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protein coding gene - sum1 (SPAC4D7.05) - translation initiation factor eIF3i

Gene summary

Standard name
sum1
Systematic ID
SPAC4D7.05
Product
translation initiation factor eIF3i
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
tif34, tif309
UniProt ID
P79083
ORFeome ID
40/40E05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5358924..5360504 forward strand

Annotation

Complementation

PBO:0015674 - does not functionally complement S. cerevisiae TIF34

References:

PBO:0123310 - is not functionally complemented by human EIF3I

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PBO:0123309 - is not functionally complemented by S. cerevisiae TIF34

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GO biological process

GO:0002183 - cytoplasmic translational initiation

References:

GO:0001732 - formation of cytoplasmic translation initiation complex

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GO cellular component

GO:0005737 - cytoplasm

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GO:0010494 - cytoplasmic stress granule

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GO:0005829 - cytosol

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GO:0016282 - eukaryotic 43S preinitiation complex

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GO:0033290 - eukaryotic 48S preinitiation complex

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GO:0005852 - eukaryotic translation initiation factor 3 complex

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GO:0071540 - eukaryotic translation initiation factor 3 complex, eIF3e

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GO:0071541 - eukaryotic translation initiation factor 3 complex, eIF3m

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GO:0034399 - nuclear periphery

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GO molecular function

GO:0005515 - protein binding

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GO:0003723 - RNA binding

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GO:0003743 - translation initiation factor activity

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Modification

MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001490 - inviable elongated vegetative cell

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Genotypes:

Protein features

PBO:0111751 - WD repeat protein

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000027 - ribosomal density decreased

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PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003402 - abnormal mitotic cell cycle regulation during cellular response to osmotic stress

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0007317 - decreased cytoplasmic translation

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Genotypes:

FYPO:0003125 - decreased cytoplasmic translational initiation

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Genotypes:

FYPO:0001122 - elongated vegetative cell

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Genotypes:

FYPO:0002904 - inviable pear-shaped vegetative cell

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001486 - normal RNA level during cellular response to salt stress

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Genotypes:

FYPO:0003116 - sensitive to plumbagin

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF24805EIF3IeIF3iPFAM
PS00678WD_REPEATS_1WD40_repeat_CSPROSITE_PATTERNS
PS50082WD_REPEATS_2WD40_rptPROSITE_PROFILES
PS50294WD_REPEATS_REGIONPROSITE_PROFILES
SM00320WD40_4WD40_rptSMART
G3DSA:2.130.10.10:FF:000127FUNFAM
SSF50978WD40 repeat-likeWD40_repeat_dom_sfSUPERFAMILY
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfGENE3D
PTHR19877EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT IPANTHER
MF_03008eIF3ieIF3iHAMAP

Orthologs

References / Literature

GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:11705997 - Moe1 and spInt6, the fission yeast homologues of mammalian translation initiation factor 3 subunits p66 (eIF3d) and p48 (eIF3e), respectively, are required for stable association of eIF3 subunits.
Bandyopadhyay A et al. J Biol Chem 2002 Jan 18;277(3):2360-7
PMID:23028742 - The natural anticancer agent plumbagin induces potent cytotoxicity in MCF-7 human breast cancer cells by inhibiting a PI-5 kinase for ROS generation.
Lee JH et al. PLoS One 2012;7(9):e45023
GO_REF:0000104 - Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features.
PMID:21436456 - Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA.
Zhang K et al. Science 2011 Mar 25;331(6024):1624-7
PMID:15904532 - PCI proteins eIF3e and eIF3m define distinct translation initiation factor 3 complexes.
Zhou C et al. BMC Biol 2005 May 17;3:14
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:9560390 - Sum1, a highly conserved WD-repeat protein, suppresses S-M checkpoint mutants and inhibits the osmotic stress cell cycle response in fission yeast.
Humphrey T et al. Genetics 1998 Apr;148(4):1731-42
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:19061185 - Fission yeast translation initiation factor 3 subunit eIF3h is not essential for global translation initiation, but deletion of eif3h+ affects spore formation.
Ray A et al. Yeast 2008 Nov;25(11):809-23
PMID:32071154 - Fission Yeast Puf2, a Pumilio and FBF Family RNA-Binding Protein, Links Stress Granules to Processing Bodies.
Hsiao WY et al. Mol Cell Biol 2020 Apr 13;40(9)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:11134033 - Fission yeast homolog of murine Int-6 protein, encoded by mouse mammary tumor virus integration site, is associated with the conserved core subunits of eukaryotic translation initiation factor 3.
Akiyoshi Y et al. J Biol Chem 2001 Mar 30;276(13):10056-62
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:11071922 - A fission yeast homolog of Int-6, the mammalian oncoprotein and eIF3 subunit, induces drug resistance when overexpressed.
Crane R et al. Mol Biol Cell 2000 Nov;11(11):3993-4003
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:12006658 - Sum1, a component of the fission yeast eIF3 translation initiation complex, is rapidly relocalized during environmental stress and interacts with components of the 26S proteasome.
Dunand-Sauthier I et al. Mol Biol Cell 2002 May;13(5):1626-40