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protein coding gene - fas2 (SPAC4A8.11c) - fatty acid synthase alpha subunit Fas2

Gene summary

Standard name
fas2
Systematic ID
SPAC4A8.11c
Product
fatty acid synthase alpha subunit Fas2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
lsd1
UniProt ID
Q10289
ORFeome ID
49/49H08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2566866..2572618 reverse strand

Annotation

GO biological process

GO:0006633 - fatty acid biosynthetic process

References:

GO:0042759 - long-chain fatty acid biosynthetic process

References:

GO:0101026 - mitotic nuclear membrane biogenesis

References:

GO:1900535 - palmitic acid biosynthetic process

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005835 - fatty acid synthase complex

References:

GO molecular function

GO:0004316 - 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity

References:

GO:0004315 - 3-oxoacyl-[acyl-carrier-protein] synthase activity

References:

GO:0000036 - acyl carrier activity

References:

GO:0004312 - fatty acid synthase activity

References:

GO:0004321 - fatty-acyl-CoA synthase activity

References:

GO:0008897 - holo-[acyl-carrier-protein] synthase activity

References:

GO:0000287 - magnesium ion binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00159 - O-phosphopantetheine-L-serine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000720 - abnormal fatty acid synthase activity

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0003286 - decreased mitotic chromosome condensation

References:

Genotypes:

FYPO:0001406 - increased septum thickness

References:

Genotypes:

FYPO:0002303 - inviable mononucleate monoseptate vegetative cell with anucleate compartment

References:

Genotypes:

FYPO:0002251 - inviable swollen elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

References:

Genotypes:

FYPO:0006715 - large and small daughter nuclei

References:

Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0006716 - large and small daughter nuclei, with unequal nuclear envelope distribution

References:

Genotypes:

FYPO:0001390 - misoriented septum during vegetative growth

References:

Genotypes:

FYPO:0000719 - normal fatty acid synthase activity

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Subunit composition

PBO:0034730 - heteromeric(12)

References:

Taxonomic conservation

PBO:0011064 - conserved in fungi

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0001338 - gene name conflict

Protein features

IDNameInterPro nameDB name
PF00106adh_shortSDR_famPfam
PF00109ketoacyl-syntKAS_NPfam
PF01648ACPS4-PPantetheinyl_Trfase_domPfam
PF02801Ketoacyl-synt_CKAS_CPfam
PF18314FAS_I_HFASI_helicalPfam
PF18325Fas_alpha_ACPFas_alpha_ACPPfam
cd00828elong_cond_enzymesFAS_alpha_su_CCDD
cd08950KR_fFAS_SDR_c_likeCDD
PS00606KS3_1Ketoacyl_synth_ASPROSITE patterns
PS50075CARRIERPP-bd_ACPPROSITE profiles
PS52004KS3_2PKS_Beta-ketoAc_synthase_domPROSITE profiles
G3DSA:3.30.70.2490:FF:000001CATH-FunFam
G3DSA:3.40.50.720:FF:000168CATH-FunFam
G3DSA:3.90.25.70:FF:000001CATH-FunFam
G3DSA:3.90.470.20:FF:000005CATH-FunFam
G3DSA:3.30.70.2490CATH-Gene3D
G3DSA:3.40.47.10Thiolase-likeCATH-Gene3D
G3DSA:3.40.50.720CATH-Gene3D
G3DSA:3.90.25.70CATH-Gene3D
G3DSA:3.90.470.204-PPantetheinyl_Trfase_dom_sfCATH-Gene3D
G3DSA:6.10.140.1390CATH-Gene3D
G3DSA:6.10.140.1410CATH-Gene3D
G3DSA:6.10.250.1930CATH-Gene3D
G3DSA:6.10.250.1940CATH-Gene3D
SSF51735NAD(P)-bd_dom_sfSUPERFAMILY
SSF52151Acyl_Trfase/lysoPLipaseSUPERFAMILY
SSF53901Thiolase-likeSUPERFAMILY
SSF562144-PPantetheinyl_Trfase_dom_sfSUPERFAMILY
PTHR10982Fungal_FASPANTHER
PIRSF000454FAS_yeast_alphaFAS_alpha_yeastPIRSF
TIGR00556pantethn_trnPpantetheine-prot_Trfase_domNCBIFAM
MF_00101AcpSACPSHAMAP
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity

Orthologs

References / Literature

GO_REF:0000003 - Gene Ontology annotation based on Enzyme Commission mapping
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.
Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153
PMID:27053105 - Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.
Burr R et al. J Biol Chem 2016 Jun 03;291(23):12171-83
PMID:9693066 - Cloning of the fatty acid synthetase beta subunit from fission yeast, coexpression with the alpha subunit, and purification of the intact multifunctional enzyme complex.
Niwa H et al. Protein Expr Purif 1998 Aug;13(3):403-13
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:11470243 - Very-long-chain fatty acid-containing phospholipids accumulate in fatty acid synthase temperature-sensitive mutant strains of the fission yeast Schizosaccharomyces pombe fas2/lsd1.
Yokoyama K et al. Biochim Biophys Acta 2001 Jun 29;1532(3):223-33
PMID:38482739 - Critical importance of DNA binding for CSL protein functions in fission yeast.
Marešová A et al. J Cell Sci 2024 Mar 14;
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:39378339 - Arrayed CRISPRi library to suppress genes required for Schizosaccharomyces pombe viability.
Ishikawa K et al. J Cell Biol 2025 Jan 06;224(1)
PMID:42691030 - The G-patch protein Cwf28 interacts with the RNA helicase Cdc28 in catalytically active spliceosomes in Schizosaccharomyces pombe.
Karika LO et al. PLoS One 2026;21(9):e0357276
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39652606 - Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast.
Marešová A et al. PLoS Genet 2024 Dec 09;20(12):e1011509
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:38780300 - Nitrogen availability is important for preventing catastrophic mitosis in fission yeast.
Zemlianski V et al. J Cell Sci 2024 May 23;
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:37851576 - The LARP1 homolog Slr1p controls the stability and expression of proto-5'TOP mRNAs in fission yeast.
Mansouri-Noori F et al. Cell Rep 2023 Oct 31;42(10):113226
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:8769419 - Aberrant mitosis in fission yeast mutants defective in fatty acid synthetase and acetyl CoA carboxylase.
Saitoh S et al. J Cell Biol 1996 Aug;134(4):949-61
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .
Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183