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protein coding gene - csi2 (SPAC4D7.07c) - mitotic chromosome segregation protein Csi2

Gene summary

Standard name
csi2
Systematic ID
SPAC4D7.07c
Product
mitotic chromosome segregation protein Csi2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14173
ORFeome ID
45/45H08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5361612..5363899 reverse strand

Annotation

GO biological process

GO:0007052 - mitotic spindle organization

References:

GO cellular component

GO:0044732 - mitotic spindle pole body

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GO:1990578 - perinuclear endoplasmic reticulum membrane

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Modification

MOD:00006 - N-glycosylated residue

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Multi-locus phenotype

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0004649 - abolished protein localization to mitotic spindle pole body during metaphase

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Genotypes:

FYPO:0004650 - decreased mitotic spindle microtubule depolymerization

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Genotypes:

FYPO:0004648 - delayed onset of mitotic spindle assembly

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Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

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Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

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Genotypes:

FYPO:0004307 - long mitotic spindle during metaphase

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000276 - monopolar mitotic spindle

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Genotypes:

FYPO:0001870 - normal centromere clustering at nuclear periphery during vegetative growth

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Genotypes:

FYPO:0004310 - normal duration of mitotic M phase

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0001400 - normal interphase microtubules

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Genotypes:

FYPO:0002966 - normal protein localization to mitotic spindle

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Genotypes:

FYPO:0002967 - normal protein localization to mitotic spindle pole body

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0006931 - sensitive to butylated hydroxytoluene

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Genotypes:

FYPO:0003840 - sensitive to carbendazim

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0016885 - Schizosaccharomyces specific

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
G3DSA:2.60.120.260GENE3D

Orthologs

References / Literature

PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:25253718 - csi2p modulates microtubule dynamics and organizes the bipolar spindle for chromosome segregation.
Costa J et al. Mol Biol Cell 2014 Dec 01;25(24):3900-8
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:29021344 - A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.
Yukawa M et al. Mol Biol Cell 2017 Dec 01;28(25):3647-3659
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.
Liu NN et al. Cell Res 2010 Aug;20(8):963-5
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663