protein coding gene - rng2 (SPAC4F8.13c) - IQGAP domain cytoskeleton scaffold protein Rng2

Gene summary

Standard name

rng2

Systematic ID

SPAC4F8.13c

Product

IQGAP domain cytoskeleton scaffold protein Rng2

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

O14188

ORFeome ID

30/30H04

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 2645428..2650427 forward strand

Annotation

Protein features

Orthologs

• IQGAP1 (HGNC:6110) Homo sapiens
• IQGAP2 (HGNC:6111) Homo sapiens
• rng2 (SJAG_04498) Schizosaccharomyces japonicus
• IQG1 (YPL242C) Saccharomyces cerevisiae

References / Literature

PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.

Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:21376600 - Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/anillin.

Almonacid M et al. Curr Biol 2011 Mar 22;21(6):473-9

PMID:33496728 - Molecular organization of cytokinesis node predicts the constriction rate of the contractile ring.

Bellingham-Johnstun K et al. J Cell Biol 2021 Mar 01;220(3)

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:38041816 - Unraveling the mechanisms and evolution of a two-domain module in IQGAP proteins for controlling eukaryotic cytokinesis.

Wang K et al. Cell Rep 2023 Nov 30;42(12):113510

PMID:16866873 - Actin-capping protein is involved in controlling organization of actin cytoskeleton together with ADF/cofilin, profilin and F-actin crosslinking proteins in fission yeast.

Nakano K et al. Genes Cells 2006 Aug;11(8):893-905

PMID:9649519 - Isolation and characterization of new fission yeast cytokinesis mutants.

Balasubramanian MK et al. Genetics 1998 Jul;149(3):1265-75

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:19805578 - Genome-wide mapping of myosin protein-RNA networks suggests the existence of specialized protein production sites.

Mata J FASEB J 2010 Feb;24(2):479-84

PMID:22918943 - Anillin-related protein Mid1p coordinates the assembly of the cytokinetic contractile ring in fission yeast.

Saha S et al. Mol Biol Cell 2012 Oct;23(20):3982-92

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:12791993 - Equatorial retention of the contractile actin ring by microtubules during cytokinesis.

Pardo M et al. Science 2003 Jun 06;300(5625):1569-74

PMID:15265986 - The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe.

Mishra M et al. J Cell Sci 2004 Aug 01;117(Pt 17):3897-910

PMID:16772338 - The fission yeast Chs2 protein interacts with the type-II myosin Myo3p and is required for the integrity of the actomyosin ring.

Martín-García R et al. J Cell Sci 2006 Jul 01;119(Pt 13):2768-79

PMID:11294907 - Roles of a fimbrin and an alpha-actinin-like protein in fission yeast cell polarization and cytokinesis.

Wu JQ et al. Mol Biol Cell 2001 Apr;12(4):1061-77

PMID:15272162 - Structure, crystal packing and molecular dynamics of the calponin-homology domain of Schizosaccharomyces pombe Rng2.

Wang CH et al. Acta Crystallogr D Biol Crystallogr 2004 Aug;60(Pt 8):1396-403

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:20603077 - Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.

Roberts-Galbraith RH et al. Mol Cell 2010 Jul 09;39(1):86-99

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:19713940 - Fission yeast IQGAP arranges actin filaments into the cytokinetic contractile ring.

Takaine M et al. EMBO J 2009 Oct 21;28(20):3117-31

PMID:11069761 - The S. pombe rlc1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo3p and myo2p.

Le Goff X et al. J Cell Sci 2000 Dec;113 Pt 23:4157-63

PMID:33131769 - Cell cycle-dependent phosphorylation of IQGAP is involved in assembly and stability of the contractile ring in fission yeast.

Morita R et al. Biochem Biophys Res Commun 2021 Jan 01;534:1026-1032

PMID:10769201 - The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation.

Salimova E et al. J Cell Sci 2000 May;113 ( Pt 10):1695-704

PMID:23615450 - Separate roles of IQGAP Rng2p in forming and constricting the Schizosaccharomyces pombe cytokinetic contractile ring.

Tebbs IR et al. Mol Biol Cell 2013 Jun;24(12):1904-17

PMID:30853434 - NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement.

Willet AH et al. Curr Biol 2019 Mar 18;29(6):1055-1063.e2

PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .

Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:10545452 - Drc1p/Cps1p, a 1,3-beta-glucan synthase subunit, is essential for division septum assembly in Schizosaccharomyces pombe.

Liu J et al. Genetics 1999 Nov;153(3):1193-203

PMID:25688133 - The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc12.

