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protein coding gene - met8 (SPAC4D7.06c) - siroheme synthase Met8

Gene summary

Standard name
met8
Systematic ID
SPAC4D7.06c
Product
siroheme synthase Met8
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14172
ORFeome ID
13/13G03
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome I: 5360356..5361574 reverse strand

Annotation

GO biological process

GO:0019354 - siroheme biosynthetic process

References:

GO cellular component

GO:0005829 - cytosol

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GO molecular function

GO:0043115 - precorrin-2 dehydrogenase activity

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GO:0051266 - sirohydrochlorin ferrochelatase activity

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Modification

MOD:00048 - O4'-phospho-L-tyrosine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0000102 - sensitive to cisplatin

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Genotypes:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001934 - abolished cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000037 - growth auxotrophic for cysteine

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Genotypes:

FYPO:0000040 - growth auxotrophic for methionine

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Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0009036 - resistance to benzamidine

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Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000077 - resistance to rapamycin

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0000093 - sensitive to arsenic

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0003384 - sensitive to chromium

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Genotypes:

FYPO:0000102 - sensitive to cisplatin

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF13241NAD_binding_7Pfam
PF14823Sirohm_synth_CMet8_CPfam
PF14824Sirohm_synth_MSirohaem_synthase_centralPfam
G3DSA:1.10.3280.10CATH-Gene3D
G3DSA:3.30.160.110CATH-Gene3D
G3DSA:3.40.50.720CATH-Gene3D
SSF51735NAD(P)-bd_dom_sfSUPERFAMILY
SSF75615SUPERFAMILY
PTHR35330Met8-likePANTHER
TIGR01470cysG_NtermSirohaem_synthase_NNCBIFAM

Orthologs

References / Literature

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.
Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:18665268 - Mitochondrial dysfunction increases oxidative stress and decreases chronological life span in fission yeast.
Zuin A et al. PLoS One 2008 Jul 30;3(7):e2842
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000116 - Automatic Gene Ontology annotation based on Rhea mapping.
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.
Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39