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protein coding gene - spt20 (SPAC4D7.10c) - SAGA complex subunit Spt20

Gene summary

Standard name
spt20
Systematic ID
SPAC4D7.10c
Product
SAGA complex subunit Spt20
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14174
ORFeome ID
25/25F03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5369400..5371844 reverse strand

Annotation

Comment

PBO:0000206 - deletion mutant expression profiling

References:

GO biological process

GO:0006357 - regulation of transcription by RNA polymerase II

References:

GO:0045815 - transcription initiation-coupled chromatin remodeling

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GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO:0000124 - SAGA complex

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GO molecular function

GO:0003713 - transcription coactivator activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01149 - sumoylated lysine

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Multi-locus phenotype

FYPO:0006060 - abolished protein localization to septin ring

References:

Genotypes:

FYPO:0000134 - branched, elongated, multiseptate cell

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Genotypes:

FYPO:0006062 - ectopic septin ring assembly during vegetative growth

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Genotypes:

FYPO:0000223 - elongated multiseptate vegetative cell

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Genotypes:

FYPO:0001252 - multinucleate multiseptate vegetative cell

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Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0006399 - abnormal septin ring morphology

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Genotypes:

FYPO:0006060 - abolished protein localization to septin ring

References:

Genotypes:

FYPO:0000134 - branched, elongated, multiseptate cell

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0004903 - decreased level of cell separation after cytokinesis gene mRNA during vegetative growth

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

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Genotypes:

FYPO:0008120 - decreased protein level in SAGA complex

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Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

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Genotypes:

FYPO:0000781 - decreased transcription during vegetative growth

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Genotypes:

FYPO:0006062 - ectopic septin ring assembly during vegetative growth

References:

Genotypes:

FYPO:0000223 - elongated multiseptate vegetative cell

References:

Genotypes:

FYPO:0002317 - increased cellular squalene level

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Genotypes:

FYPO:0000252 - increased spontaneous diploidization

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Genotypes:

FYPO:0001252 - multinucleate multiseptate vegetative cell

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Genotypes:

FYPO:0000962 - normal growth on hydrogen peroxide

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Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0000840 - normal RNA level

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Genotypes:

FYPO:0001216 - normal SAGA complex assembly

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Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

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Genotypes:

FYPO:0000093 - sensitive to arsenic

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000099 - sensitive to canavanine

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0007556 - sensitive to diethylstilbestrol

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Genotypes:

FYPO:0007555 - sensitive to econazole

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0003358 - sensitive to miconazole

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Genotypes:

FYPO:0001207 - sensitive to raffinose

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Genotypes:

FYPO:0000111 - sensitive to rapamycin

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0007554 - sensitive to tolnaftate

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

FYPO:0000280 - sterile

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Genotypes:

FYPO:0002459 - viable branched, elongated vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0001137 - ncRNA in intron

Protein features

IDNameInterPro nameDB name
PF12090Spt20_SEPSpt20-like_SEPPFAM
PTHR13526TRANSCRIPTION FACTOR SPT20 HOMOLOGSpt20PANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:30573453 - Anti-silencing factor Epe1 associates with SAGA to regulate transcription within heterochromatin.
Bao K et al. Genes Dev 2019 Jan 01;33(1-2):116-126
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21642955 - Tra1 has specific regulatory roles, rather than global functions, within the SAGA co-activator complex.
Helmlinger D et al. EMBO J 2011 Jun 03;30(14):2843-52
PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .
Budden AM et al. Elife 2023 Aug 24;12
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:31748520 - Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes in yeast.
Elías-Villalobos A et al. Nat Commun 2019 Nov 20;10(1):5237
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:33223513 - Systematic Target Screening Revealed That Tif302 Could Be an Off-Target of the Antifungal Terbinafine in Fission Yeast.
Lee S et al. Biomol Ther (Seoul) 2021 Mar 01;29(2):234-247
PMID:29079657 - TORC1 and TORC2 converge to regulate the SAGA co-activator in response to nutrient availability.
Laboucarié T et al. EMBO Rep 2017 Dec;18(12):2197-2218
PMID:18042546 - Characterization of zfs1 as an mRNA-binding and -destabilizing protein in Schizosaccharomyces pombe.
Cuthbertson BJ et al. J Biol Chem 2008 Feb 01;283(5):2586-94
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:21760946 - Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.
Takeda K et al. PLoS One 2011;6(7):e22021
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25015293 - Septin ring assembly is regulated by Spt20, a structural subunit of the SAGA complex.
Lei B et al. J Cell Sci 2014 Sep 15;127(Pt 18):4024-36
PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.
Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333
PMID:19056896 - The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8.
Helmlinger D et al. Genes Dev 2008 Nov 15;22(22):3184-95
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:35286199 - Mitotic spindle formation in the absence of Polo kinase.
Kim J et al. Proc Natl Acad Sci U S A 2022 Mar 22;119(12):e2114429119
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6