Willet AH et al. J Cell Biol 2015 Feb 16;208(4):391-9

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:24790095 - Characterization of the roles of Blt1p in fission yeast cytokinesis.

Goss JW et al. Mol Biol Cell 2014 Jul 01;25(13):1946-57

PMID:16224022 - Counting cytokinesis proteins globally and locally in fission yeast.

Wu JQ et al. Science 2005 Oct 14;310(5746):310-4

PMID:14501122 - Expression, purification, crystallization and preliminary crystallographic analysis of the calponin-homology domain of Rng2.

Wang CH et al. Acta Crystallogr D Biol Crystallogr 2003 Oct;59(Pt 10):1809-12

PMID:19139265 - The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring.

Roberts-Galbraith RH et al. J Cell Biol 2009 Jan 12;184(1):113-27

PMID:9635188 - Rng2p, a protein required for cytokinesis in fission yeast, is a component of the actomyosin ring and the spindle pole body.

Eng K et al. Curr Biol 1998 May 21;8(11):611-21

PMID:28914606 - Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.

McDonald NA et al. Elife 2017 Sep 15;6

PMID:29343550 - Cdk1-dependent phosphoinhibition of a formin-F-BAR interaction opposes cytokinetic contractile ring formation.

Willet AH et al. Mol Biol Cell 2018 Mar 15;29(6):713-721

PMID:26702831 - Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Motility and Cytokinesis.

McDonald NA et al. Dev Cell 2015 Dec 21;35(6):725-36

PMID:15743909 - Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast.

Kovar DR et al. Mol Biol Cell 2005 May;16(5):2313-24

PMID:18272786 - Pxl1p, a paxillin-related protein, stabilizes the actomyosin ring during cytokinesis in fission yeast.

Ge W et al. Mol Biol Cell 2008 Apr;19(4):1680-92

PMID:8834798 - Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ring.

Chang F et al. J Cell Sci 1996 Jan;109 ( Pt 1):131-42

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:16864655 - Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast.

Wu JQ et al. J Cell Biol 2006 Jul 31;174(3):391-402

PMID:26941334 - Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis.

Wei B et al. Mol Biol Cell 2016 Apr 15;27(8):1235-45

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:31217286 - Increasing ergosterol levels delays formin-dependent assembly of F-actin cables and disrupts division plane positioning in fission yeast.

Arbizzani F et al. J Cell Sci 2019 Jul 01;132(13)

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:21422229 - Assembly and architecture of precursor nodes during fission yeast cytokinesis.

Laporte D et al. J Cell Biol 2011 Mar 21;192(6):1005-21

PMID:34958661 - Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division.

Opalko HE et al. J Cell Biol 2022 Feb 07;221(2)

PMID:24954052 - Formins determine the functional properties of actin filaments in yeast.

Johnson M et al. Curr Biol 2014 Jul 07;24(13):1525-30

PMID:18256290 - Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis.

Pinar M et al. Mol Biol Cell 2008 Apr;19(4):1727-38

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:24127216 - The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis.

Coffman VC et al. J Cell Biol 2013 Oct 14;203(1):101-14

PMID:25977474 - A role for F-BAR protein Rga7p during cytokinesis in S. pombe.

Arasada R et al. J Cell Sci 2015 Jul 01;128(13):2259-68

PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.

Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7

PMID:11942609 - Interactions of Cdc4p, a myosin light chain, with IQ-domain containing proteins in Schizosaccharomyces pombe.

D'souza VM et al. Cell Struct Funct 2001 Dec;26(6):555-65

PMID:21376595 - IQGAP-related Rng2p organizes cortical nodes and ensures position of cell division in fission yeast.

Padmanabhan A et al. Curr Biol 2011 Mar 22;21(6):467-72

PMID:35108037 - Fission yeast paxillin contains two Cdc15 binding motifs for robust recruitment to the cytokinetic ring.

Snider CE et al. Mol Biol Cell 2022 Apr 01;33(4):br4

PMID:11694585 - Interactions among a fimbrin, a capping protein, and an actin-depolymerizing factor in organization of the fission yeast actin cytoskeleton.

Nakano K et al. Mol Biol Cell 2001 Nov;12(11):3515-26

PMID:14602073 - Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis.

Wu JQ et al. Dev Cell 2003 Nov;5(5):723-34

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:23966468 - Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis.

Zhu YH et al. Mol Biol Cell 2013 Oct;24(20):3187-204

PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.

Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72

PMID:22427686 - Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis.

Lee IJ et al. J Cell Sci 2012 Jun 15;125(Pt 12):2973-85

PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.

Mak T et al. EMBO J 2021 Aug 16;40(16):e107911