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protein coding gene - puf5 (SPAC4G8.03c) - cytoplasmic pumilio family RNA-binding protein Puf5

Gene summary

Standard name
puf5
Systematic ID
SPAC4G8.03c
Product
cytoplasmic pumilio family RNA-binding protein Puf5
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09829
ORFeome ID
28/28D08
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome I: 757915..761972 reverse strand

Annotation

GO biological process

GO:0000288 - nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay

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GO cellular component

GO:0005737 - cytoplasm

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GO molecular function

GO:0003729 - mRNA binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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Protein features

PBO:0111797 - pumilio family

Qualitative gene expression

PomGeneEx:0000027 - ribosomal density decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0009035 - resistance to formamide

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0000077 - resistance to rapamycin

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0009065 - resistance to X-rays and rapamycin during vegetative growth

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Genotypes:

FYPO:0009062 - resistance to X-rays during vegetative growth

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

Protein features

IDNameInterPro nameDB name
PF00806PUFPumilio_RNA-bd_rptPFAM
cd07920PumilioPumilio_RNA-bdCDD
PS50302PUMPumilio_RNA-bd_rptPROSITE_PROFILES
PS50303PUM_HDPUM-HDPROSITE_PROFILES
SM00025pum_5Pumilio_RNA-bd_rptSMART
G3DSA:1.25.10.10:FF:000237FUNFAM
SSF48371ARM repeatARM-type_foldSUPERFAMILY
G3DSA:1.25.10.10ARM-likeGENE3D
PTHR12537RNA BINDING PROTEIN PUMILIO-RELATEDPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:32071154 - Fission Yeast Puf2, a Pumilio and FBF Family RNA-Binding Protein, Links Stress Granules to Processing Bodies.
Hsiao WY et al. Mol Cell Biol 2020 Apr 13;40(9)
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:34209806 - Identification of Nrl1 Domains Responsible for Interactions with RNA-Processing Factors and Regulation of Nrl1 Function by Phosphorylation.
Mikolaskova B et al. Int J Mol Sci 2021 Jun 29;22(13)
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405