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protein coding gene - tif223 (SPAC4D7.09) - translation initiation factor eIF2B gamma subunit

Gene summary

Standard name
tif223
Systematic ID
SPAC4D7.09
Product
translation initiation factor eIF2B gamma subunit
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P56288
ORFeome ID
35/35H04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5367405..5369378 forward strand

Annotation

Disease association

MONDO:0957871 - leukoencephalopathy with vanishing white matter 3

References:

MONDO:0005071 - nervous system disorder

References:

GO biological process

GO:0002183 - cytoplasmic translational initiation

References:

GO:0006446 - regulation of translational initiation

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005851 - eukaryotic translation initiation factor 2B complex

References:

GO molecular function

GO:0005085 - guanyl-nucleotide exchange factor activity

References:

GO:0005515 - protein binding

References:

GO:0003743 - translation initiation factor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0003510 - normal growth on silver ions

References:

Genotypes:

FYPO:0006929 - sensitive to silver nanoparticles

References:

Genotypes:

Subunit composition

PBO:0032733 - heteromeric(10)

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0091605 - 2 sequence errors 5368262TC->T 5368273 and G->GT would change amino acids PPIYGL to PPYTVL

References:

PBO:0000108 - frameshifted

References:

PBO:0000070 - gene structure updated

References:

PBO:0000129 - sequence error in genomic data

References:

Protein features

IDNameInterPro nameDB name
PF00483NTP_transferaseNTP_transferase_domPfam
PF25084LbH_EIF2BLbH_EIF2B3/5Pfam
cd04198eIF-2B_gamma_NCDD
cd04652LbH_eIF2B_gamma_CCDD
G3DSA:2.160.10.10:FF:000045CATH-FunFam
G3DSA:2.160.10.10CATH-Gene3D
G3DSA:3.90.550.10Nucleotide-diphossugar_transCATH-Gene3D
SSF53448Nucleotide-diphossugar_transSUPERFAMILY
PTHR45989eIF2B_gammaPANTHER

Orthologs

References / Literature

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:29294138 - Editor's Highlight: A Genome-wide Screening of Target Genes Against Silver Nanoparticles in Fission Yeast.
Lee AR et al. Toxicol Sci 2018 Jan 01;161(1):171-185
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:27023709 - Expression, purification, and crystallization of Schizosaccharomyces pombe eIF2B.
Kashiwagi K et al. J Struct Funct Genomics 2016 Mar;17(1):33-8
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:31048492 - Structural basis for eIF2B inhibition in integrated stress response.
Kashiwagi K et al. Science 2019 May 03;364(6439):495-499
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:34805795 - The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.
Calvo IA et al. iScience 2021 Nov 19;24(11):103338
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PB_REF:0000003 - Disease Association Curation
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:26901872 - Crystal structure of eukaryotic translation initiation factor 2B.
Kashiwagi K et al. Nature 2016 Mar 03;531(7592):122-5
PMID:26615217 - Bulk Segregant Analysis Reveals the Genetic Basis of a Natural Trait Variation in Fission Yeast.
Hu W et al. Genome Biol Evol 2015 Nov 27;7(12):3496-510