PomBase home

protein coding gene - rad31 (SPAC4C5.04) - SUMO activating enzyme E1-type Rad31

Gene summary

Standard name
rad31
Systematic ID
SPAC4C5.04
Product
SUMO activating enzyme E1-type Rad31
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
uba4
UniProt ID
P79064
ORFeome ID
18/18C10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1198391..1199733 forward strand

Annotation

Complementation

PBO:0015661 - functionally complemented by S. cerevisiae AOS1

References:

GO biological process

GO:0007076 - mitotic chromosome condensation

References:

GO:0016925 - protein sumoylation

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0031510 - SUMO activating enzyme complex

References:

GO molecular function

GO:0019948 - SUMO activating enzyme activity

References:

Modification

MOD:01149 - sumoylated lysine

References:

Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111815 - UBA domain protein

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000670 - abnormal mitotic sister chromatid separation

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0002775 - decreased level of sumoylated protein in cell

References:

Genotypes:

FYPO:0002778 - decreased protein sumoylation during vegetative growth

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0000283 - mitotic chromosome fragmentation upon segregation

References:

Genotypes:

FYPO:0002776 - normal level of sumoylated protein in cell

References:

Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00899ThiFThiF_NAD_FAD-bdPfam
cd01492Aos1_SUMOCDD
G3DSA:3.40.50.720CATH-Gene3D
SSF69572Ubiquitin-activating_enzSUPERFAMILY
PTHR10953ThiF/MoeB/HesAPANTHER

Orthologs

References / Literature

PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.
Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:11600706 - SUMO modification of Rad22, the Schizosaccharomyces pombe homologue of the recombination protein Rad52.
Ho JC et al. Nucleic Acids Res 2001 Oct 15;29(20):4179-86
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:33711009 - Structure-function analysis of fission yeast cleavage and polyadenylation factor (CPF) subunit Ppn1 and its interactions with Dis2 and Swd22.
Benjamin B et al. PLoS Genet 2021 Mar;17(3):e1009452
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12
PMID:12597774 - Characterization of SUMO-conjugating enzyme mutants in Schizosaccharomyces pombe identifies a dominant-negative allele that severely reduces SUMO conjugation.
Ho JC et al. Biochem J 2003 May 15;372(Pt 1):97-104
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:28552615 - SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage.
Wei Y et al. Mol Cell 2017 Jun 01;66(5):581-596.e6
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:31072933 - Suppressor screening reveals common kleisin-hinge interaction in condensin and cohesin, but different modes of regulation.
Xu X et al. Proc Natl Acad Sci U S A 2019 May 28;116(22):10889-10898
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:9092625 - Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance.
Shayeghi M et al. Nucleic Acids Res 1997 Mar 15;25(6):1162-9
PMID:15359282 - Role of the fission yeast SUMO E3 ligase Pli1p in centromere and telomere maintenance.
Xhemalce B et al. EMBO J 2004 Oct 01;23(19):3844-53
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83