. Curr Genet 1994 Jul;26(1):31-7

PMID:33788833 - Analysis of the SNARE Stx8 recycling reveals that the retromer-sorting motif has undergone evolutionary divergence.

Yanguas F et al. PLoS Genet 2021 Mar;17(3):e1009463

PMID:17295836 - Fission yeast autophagy induced by nitrogen starvation generates a nitrogen source that drives adaptation processes.

Kohda TA et al. Genes Cells 2007 Feb;12(2):155-70