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protein coding gene - vrs2 (SPAC4A8.08c) - mitochondrial valine-tRNA ligase Vrs2/Vas2

Gene summary

Standard name
vrs2
Systematic ID
SPAC4A8.08c
Product
mitochondrial valine-tRNA ligase Vrs2/Vas2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
vas2, ValRS2
UniProt ID
O14160
ORFeome ID
47/47H03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2558454..2561812 reverse strand

Annotation

Complementation

PBO:0011986 - does not functionally complement S. cerevisiae VAS1

References:

Disease association

MONDO:0014397 - combined oxidative phosphorylation defect type 20

References:

GO biological process

GO:0032543 - mitochondrial translation

References:

GO:0006438 - valyl-tRNA aminoacylation

References:

GO cellular component

GO:0005759 - mitochondrial matrix

References:

GO:0005739 - mitochondrion

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GO molecular function

GO:0002161 - aminoacyl-tRNA deacylase activity

References:

GO:0005524 - ATP binding

References:

GO:0004832 - valine-tRNA ligase activity

References:

Modification

MOD:00595 - monomannosylated residue

References:

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0004944 - decreased mitochondrial membrane potential

References:

Genotypes:

FYPO:0007610 - decreased mitochondrial volume

References:

Genotypes:

FYPO:0001110 - inviable after spore germination, multiple cell divisions, normal morphology

References:

Genotypes:

FYPO:0002111 - inviable tapered vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002199 - inviable vegetative cell with normal cell morphology

References:

Genotypes:

FYPO:0006660 - loss of viability upon G0 to G1 transition

References:

Genotypes:

FYPO:0003896 - normal mitochondrial morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00133tRNA-synt_1aa-tRNA-synth_IaPFAM
PF08264Anticodon_1M/V/L/I-tRNA-synth_anticd-bdPFAM
cd00817ValRS_coreCDD
cd07962Anticodon_Ia_ValAnticodon_Ia_ValCDD
PS00178AA_TRNA_LIGASE_Iaa-tRNA-synth_I_CSPROSITE_PATTERNS
PR00986TRNASYNTHVALValyl-tRNA_ligasePRINTS
G3DSA:3.90.740.10:FF:000005FUNFAM
SSF47323Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetasestRNAsynth_Ia_anticodon-bdSUPERFAMILY
SSF52374Nucleotidylyl transferaseSUPERFAMILY
SSF50677ValRS/IleRS/LeuRS editing domainVal/Leu/Ile-tRNA-synth_editSUPERFAMILY
G3DSA:1.10.730.10GENE3D
G3DSA:3.90.740.10Val/Leu/Ile-tRNA-synth_editGENE3D
G3DSA:3.40.50.620HUPsRossmann-like_a/b/a_foldGENE3D
G3DSA:2.170.220.10GENE3D
PTHR11946VALYL-TRNA SYNTHETASESValyl-tRNA_ligasePANTHER
CoilCoilCOILS
TIGR00422valSValyl-tRNA_ligaseNCBIFAM
NF004349PRK05729.1NCBIFAM

Orthologs

References / Literature

PMID:33823662 - Multiple nutritional phenotypes of fission yeast mutants defective in genes encoding essential mitochondrial proteins.
Uehara L et al. Open Biol 2021 Apr;11(4):200369
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:26644575 - Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast.
Halim A et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15648-53
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20106903 - Schizosaccharomyces pombe possesses two paralogous valyl-tRNA synthetase genes of mitochondrial origin.
Chiu WC et al. Mol Biol Evol 2010 Jun;27(6):1415-24
PMID:20976105 - Silencing mediated by the Schizosaccharomyces pombe HIRA complex is dependent upon the Hpc2-like protein, Hip4.
Anderson HE et al. PLoS One 2010 Oct 18;5(10):e13488
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000002 - Comments
PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .
Budden AM et al. Elife 2023 Aug 24;12
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105